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[Cross-talk between c-Jun/Ets1 involved in EB virus-encoded latent membrane protein 1 regulates expression of matrix metalloproteinase-9 in nasopharyngeal carcinoma].
Zhonghua Zhong Liu Za Zhi. 2005 Apr; 27(4):204-8.ZZ

Abstract

OBJECTIVE

To investigate effect of AP-1 and Ets binding site adjacent to matrix metalloproteinase-9 (MMP-9) promoter on activation of MMP-9 transcription of nasopharyngeal carcinoma cells transfected with EBV-encoded latent membrane protein 1 (LMP1), and to ascertain if cross-talk between c-Jun and Ets1 is involved in LMP1-regulating expression of MMP-9.

METHODS

Site-directed mutagenesis technique was used to establish a series of mutants, including MMP-9-CAT-Ets(-540)mt, MMP-9-CAT-AP-1(-533)mt and MMP-9-CAT-AP-1(-533)/Ets(-540)mt. After the mutants were transfected into LMP1-expressing NPC HNE2 cells regulated by Tet-on system (pTet-on-LMP1 HNE2), CAT activity of these mutants were assayed with induction of LMP1. With blockade of c-Jun or Ets1 antisense oligonucleotides, the activity of MMP-9 induced by LMP1 was assayed with gelatin zymography.

RESULTS

The CAT activity of MMP-9-Ets(-540)mt-CAT, MMP-9-AP-1(-533)mt-CAT, MMP-9-AP-1(-533)/Ets(-540) mt-CAT decreased significantly compared to MMP-9-CAT wt. After blockade with c-Jun or Ets1 antisense oligonucleotides, activity of MMP-9 induced by LMP1 decreased significantly, especially with combined blockade of c-Jun and Ets1.

CONCLUSION

The results suggest that transcription factor AP-1 and Ets play an crucial role in activation of MMP-9 transcription induced by LMP1, and cross-talk between c-Jun/Ets1 is involved in expression of MMP-9 mediated by LMP1.

Authors+Show Affiliations

Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410078, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

15949416

Citation

Zeng, Liang, et al. "[Cross-talk Between c-Jun/Ets1 Involved in EB Virus-encoded Latent Membrane Protein 1 Regulates Expression of Matrix Metalloproteinase-9 in Nasopharyngeal Carcinoma]." Zhonghua Zhong Liu Za Zhi [Chinese Journal of Oncology], vol. 27, no. 4, 2005, pp. 204-8.
Zeng L, Liu YP, Tao YG, et al. [Cross-talk between c-Jun/Ets1 involved in EB virus-encoded latent membrane protein 1 regulates expression of matrix metalloproteinase-9 in nasopharyngeal carcinoma]. Zhonghua Zhong Liu Za Zhi. 2005;27(4):204-8.
Zeng, L., Liu, Y. P., Tao, Y. G., Ai, M. D., Zhao, X. R., & Cao, Y. (2005). [Cross-talk between c-Jun/Ets1 involved in EB virus-encoded latent membrane protein 1 regulates expression of matrix metalloproteinase-9 in nasopharyngeal carcinoma]. Zhonghua Zhong Liu Za Zhi [Chinese Journal of Oncology], 27(4), 204-8.
Zeng L, et al. [Cross-talk Between c-Jun/Ets1 Involved in EB Virus-encoded Latent Membrane Protein 1 Regulates Expression of Matrix Metalloproteinase-9 in Nasopharyngeal Carcinoma]. Zhonghua Zhong Liu Za Zhi. 2005;27(4):204-8. PubMed PMID: 15949416.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Cross-talk between c-Jun/Ets1 involved in EB virus-encoded latent membrane protein 1 regulates expression of matrix metalloproteinase-9 in nasopharyngeal carcinoma]. AU - Zeng,Liang, AU - Liu,Yi-ping, AU - Tao,Yong-guang, AU - Ai,Mi-dan, AU - Zhao,Xiao-rong, AU - Cao,Ya, PY - 2005/6/14/pubmed PY - 2007/9/29/medline PY - 2005/6/14/entrez SP - 204 EP - 8 JF - Zhonghua zhong liu za zhi [Chinese journal of oncology] JO - Zhonghua Zhong Liu Za Zhi VL - 27 IS - 4 N2 - OBJECTIVE: To investigate effect of AP-1 and Ets binding site adjacent to matrix metalloproteinase-9 (MMP-9) promoter on activation of MMP-9 transcription of nasopharyngeal carcinoma cells transfected with EBV-encoded latent membrane protein 1 (LMP1), and to ascertain if cross-talk between c-Jun and Ets1 is involved in LMP1-regulating expression of MMP-9. METHODS: Site-directed mutagenesis technique was used to establish a series of mutants, including MMP-9-CAT-Ets(-540)mt, MMP-9-CAT-AP-1(-533)mt and MMP-9-CAT-AP-1(-533)/Ets(-540)mt. After the mutants were transfected into LMP1-expressing NPC HNE2 cells regulated by Tet-on system (pTet-on-LMP1 HNE2), CAT activity of these mutants were assayed with induction of LMP1. With blockade of c-Jun or Ets1 antisense oligonucleotides, the activity of MMP-9 induced by LMP1 was assayed with gelatin zymography. RESULTS: The CAT activity of MMP-9-Ets(-540)mt-CAT, MMP-9-AP-1(-533)mt-CAT, MMP-9-AP-1(-533)/Ets(-540) mt-CAT decreased significantly compared to MMP-9-CAT wt. After blockade with c-Jun or Ets1 antisense oligonucleotides, activity of MMP-9 induced by LMP1 decreased significantly, especially with combined blockade of c-Jun and Ets1. CONCLUSION: The results suggest that transcription factor AP-1 and Ets play an crucial role in activation of MMP-9 transcription induced by LMP1, and cross-talk between c-Jun/Ets1 is involved in expression of MMP-9 mediated by LMP1. SN - 0253-3766 UR - https://www.unboundmedicine.com/medline/citation/15949416/[Cross_talk_between_c_Jun/Ets1_involved_in_EB_virus_encoded_latent_membrane_protein_1_regulates_expression_of_matrix_metalloproteinase_9_in_nasopharyngeal_carcinoma]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&issn=0253-3766&year=2005&vol=27&issue=4&fpage=204 DB - PRIME DP - Unbound Medicine ER -