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Interdependence between transcription and mRNP processing and export, and its impact on genetic stability.
Mol Cell. 2005 Jun 10; 18(6):711-22.MC

Abstract

The conserved eukaryotic THO-TREX complex acts at the interface between transcription and mRNA export and affects transcription-associated recombination. To investigate the interdependence of nuclear mRNA processes and their impact on genomic integrity, we analyzed transcript accumulation and recombination of 40 selected mutants covering representative steps of the biogenesis and export of the messenger ribonucleoprotein particle (mRNP). None of the mutants analyzed shared the strong transcript-accumulation defect and hyperrecombination of THO mutants. Nevertheless, mutants in 3' end cleavage/polyadenylation, nuclear exosome, and mRNA export showed a weak but significant effect on recombination and transcript accumulation. Mutants of the nuclear exosome (rrp6) and 3' end processing factors (rna14 and rna15) showed inefficient transcription elongation and genetic interactions with THO. The results suggest a tight interdependence among mRNP biogenesis steps and transcription and an unexpected effect of the nuclear exosome and the cleavage/polyadenylation factors on transcription elongation and genetic integrity.

Authors+Show Affiliations

Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15949445

Citation

Luna, Rosa, et al. "Interdependence Between Transcription and mRNP Processing and Export, and Its Impact On Genetic Stability." Molecular Cell, vol. 18, no. 6, 2005, pp. 711-22.
Luna R, Jimeno S, Marín M, et al. Interdependence between transcription and mRNP processing and export, and its impact on genetic stability. Mol Cell. 2005;18(6):711-22.
Luna, R., Jimeno, S., Marín, M., Huertas, P., García-Rubio, M., & Aguilera, A. (2005). Interdependence between transcription and mRNP processing and export, and its impact on genetic stability. Molecular Cell, 18(6), 711-22.
Luna R, et al. Interdependence Between Transcription and mRNP Processing and Export, and Its Impact On Genetic Stability. Mol Cell. 2005 Jun 10;18(6):711-22. PubMed PMID: 15949445.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interdependence between transcription and mRNP processing and export, and its impact on genetic stability. AU - Luna,Rosa, AU - Jimeno,Sonia, AU - Marín,Mercedes, AU - Huertas,Pablo, AU - García-Rubio,María, AU - Aguilera,Andrés, PY - 2004/12/28/received PY - 2005/03/22/revised PY - 2005/05/04/accepted PY - 2005/6/14/pubmed PY - 2005/7/29/medline PY - 2005/6/14/entrez SP - 711 EP - 22 JF - Molecular cell JO - Mol Cell VL - 18 IS - 6 N2 - The conserved eukaryotic THO-TREX complex acts at the interface between transcription and mRNA export and affects transcription-associated recombination. To investigate the interdependence of nuclear mRNA processes and their impact on genomic integrity, we analyzed transcript accumulation and recombination of 40 selected mutants covering representative steps of the biogenesis and export of the messenger ribonucleoprotein particle (mRNP). None of the mutants analyzed shared the strong transcript-accumulation defect and hyperrecombination of THO mutants. Nevertheless, mutants in 3' end cleavage/polyadenylation, nuclear exosome, and mRNA export showed a weak but significant effect on recombination and transcript accumulation. Mutants of the nuclear exosome (rrp6) and 3' end processing factors (rna14 and rna15) showed inefficient transcription elongation and genetic interactions with THO. The results suggest a tight interdependence among mRNP biogenesis steps and transcription and an unexpected effect of the nuclear exosome and the cleavage/polyadenylation factors on transcription elongation and genetic integrity. SN - 1097-2765 UR - https://www.unboundmedicine.com/medline/citation/15949445/Interdependence_between_transcription_and_mRNP_processing_and_export_and_its_impact_on_genetic_stability_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1097-2765(05)01307-9 DB - PRIME DP - Unbound Medicine ER -