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Lipopolysaccharide-binding protein is produced in the epididymis and associated with spermatozoa and prostasomes.
J Reprod Immunol. 2005 Jun; 66(1):33-43.JR

Abstract

Lipopolysaccharide-binding protein (LBP) is an acute phase protein known to play a central role in the defense against Gram-negative bacteria. It binds lipopolysaccharides of Gram-negative bacteria and, after binding to CD14, the complex signals through Toll-like receptor (TLR)-4, eliciting host-defense responses, such as cytokine production, in inflammatory cells. The present study demonstrates constitutive expression of the gene encoding lipopolysaccharide-binding protein in the epithelium of the human epididymis by in situ hybridization. Using immunohistochemistry lipopolysaccharide-binding protein was shown to be present in the same cells and also attached to the heads and tails of spermatozoa. Cell-free seminal plasma, lysed spermatozoa and lysed prostasomes were subjected to Western blot; all showed immunoreactive bands corresponding to the size of lipopolysaccharide-binding protein. Gel filtration demonstrated that lipopolysaccharide-binding protein colocalizes with prostasomes. The concentration of lipopolysacharide-binding protein in seminal plasma was 127+/-42ng/mL (mean+/-S.D.; range 73-215ng/mL). Taken together, our results suggest roles for lipopolysaccharide-binding protein during human reproduction.

Authors+Show Affiliations

Department of Clinical Chemistry, Malmö University Hospital, SE-205 02 Malmö, Sweden. johan.malm@klkemi.mas.lu.seNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15949560

Citation

Malm, Johan, et al. "Lipopolysaccharide-binding Protein Is Produced in the Epididymis and Associated With Spermatozoa and Prostasomes." Journal of Reproductive Immunology, vol. 66, no. 1, 2005, pp. 33-43.
Malm J, Nordahl EA, Bjartell A, et al. Lipopolysaccharide-binding protein is produced in the epididymis and associated with spermatozoa and prostasomes. J Reprod Immunol. 2005;66(1):33-43.
Malm, J., Nordahl, E. A., Bjartell, A., Sørensen, O. E., Frohm, B., Dentener, M. A., & Egesten, A. (2005). Lipopolysaccharide-binding protein is produced in the epididymis and associated with spermatozoa and prostasomes. Journal of Reproductive Immunology, 66(1), 33-43.
Malm J, et al. Lipopolysaccharide-binding Protein Is Produced in the Epididymis and Associated With Spermatozoa and Prostasomes. J Reprod Immunol. 2005;66(1):33-43. PubMed PMID: 15949560.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lipopolysaccharide-binding protein is produced in the epididymis and associated with spermatozoa and prostasomes. AU - Malm,Johan, AU - Nordahl,Emma Andersson, AU - Bjartell,Anders, AU - Sørensen,Ole E, AU - Frohm,Birgitta, AU - Dentener,Mieke A, AU - Egesten,Arne, PY - 2004/05/24/received PY - 2005/01/13/revised PY - 2005/01/25/accepted PY - 2005/6/14/pubmed PY - 2005/10/19/medline PY - 2005/6/14/entrez SP - 33 EP - 43 JF - Journal of reproductive immunology JO - J Reprod Immunol VL - 66 IS - 1 N2 - Lipopolysaccharide-binding protein (LBP) is an acute phase protein known to play a central role in the defense against Gram-negative bacteria. It binds lipopolysaccharides of Gram-negative bacteria and, after binding to CD14, the complex signals through Toll-like receptor (TLR)-4, eliciting host-defense responses, such as cytokine production, in inflammatory cells. The present study demonstrates constitutive expression of the gene encoding lipopolysaccharide-binding protein in the epithelium of the human epididymis by in situ hybridization. Using immunohistochemistry lipopolysaccharide-binding protein was shown to be present in the same cells and also attached to the heads and tails of spermatozoa. Cell-free seminal plasma, lysed spermatozoa and lysed prostasomes were subjected to Western blot; all showed immunoreactive bands corresponding to the size of lipopolysaccharide-binding protein. Gel filtration demonstrated that lipopolysaccharide-binding protein colocalizes with prostasomes. The concentration of lipopolysacharide-binding protein in seminal plasma was 127+/-42ng/mL (mean+/-S.D.; range 73-215ng/mL). Taken together, our results suggest roles for lipopolysaccharide-binding protein during human reproduction. SN - 0165-0378 UR - https://www.unboundmedicine.com/medline/citation/15949560/Lipopolysaccharide_binding_protein_is_produced_in_the_epididymis_and_associated_with_spermatozoa_and_prostasomes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165-0378(05)00022-7 DB - PRIME DP - Unbound Medicine ER -