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The pig eye as a novel model of glaucoma.
Exp Eye Res. 2005 Nov; 81(5):561-9.EE

Abstract

We validated the pig eye as a model of glaucoma, based on chronic elevation of intraocular pressure (IOP). IOP was elevated by cauterising three episcleral veins in each of the left eyes of five adult pigs. Right eyes were used as controls. Measurement of IOP was performed during the experiment with an applanation tonometer (Tono-Pen). Five months after episcleral vein occlusion, retinal ganglion cells (RGCs) from both cauterised and control eyes were retrogradely backfilled with Fluoro-Gold. Analysis of RGC loss and morphometric as characterization of surviving RGCs was performed using whole-mounted retinas. Elevation of IOP was apparent after three weeks of episcleral vein cauterisation and it remained elevated for at least 21 weeks (duration of the experiments). Analysis of RGC loss after chronic elevation of IOP revealed that RGC death was significant in the mid-peripheral and peripheral retina, mainly in the temporal quadrants of both retinal regions. Moreover the mean soma area of remaining RGCs was observed to increase and we found a greater loss of large RGCs in the mid-peripheral and peripheral retina. We conclude that the pattern of RGC death induced in the pig retina by episcleral vein cauterisation resembles that found in human glaucoma. On the basis of this study, the pig retina may be considered as a suitable model for glaucoma-related studies, based on its similarity with human and on its affordability.

Authors+Show Affiliations

Department of Cellular Biology, Faculty of Medicine, University of the Basque Country, E-48940 Leioa, Vizcaya, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15949799

Citation

Ruiz-Ederra, Javier, et al. "The Pig Eye as a Novel Model of Glaucoma." Experimental Eye Research, vol. 81, no. 5, 2005, pp. 561-9.
Ruiz-Ederra J, García M, Hernández M, et al. The pig eye as a novel model of glaucoma. Exp Eye Res. 2005;81(5):561-9.
Ruiz-Ederra, J., García, M., Hernández, M., Urcola, H., Hernández-Barbáchano, E., Araiz, J., & Vecino, E. (2005). The pig eye as a novel model of glaucoma. Experimental Eye Research, 81(5), 561-9.
Ruiz-Ederra J, et al. The Pig Eye as a Novel Model of Glaucoma. Exp Eye Res. 2005;81(5):561-9. PubMed PMID: 15949799.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The pig eye as a novel model of glaucoma. AU - Ruiz-Ederra,Javier, AU - García,Mónica, AU - Hernández,María, AU - Urcola,Haritz, AU - Hernández-Barbáchano,Ernesto, AU - Araiz,Javier, AU - Vecino,Elena, Y1 - 2005/06/09/ PY - 2004/11/17/received PY - 2005/03/15/revised PY - 2005/03/29/accepted PY - 2005/6/14/pubmed PY - 2006/1/24/medline PY - 2005/6/14/entrez SP - 561 EP - 9 JF - Experimental eye research JO - Exp Eye Res VL - 81 IS - 5 N2 - We validated the pig eye as a model of glaucoma, based on chronic elevation of intraocular pressure (IOP). IOP was elevated by cauterising three episcleral veins in each of the left eyes of five adult pigs. Right eyes were used as controls. Measurement of IOP was performed during the experiment with an applanation tonometer (Tono-Pen). Five months after episcleral vein occlusion, retinal ganglion cells (RGCs) from both cauterised and control eyes were retrogradely backfilled with Fluoro-Gold. Analysis of RGC loss and morphometric as characterization of surviving RGCs was performed using whole-mounted retinas. Elevation of IOP was apparent after three weeks of episcleral vein cauterisation and it remained elevated for at least 21 weeks (duration of the experiments). Analysis of RGC loss after chronic elevation of IOP revealed that RGC death was significant in the mid-peripheral and peripheral retina, mainly in the temporal quadrants of both retinal regions. Moreover the mean soma area of remaining RGCs was observed to increase and we found a greater loss of large RGCs in the mid-peripheral and peripheral retina. We conclude that the pattern of RGC death induced in the pig retina by episcleral vein cauterisation resembles that found in human glaucoma. On the basis of this study, the pig retina may be considered as a suitable model for glaucoma-related studies, based on its similarity with human and on its affordability. SN - 0014-4835 UR - https://www.unboundmedicine.com/medline/citation/15949799/The_pig_eye_as_a_novel_model_of_glaucoma_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4835(05)00106-5 DB - PRIME DP - Unbound Medicine ER -