Simultaneous quantification of stavudine, lamivudine and nevirapine by UV spectroscopy, reverse phase HPLC and HPTLC in tablets.J Pharm Biomed Anal. 2005 Sep 15; 39(3-4):801-4.JP
In the present study, simultaneous quantification of stavudine (SV), lamivudine (LV) and nevirapine (NV) in tablets by UV spectroscopy, reverse phase HPLC (RP-HPLC) and HPTLC methods were developed. In the UV multi-component spectral method, SV, LV and NV was quantified at 266, 271 and 315 nm, respectively. In the RP-HPLC method, the drugs were resolved using a mobile phase of 20 mM sodium phosphate buffer (containing 8 mM 1-octanesulphonicacid sodium salt):acetonitrile (4:1, v/v) with pH adjusted to 3.5 using phosphoric acid on a C18-ODS-Hypersil (5 microm, 250 mm x 4.6 mm) column in isocratic mode. The retention time of SV, LV and NV was 2.85, 4.33 and 8.39 min, respectively. In the HPTLC method, the chromatograms were developed using a mobile phase of chloroform:methanol (9:1, v/v) on precoated plate of silica gel 60 F254 and quantified by densitometric absorbance mode at 265 nm. The Rf of SV, LV and NV were 0.21-0.27, 0.62-0.72 and 0.82-0.93, respectively. Recovery values of 99.16-101.89%, percentage relative standard deviation of <0.7 and correlation coefficient (linear dynamic range) of 0.9843-0.9999 shows that the developed methods were accurate and precise. These methods can be employed for the routine analysis of tablets containing SV, LV and NV.
