Tags

Type your tag names separated by a space and hit enter

A nanoparticle amplification based quartz crystal microbalance DNA sensor for detection of Escherichia coli O157:H7.
Biosens Bioelectron. 2006 Jan 15; 21(7):1178-85.BB

Abstract

A quartz crystal microbalance (QCM) DNA sensor, based on the nanoparticle amplification method, was developed for detection of Escherichia coli O157:H7. A thiolated single-stranded DNA (ssDNA) probe specific to E. coli O157:H7 eaeA gene was immobilized onto the QCM sensor surface through self-assembly. The hybridization was induced by exposing the ssDNA probe to the complementary target DNA, and resulted in the mass change and therefore frequency change of the QCM. Streptavidin conjugated Fe(3)O(4) nanoparticles (average diameter=145 nm) were used as "mass enhancers" to amplify the frequency change. Synthesized biotinylated oligonucleotides as well as E. coli O157:H7 eaeA gene fragments (151 bases) amplified using asymmetric PCR with biotin labeled primers were tested. As low as 10(-12)M synthesized oligonucleotides and 2.67 x 10(2) colony forming unit (CFU)/ml E. coli O157:H7 cells can be detected by the sensor. Linear correlation between frequency change and logarithmic number of bacterial cell concentration was found for E. coli O157:H7 from 2.67 x 10(2) to 2.67 x 10(6)CFU/ml.

Authors+Show Affiliations

Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

15951163

Citation

Mao, Xiaole, et al. "A Nanoparticle Amplification Based Quartz Crystal Microbalance DNA Sensor for Detection of Escherichia Coli O157:H7." Biosensors & Bioelectronics, vol. 21, no. 7, 2006, pp. 1178-85.
Mao X, Yang L, Su XL, et al. A nanoparticle amplification based quartz crystal microbalance DNA sensor for detection of Escherichia coli O157:H7. Biosens Bioelectron. 2006;21(7):1178-85.
Mao, X., Yang, L., Su, X. L., & Li, Y. (2006). A nanoparticle amplification based quartz crystal microbalance DNA sensor for detection of Escherichia coli O157:H7. Biosensors & Bioelectronics, 21(7), 1178-85.
Mao X, et al. A Nanoparticle Amplification Based Quartz Crystal Microbalance DNA Sensor for Detection of Escherichia Coli O157:H7. Biosens Bioelectron. 2006 Jan 15;21(7):1178-85. PubMed PMID: 15951163.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A nanoparticle amplification based quartz crystal microbalance DNA sensor for detection of Escherichia coli O157:H7. AU - Mao,Xiaole, AU - Yang,Liju, AU - Su,Xiao-Li, AU - Li,Yanbin, Y1 - 2005/06/13/ PY - 2005/02/01/received PY - 2005/04/28/revised PY - 2005/04/29/accepted PY - 2005/6/14/pubmed PY - 2006/3/18/medline PY - 2005/6/14/entrez SP - 1178 EP - 85 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 21 IS - 7 N2 - A quartz crystal microbalance (QCM) DNA sensor, based on the nanoparticle amplification method, was developed for detection of Escherichia coli O157:H7. A thiolated single-stranded DNA (ssDNA) probe specific to E. coli O157:H7 eaeA gene was immobilized onto the QCM sensor surface through self-assembly. The hybridization was induced by exposing the ssDNA probe to the complementary target DNA, and resulted in the mass change and therefore frequency change of the QCM. Streptavidin conjugated Fe(3)O(4) nanoparticles (average diameter=145 nm) were used as "mass enhancers" to amplify the frequency change. Synthesized biotinylated oligonucleotides as well as E. coli O157:H7 eaeA gene fragments (151 bases) amplified using asymmetric PCR with biotin labeled primers were tested. As low as 10(-12)M synthesized oligonucleotides and 2.67 x 10(2) colony forming unit (CFU)/ml E. coli O157:H7 cells can be detected by the sensor. Linear correlation between frequency change and logarithmic number of bacterial cell concentration was found for E. coli O157:H7 from 2.67 x 10(2) to 2.67 x 10(6)CFU/ml. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/15951163/A_nanoparticle_amplification_based_quartz_crystal_microbalance_DNA_sensor_for_detection_of_Escherichia_coli_O157:H7_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(05)00134-X DB - PRIME DP - Unbound Medicine ER -