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Monitoring DNA damage in indigenous blue mussels (Mytilus edulis) sampled from coastal sites in Denmark.
Mutat Res. 2005 Aug 01; 585(1-2):33-42.MR

Abstract

Damage to DNA detected by use of the single-cell gel electrophoresis (comet) assay was monitored in blue mussels, Mytilus edulis, sampled from coastal waters in Denmark. Mussels from five locations in Køge Bay, an area receiving wastewater from many industries and municipalities, were collected five times during 1999 and six times in 2001. In 1999, both gill cells and haemolymph cells were examined, and sediments were sampled on three dates from the same five locations. In the autumn of 1999, mussels were also collected at six reference sites without known pollution. Results showed a significantly higher level of DNA damage in gill cells compared with haemolymph cells. Because of this, only gill cells were sampled for the monitoring in 2001. Levels of DNA damage, expressed as tail moments, were significantly higher for the mussels in Køge Bay when compared with levels of DNA damage in mussels from the non-polluted coastal areas. No clear seasonal variation was demonstrated. Analysis of the correlation between chromium, nickel, cadmium and mercury in sediments and tail moments in haemolymph and gill cells from the five sites showed a statistically significant positive correlation between tail moments and chromium, nickel and cadmium (P<0.01). The overall conclusion was that the comet assay on blue mussels could be useful for screening of genotoxic pollution in marine waters.

Authors+Show Affiliations

Department of Environment, Technology and Social Studies, Building 11.2, Roskilde University, DK-4000 Roskilde, Denmark. jr@ruc.dkNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15951227

Citation

Rank, Jette, et al. "Monitoring DNA Damage in Indigenous Blue Mussels (Mytilus Edulis) Sampled From Coastal Sites in Denmark." Mutation Research, vol. 585, no. 1-2, 2005, pp. 33-42.
Rank J, Jensen K, Jespersen PH. Monitoring DNA damage in indigenous blue mussels (Mytilus edulis) sampled from coastal sites in Denmark. Mutat Res. 2005;585(1-2):33-42.
Rank, J., Jensen, K., & Jespersen, P. H. (2005). Monitoring DNA damage in indigenous blue mussels (Mytilus edulis) sampled from coastal sites in Denmark. Mutation Research, 585(1-2), 33-42.
Rank J, Jensen K, Jespersen PH. Monitoring DNA Damage in Indigenous Blue Mussels (Mytilus Edulis) Sampled From Coastal Sites in Denmark. Mutat Res. 2005 Aug 1;585(1-2):33-42. PubMed PMID: 15951227.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Monitoring DNA damage in indigenous blue mussels (Mytilus edulis) sampled from coastal sites in Denmark. AU - Rank,Jette, AU - Jensen,Klara, AU - Jespersen,Per Homann, PY - 2004/03/16/received PY - 2005/03/07/revised PY - 2005/04/08/accepted PY - 2005/6/14/pubmed PY - 2005/9/2/medline PY - 2005/6/14/entrez SP - 33 EP - 42 JF - Mutation research JO - Mutat Res VL - 585 IS - 1-2 N2 - Damage to DNA detected by use of the single-cell gel electrophoresis (comet) assay was monitored in blue mussels, Mytilus edulis, sampled from coastal waters in Denmark. Mussels from five locations in Køge Bay, an area receiving wastewater from many industries and municipalities, were collected five times during 1999 and six times in 2001. In 1999, both gill cells and haemolymph cells were examined, and sediments were sampled on three dates from the same five locations. In the autumn of 1999, mussels were also collected at six reference sites without known pollution. Results showed a significantly higher level of DNA damage in gill cells compared with haemolymph cells. Because of this, only gill cells were sampled for the monitoring in 2001. Levels of DNA damage, expressed as tail moments, were significantly higher for the mussels in Køge Bay when compared with levels of DNA damage in mussels from the non-polluted coastal areas. No clear seasonal variation was demonstrated. Analysis of the correlation between chromium, nickel, cadmium and mercury in sediments and tail moments in haemolymph and gill cells from the five sites showed a statistically significant positive correlation between tail moments and chromium, nickel and cadmium (P<0.01). The overall conclusion was that the comet assay on blue mussels could be useful for screening of genotoxic pollution in marine waters. SN - 0027-5107 UR - https://www.unboundmedicine.com/medline/citation/15951227/Monitoring_DNA_damage_in_indigenous_blue_mussels__Mytilus_edulis__sampled_from_coastal_sites_in_Denmark_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1383-5718(05)00128-2 DB - PRIME DP - Unbound Medicine ER -