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Identification and characterization of an aquaporin 1 immunoreactive amacrine-type cell of the mouse retina.
J Comp Neurol 2005; 488(3):352-67JC

Abstract

Using immunocytochemistry, a type of amacrine cell that is immunoreactive for aquaporin 1 was identified in the mouse retina. AQP1 immunoreactivity was found in photoreceptor cells of the outer nuclear layer (ONL) and in a distinct type of amacrine cells of the inner nuclear layer (INL). AQP1-immunoreactive (IR) amacrine cell somata were located in the INL and their processes extended through strata 3 and 4 of the inner plexiform layer (IPL) with thin varicosities. The density of the AQP1-IR amacrine cells increased from 100/mm(2) in the peripheral retina to 350/mm(2) in the central retina. The AQP1-IR amacrine cells comprise 0.5% of the total amacrine cells. The AQP1-IR amacrine cell bodies formed a regular mosaic, which suggested that they represent a single type of amacrine cell. Double labeling with AQP1 and glycine, gamma-aminobutyric acid (GABA) or GAD(65) antiserum demonstrated that the AQP1-IR amacrine cells expressed GABA or GAD(65) but not glycine. Their synaptic input was primarily from other amacrine cell processes. They also received synaptic inputs from a few cone bipolar cells. The primary synaptic targets were ganglion cells, followed by other amacrine cells and cone bipolar cells. In addition, gap junctions between an AQP1-IR amacrine process and another amacrine process were rarely observed. In summary, a GABAergic amacrine cell type labeled by an antibody against AQP1 was identified in the mouse retina and was found to play a possible role in transferring a certain type of visual information from other amacrine or a few cone bipolar cells primarily to ganglion cells.

Authors+Show Affiliations

Department of Anatomy, College of Medicine, The Catholic University of Korea, Seoul 137-701, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15952169

Citation

Kang, Tae-Hoon, et al. "Identification and Characterization of an Aquaporin 1 Immunoreactive Amacrine-type Cell of the Mouse Retina." The Journal of Comparative Neurology, vol. 488, no. 3, 2005, pp. 352-67.
Kang TH, Choi YK, Kim IB, et al. Identification and characterization of an aquaporin 1 immunoreactive amacrine-type cell of the mouse retina. J Comp Neurol. 2005;488(3):352-67.
Kang, T. H., Choi, Y. K., Kim, I. B., Oh, S. J., & Chun, M. H. (2005). Identification and characterization of an aquaporin 1 immunoreactive amacrine-type cell of the mouse retina. The Journal of Comparative Neurology, 488(3), pp. 352-67.
Kang TH, et al. Identification and Characterization of an Aquaporin 1 Immunoreactive Amacrine-type Cell of the Mouse Retina. J Comp Neurol. 2005 Aug 1;488(3):352-67. PubMed PMID: 15952169.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and characterization of an aquaporin 1 immunoreactive amacrine-type cell of the mouse retina. AU - Kang,Tae-Hoon, AU - Choi,Yeon-Kyung, AU - Kim,In-Beom, AU - Oh,Su-Ja, AU - Chun,Myung-Hoon, PY - 2005/6/14/pubmed PY - 2005/11/1/medline PY - 2005/6/14/entrez SP - 352 EP - 67 JF - The Journal of comparative neurology JO - J. Comp. Neurol. VL - 488 IS - 3 N2 - Using immunocytochemistry, a type of amacrine cell that is immunoreactive for aquaporin 1 was identified in the mouse retina. AQP1 immunoreactivity was found in photoreceptor cells of the outer nuclear layer (ONL) and in a distinct type of amacrine cells of the inner nuclear layer (INL). AQP1-immunoreactive (IR) amacrine cell somata were located in the INL and their processes extended through strata 3 and 4 of the inner plexiform layer (IPL) with thin varicosities. The density of the AQP1-IR amacrine cells increased from 100/mm(2) in the peripheral retina to 350/mm(2) in the central retina. The AQP1-IR amacrine cells comprise 0.5% of the total amacrine cells. The AQP1-IR amacrine cell bodies formed a regular mosaic, which suggested that they represent a single type of amacrine cell. Double labeling with AQP1 and glycine, gamma-aminobutyric acid (GABA) or GAD(65) antiserum demonstrated that the AQP1-IR amacrine cells expressed GABA or GAD(65) but not glycine. Their synaptic input was primarily from other amacrine cell processes. They also received synaptic inputs from a few cone bipolar cells. The primary synaptic targets were ganglion cells, followed by other amacrine cells and cone bipolar cells. In addition, gap junctions between an AQP1-IR amacrine process and another amacrine process were rarely observed. In summary, a GABAergic amacrine cell type labeled by an antibody against AQP1 was identified in the mouse retina and was found to play a possible role in transferring a certain type of visual information from other amacrine or a few cone bipolar cells primarily to ganglion cells. SN - 0021-9967 UR - https://www.unboundmedicine.com/medline/citation/15952169/Identification_and_characterization_of_an_aquaporin_1_immunoreactive_amacrine_type_cell_of_the_mouse_retina_ L2 - https://doi.org/10.1002/cne.20589 DB - PRIME DP - Unbound Medicine ER -