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[Determination of catechins and caffeine in tea and tea beverages by high-performance liquid chromatography].
Wei Sheng Yan Jiu. 2005 Mar; 34(2):187-90.WS

Abstract

OBJECTIVE

A high-performance liquid chromatography method was developed for the determination of total tea catechin and caffeine.

METHODS

The catechins needed to be determined are: (-)-catechin, (-)-epigallocatechin, (-)-epigallocatechin gallate, (-)-epicatechin, (-)-epicatechin gallate, (-)-gallocatechin, (-)-gallocatechin gallate, (-)-catechin gallate and caffeine. The catechins in the tea was extracted with water:ethanol (3:7) by sonication for 20 min. After the extraction solution was centrifuged, it was analyzed by HPLC. The column used was a CAPCELL PAK C18 MG (4.6mm I.D. x 150mm) and gradient elution at constant column temperature with 30 degrees C under UV detector with 210nm. Mobile phase A was 0.1% H3PO4 in water, mobile phase B was 0.1% H3PO4 in methanol with the flow rate 1ml/min.

RESULTS

The results showed that the concentration of the catechins and caffeine and their peak areas achieved good linear relation, r > 0.999. The recoveries were between 61.7%-117.3%. RSD was below 10%. With this method, some tea samples were determined, the results were favorable.

CONCLUSION

The method can be applied for determination of catechin and caffeine in tea and tea beverages.

Authors+Show Affiliations

Ling Y
National Institute for Nutrition and Food Safety, Chinese Center for Diseases Control and Prevention, Beijing 100050, China.
Zhao YF
No affiliation info available
Li ZJ
No affiliation info available
Zhang G
No affiliation info available
Wu Y
No affiliation info available

MeSH

CaffeineCamellia sinensisCatechinChromatography, High Pressure LiquidSensitivity and SpecificityTea

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

15952660

Citation

Ling, Yun, et al. "[Determination of Catechins and Caffeine in Tea and Tea Beverages By High-performance Liquid Chromatography]." Wei Sheng Yan Jiu = Journal of Hygiene Research, vol. 34, no. 2, 2005, pp. 187-90.
Ling Y, Zhao YF, Li ZJ, et al. [Determination of catechins and caffeine in tea and tea beverages by high-performance liquid chromatography]. Wei Sheng Yan Jiu. 2005;34(2):187-90.
Ling, Y., Zhao, Y. F., Li, Z. J., Zhang, G., & Wu, Y. (2005). [Determination of catechins and caffeine in tea and tea beverages by high-performance liquid chromatography]. Wei Sheng Yan Jiu = Journal of Hygiene Research, 34(2), 187-90.
Ling Y, et al. [Determination of Catechins and Caffeine in Tea and Tea Beverages By High-performance Liquid Chromatography]. Wei Sheng Yan Jiu. 2005;34(2):187-90. PubMed PMID: 15952660.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Determination of catechins and caffeine in tea and tea beverages by high-performance liquid chromatography]. AU - Ling,Yun, AU - Zhao,Yun-feng, AU - Li,Zhi-jun, AU - Zhang,Gong, AU - Wu,Yongning, PY - 2005/6/15/pubmed PY - 2006/9/9/medline PY - 2005/6/15/entrez SP - 187 EP - 90 JF - Wei sheng yan jiu = Journal of hygiene research JO - Wei Sheng Yan Jiu VL - 34 IS - 2 N2 - OBJECTIVE: A high-performance liquid chromatography method was developed for the determination of total tea catechin and caffeine. METHODS: The catechins needed to be determined are: (-)-catechin, (-)-epigallocatechin, (-)-epigallocatechin gallate, (-)-epicatechin, (-)-epicatechin gallate, (-)-gallocatechin, (-)-gallocatechin gallate, (-)-catechin gallate and caffeine. The catechins in the tea was extracted with water:ethanol (3:7) by sonication for 20 min. After the extraction solution was centrifuged, it was analyzed by HPLC. The column used was a CAPCELL PAK C18 MG (4.6mm I.D. x 150mm) and gradient elution at constant column temperature with 30 degrees C under UV detector with 210nm. Mobile phase A was 0.1% H3PO4 in water, mobile phase B was 0.1% H3PO4 in methanol with the flow rate 1ml/min. RESULTS: The results showed that the concentration of the catechins and caffeine and their peak areas achieved good linear relation, r > 0.999. The recoveries were between 61.7%-117.3%. RSD was below 10%. With this method, some tea samples were determined, the results were favorable. CONCLUSION: The method can be applied for determination of catechin and caffeine in tea and tea beverages. SN - 1000-8020 UR - https://www.unboundmedicine.com/medline/citation/15952660/[Determination_of_catechins_and_caffeine_in_tea_and_tea_beverages_by_high_performance_liquid_chromatography]_ DB - PRIME DP - Unbound Medicine ER -