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[Determination of catechins and caffeine in tea and tea beverages by high-performance liquid chromatography].
Wei Sheng Yan Jiu. 2005 Mar; 34(2):187-90.WS

Abstract

OBJECTIVE

A high-performance liquid chromatography method was developed for the determination of total tea catechin and caffeine.

METHODS

The catechins needed to be determined are: (-)-catechin, (-)-epigallocatechin, (-)-epigallocatechin gallate, (-)-epicatechin, (-)-epicatechin gallate, (-)-gallocatechin, (-)-gallocatechin gallate, (-)-catechin gallate and caffeine. The catechins in the tea was extracted with water:ethanol (3:7) by sonication for 20 min. After the extraction solution was centrifuged, it was analyzed by HPLC. The column used was a CAPCELL PAK C18 MG (4.6mm I.D. x 150mm) and gradient elution at constant column temperature with 30 degrees C under UV detector with 210nm. Mobile phase A was 0.1% H3PO4 in water, mobile phase B was 0.1% H3PO4 in methanol with the flow rate 1ml/min.

RESULTS

The results showed that the concentration of the catechins and caffeine and their peak areas achieved good linear relation, r > 0.999. The recoveries were between 61.7%-117.3%. RSD was below 10%. With this method, some tea samples were determined, the results were favorable.

CONCLUSION

The method can be applied for determination of catechin and caffeine in tea and tea beverages.

Authors+Show Affiliations

National Institute for Nutrition and Food Safety, Chinese Center for Diseases Control and Prevention, Beijing 100050, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

15952660

Citation

Ling, Yun, et al. "[Determination of Catechins and Caffeine in Tea and Tea Beverages By High-performance Liquid Chromatography]." Wei Sheng Yan Jiu = Journal of Hygiene Research, vol. 34, no. 2, 2005, pp. 187-90.
Ling Y, Zhao YF, Li ZJ, et al. [Determination of catechins and caffeine in tea and tea beverages by high-performance liquid chromatography]. Wei Sheng Yan Jiu. 2005;34(2):187-90.
Ling, Y., Zhao, Y. F., Li, Z. J., Zhang, G., & Wu, Y. (2005). [Determination of catechins and caffeine in tea and tea beverages by high-performance liquid chromatography]. Wei Sheng Yan Jiu = Journal of Hygiene Research, 34(2), 187-90.
Ling Y, et al. [Determination of Catechins and Caffeine in Tea and Tea Beverages By High-performance Liquid Chromatography]. Wei Sheng Yan Jiu. 2005;34(2):187-90. PubMed PMID: 15952660.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Determination of catechins and caffeine in tea and tea beverages by high-performance liquid chromatography]. AU - Ling,Yun, AU - Zhao,Yun-feng, AU - Li,Zhi-jun, AU - Zhang,Gong, AU - Wu,Yongning, PY - 2005/6/15/pubmed PY - 2006/9/9/medline PY - 2005/6/15/entrez SP - 187 EP - 90 JF - Wei sheng yan jiu = Journal of hygiene research JO - Wei Sheng Yan Jiu VL - 34 IS - 2 N2 - OBJECTIVE: A high-performance liquid chromatography method was developed for the determination of total tea catechin and caffeine. METHODS: The catechins needed to be determined are: (-)-catechin, (-)-epigallocatechin, (-)-epigallocatechin gallate, (-)-epicatechin, (-)-epicatechin gallate, (-)-gallocatechin, (-)-gallocatechin gallate, (-)-catechin gallate and caffeine. The catechins in the tea was extracted with water:ethanol (3:7) by sonication for 20 min. After the extraction solution was centrifuged, it was analyzed by HPLC. The column used was a CAPCELL PAK C18 MG (4.6mm I.D. x 150mm) and gradient elution at constant column temperature with 30 degrees C under UV detector with 210nm. Mobile phase A was 0.1% H3PO4 in water, mobile phase B was 0.1% H3PO4 in methanol with the flow rate 1ml/min. RESULTS: The results showed that the concentration of the catechins and caffeine and their peak areas achieved good linear relation, r > 0.999. The recoveries were between 61.7%-117.3%. RSD was below 10%. With this method, some tea samples were determined, the results were favorable. CONCLUSION: The method can be applied for determination of catechin and caffeine in tea and tea beverages. SN - 1000-8020 UR - https://www.unboundmedicine.com/medline/citation/15952660/[Determination_of_catechins_and_caffeine_in_tea_and_tea_beverages_by_high_performance_liquid_chromatography]_ L2 - https://medlineplus.gov/caffeine.html DB - PRIME DP - Unbound Medicine ER -