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Effects of the tumour necrosis factor-alpha inhibitors pentoxifylline and thalidomide in short-term experimental oral mucositis in hamsters.
Eur J Oral Sci 2005; 113(3):210-7EJ

Abstract

Oral mucositis is a frequent side-effect of cancer therapy. A definitive method of prophylaxis or treatment is not yet available. As pentoxifylline (PTX) and thalidomide (TLD) have been shown to inhibit cytokine synthesis, we studied the effects of these cytokine inhibitors in an experimental oral mucositis model. Oral mucositis was induced in Golden hamsters by the administration of 5-fluorouracil (5-FU) followed by mechanical trauma of the cheek pouch. On days 4, 5, 10, 12, 14 and 16, lesions induced by 5-FU were examined macroscopically and microscopically, and the presence and intensity of hyperemia, erythema, edema, inflammatory cell infiltration, hemorrhagic areas, ulcers and abscesses were recorded. Saline (control), PTX (5, 15, 45 mg kg(-1)) or TLD (10, 30, 90 mg kg(-1)) were administered daily and animals were killed on day 10 for macroscopic and histological analysis and assay of myeloperoxidase (MPO) activity. Animals were weighed daily, and total and differential leukocyte counts were performed on peripheral blood. PTX and TLD were found to reduce the macroscopic and histological parameters of oral mucositis and MPO activity. PTX and TLD also reversed peripheral neutrophilia, but only PTX prevented weight loss. The results indicate a protective effect of PTX and TLD, suggesting an important role for tumour necrosis factor-alpha (TNF-alpha) in the pathophysiology of 5-FU induced-oral mucositis in hamsters.

Authors+Show Affiliations

Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Rua Coronel Nunes de Melo 1127, Rodolfo Teófilo-CEP 60430-270, Fortaleza, Ceará, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15953245

Citation

Lima, V, et al. "Effects of the Tumour Necrosis Factor-alpha Inhibitors Pentoxifylline and Thalidomide in Short-term Experimental Oral Mucositis in Hamsters." European Journal of Oral Sciences, vol. 113, no. 3, 2005, pp. 210-7.
Lima V, Brito GA, Cunha FQ, et al. Effects of the tumour necrosis factor-alpha inhibitors pentoxifylline and thalidomide in short-term experimental oral mucositis in hamsters. Eur J Oral Sci. 2005;113(3):210-7.
Lima, V., Brito, G. A., Cunha, F. Q., Rebouças, C. G., Falcão, B. A., Augusto, R. F., ... Ribeiro, R. A. (2005). Effects of the tumour necrosis factor-alpha inhibitors pentoxifylline and thalidomide in short-term experimental oral mucositis in hamsters. European Journal of Oral Sciences, 113(3), pp. 210-7.
Lima V, et al. Effects of the Tumour Necrosis Factor-alpha Inhibitors Pentoxifylline and Thalidomide in Short-term Experimental Oral Mucositis in Hamsters. Eur J Oral Sci. 2005;113(3):210-7. PubMed PMID: 15953245.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of the tumour necrosis factor-alpha inhibitors pentoxifylline and thalidomide in short-term experimental oral mucositis in hamsters. AU - Lima,V, AU - Brito,G A C, AU - Cunha,F Q, AU - Rebouças,C G, AU - Falcão,B A A, AU - Augusto,R F, AU - Souza,M L P, AU - Leitão,B T, AU - Ribeiro,R A, PY - 2005/6/15/pubmed PY - 2005/7/22/medline PY - 2005/6/15/entrez SP - 210 EP - 7 JF - European journal of oral sciences JO - Eur. J. Oral Sci. VL - 113 IS - 3 N2 - Oral mucositis is a frequent side-effect of cancer therapy. A definitive method of prophylaxis or treatment is not yet available. As pentoxifylline (PTX) and thalidomide (TLD) have been shown to inhibit cytokine synthesis, we studied the effects of these cytokine inhibitors in an experimental oral mucositis model. Oral mucositis was induced in Golden hamsters by the administration of 5-fluorouracil (5-FU) followed by mechanical trauma of the cheek pouch. On days 4, 5, 10, 12, 14 and 16, lesions induced by 5-FU were examined macroscopically and microscopically, and the presence and intensity of hyperemia, erythema, edema, inflammatory cell infiltration, hemorrhagic areas, ulcers and abscesses were recorded. Saline (control), PTX (5, 15, 45 mg kg(-1)) or TLD (10, 30, 90 mg kg(-1)) were administered daily and animals were killed on day 10 for macroscopic and histological analysis and assay of myeloperoxidase (MPO) activity. Animals were weighed daily, and total and differential leukocyte counts were performed on peripheral blood. PTX and TLD were found to reduce the macroscopic and histological parameters of oral mucositis and MPO activity. PTX and TLD also reversed peripheral neutrophilia, but only PTX prevented weight loss. The results indicate a protective effect of PTX and TLD, suggesting an important role for tumour necrosis factor-alpha (TNF-alpha) in the pathophysiology of 5-FU induced-oral mucositis in hamsters. SN - 0909-8836 UR - https://www.unboundmedicine.com/medline/citation/15953245/Effects_of_the_tumour_necrosis_factor_alpha_inhibitors_pentoxifylline_and_thalidomide_in_short_term_experimental_oral_mucositis_in_hamsters_ L2 - https://doi.org/10.1111/j.1600-0722.2005.00216.x DB - PRIME DP - Unbound Medicine ER -