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Expression of HSP47 in usual interstitial pneumonia and nonspecific interstitial pneumonia.
Respir Res. 2005 Jun 14; 6:57.RR

Abstract

BACKGROUND

Heat shock protein (HSP) 47, a collagen-specific molecular chaperone, is involved in the processing and/or secretion of procollagens, and its expression is increased in various fibrotic diseases. The aim of this study was to determine whether quantitative immunohistochemical evaluation of the expression levels of HSP47, type I procollagen and alpha-smooth muscle actin (SMA) allows the differentiation of idiopathic usual interstitial pneumonia (UIP) from UIP associated with collagen vascular disease (CVD) and idiopathic nonspecific interstitial pneumonia (NSIP).

METHODS

We reviewed surgical lung biopsy specimens of 19 patients with idiopathic UIP, 7 with CVD-associated UIP and 16 with idiopathic NSIP and assigned a score for the expression of HSP47, type I procollagen and alpha-SMA in type II pneumocytes and/or lung fibroblasts (score 0 = no; 1 = weak; 2 = moderate; 3 = strong staining).

RESULTS

The expression level of HSP47 in type II pneumocytes of idiopathic UIP was significantly higher than in CVD-associated UIP and idiopathic NSIP. The expression of HSP47 in fibroblasts was significantly higher in idiopathic UIP and idiopathic NSIP than in CVD-associated UIP. The expression of type I procollagen in type II pneumocytes was significantly higher in idiopathic UIP than in idiopathic NSIP. The expression of type I procollagen in fibroblasts was not different in the three groups, while the expression of alpha-SMA in fibroblasts was significantly higher in idiopathic UIP than in idiopathic NSIP.

CONCLUSION

Our results suggest the existence of different fibrotic pathways among these groups involved in the expression of HSP47 and type I procollagen.

Authors+Show Affiliations

Second Department of Internal Medicine, Nagasaki University School of Medicine, Nagasaki, Japan. kakugawa@jasmine.ocn.ne.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Controlled Clinical Trial
Journal Article

Language

eng

PubMed ID

15955241

Citation

Kakugawa, Tomoyuki, et al. "Expression of HSP47 in Usual Interstitial Pneumonia and Nonspecific Interstitial Pneumonia." Respiratory Research, vol. 6, 2005, p. 57.
Kakugawa T, Mukae H, Hayashi T, et al. Expression of HSP47 in usual interstitial pneumonia and nonspecific interstitial pneumonia. Respir Res. 2005;6:57.
Kakugawa, T., Mukae, H., Hayashi, T., Ishii, H., Nakayama, S., Sakamoto, N., Yoshioka, S., Sugiyama, K., Mine, M., Mizuta, Y., & Kohno, S. (2005). Expression of HSP47 in usual interstitial pneumonia and nonspecific interstitial pneumonia. Respiratory Research, 6, 57.
Kakugawa T, et al. Expression of HSP47 in Usual Interstitial Pneumonia and Nonspecific Interstitial Pneumonia. Respir Res. 2005 Jun 14;6:57. PubMed PMID: 15955241.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of HSP47 in usual interstitial pneumonia and nonspecific interstitial pneumonia. AU - Kakugawa,Tomoyuki, AU - Mukae,Hiroshi, AU - Hayashi,Tomayoshi, AU - Ishii,Hiroshi, AU - Nakayama,Seiko, AU - Sakamoto,Noriho, AU - Yoshioka,Sumako, AU - Sugiyama,Kanako, AU - Mine,Mariko, AU - Mizuta,Yohei, AU - Kohno,Shigeru, Y1 - 2005/06/14/ PY - 2005/02/08/received PY - 2005/06/14/accepted PY - 2005/6/16/pubmed PY - 2006/3/18/medline PY - 2005/6/16/entrez SP - 57 EP - 57 JF - Respiratory research JO - Respir Res VL - 6 N2 - BACKGROUND: Heat shock protein (HSP) 47, a collagen-specific molecular chaperone, is involved in the processing and/or secretion of procollagens, and its expression is increased in various fibrotic diseases. The aim of this study was to determine whether quantitative immunohistochemical evaluation of the expression levels of HSP47, type I procollagen and alpha-smooth muscle actin (SMA) allows the differentiation of idiopathic usual interstitial pneumonia (UIP) from UIP associated with collagen vascular disease (CVD) and idiopathic nonspecific interstitial pneumonia (NSIP). METHODS: We reviewed surgical lung biopsy specimens of 19 patients with idiopathic UIP, 7 with CVD-associated UIP and 16 with idiopathic NSIP and assigned a score for the expression of HSP47, type I procollagen and alpha-SMA in type II pneumocytes and/or lung fibroblasts (score 0 = no; 1 = weak; 2 = moderate; 3 = strong staining). RESULTS: The expression level of HSP47 in type II pneumocytes of idiopathic UIP was significantly higher than in CVD-associated UIP and idiopathic NSIP. The expression of HSP47 in fibroblasts was significantly higher in idiopathic UIP and idiopathic NSIP than in CVD-associated UIP. The expression of type I procollagen in type II pneumocytes was significantly higher in idiopathic UIP than in idiopathic NSIP. The expression of type I procollagen in fibroblasts was not different in the three groups, while the expression of alpha-SMA in fibroblasts was significantly higher in idiopathic UIP than in idiopathic NSIP. CONCLUSION: Our results suggest the existence of different fibrotic pathways among these groups involved in the expression of HSP47 and type I procollagen. SN - 1465-993X UR - https://www.unboundmedicine.com/medline/citation/15955241/Expression_of_HSP47_in_usual_interstitial_pneumonia_and_nonspecific_interstitial_pneumonia_ L2 - https://respiratory-research.biomedcentral.com/articles/10.1186/1465-9921-6-57 DB - PRIME DP - Unbound Medicine ER -