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Cocaine pre-exposure enhances CRF-induced expression of c-fos mRNA in the central nucleus of the amygdala: an effect that parallels the effects of cocaine pre-exposure on CRF-induced locomotor activity.
Neurosci Lett. 2005 Aug 05; 383(3):209-14.NL

Abstract

There is evidence that cocaine pre-exposure produces changes in the responsivity of central corticotropin-releasing factor (CRF) systems and that these systems mediate some of the drug-related behavioural effects of acute stressors. The present experiment was conducted to assess the effects of repeated cocaine exposure on CRF-induced neuronal activation within two regions of the extended amygdala, the central nucleus of the amygdala (CeA) and lateral bed nucleus of the stria terminalis (BNST). In addition, CRF-induced neuronal activation was compared with CRF-induced locomotor activity. Rats were injected for 7 days with cocaine (days 1 and 7 in test chambers; days 2-6 in homecages) or saline. After 10 drug-free days, locomotor responsiveness to intracerebroventricular (i.c.v.) injections of CRF and Vehicle was assessed over 2-h test periods. Twenty-four to 48 h following testing for locomotor activity, animals were injected with either CRF or Vehicle, 30 min before being sacrificed. Subsequently, the brains were processed by in situ hybridization for c-fos mRNA, a widely used marker of neuronal activation, in the CeA and BNST. In CeA, i.c.v. CRF enhanced the expression of c-fos mRNA in cocaine, but not saline, pre-exposed animals; in the same animals, i.c.v. CRF resulted in enhanced locomotor activity in cocaine, but not saline, pre-exposed animals. The results demonstrate that repeated exposure to cocaine changes the neuronal response to CRF in the CeA; furthermore, they suggest that these changes in the CeA could potentially be of functional significance in the effects of repeated cocaine exposure on CRF-induced locomotor activity.

Authors+Show Affiliations

Centre for the Neurobiology of Stress, Departments of Life Science and Psychology, University of Toronto at Scarborough, 1265 Military Trail, Toronto, Ont., Canada M1C 1A4. erb@utsc.utoronto.caNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

15955413

Citation

Erb, Suzanne, et al. "Cocaine Pre-exposure Enhances CRF-induced Expression of C-fos mRNA in the Central Nucleus of the Amygdala: an Effect That Parallels the Effects of Cocaine Pre-exposure On CRF-induced Locomotor Activity." Neuroscience Letters, vol. 383, no. 3, 2005, pp. 209-14.
Erb S, Funk D, Lê AD. Cocaine pre-exposure enhances CRF-induced expression of c-fos mRNA in the central nucleus of the amygdala: an effect that parallels the effects of cocaine pre-exposure on CRF-induced locomotor activity. Neurosci Lett. 2005;383(3):209-14.
Erb, S., Funk, D., & Lê, A. D. (2005). Cocaine pre-exposure enhances CRF-induced expression of c-fos mRNA in the central nucleus of the amygdala: an effect that parallels the effects of cocaine pre-exposure on CRF-induced locomotor activity. Neuroscience Letters, 383(3), 209-14.
Erb S, Funk D, Lê AD. Cocaine Pre-exposure Enhances CRF-induced Expression of C-fos mRNA in the Central Nucleus of the Amygdala: an Effect That Parallels the Effects of Cocaine Pre-exposure On CRF-induced Locomotor Activity. Neurosci Lett. 2005 Aug 5;383(3):209-14. PubMed PMID: 15955413.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cocaine pre-exposure enhances CRF-induced expression of c-fos mRNA in the central nucleus of the amygdala: an effect that parallels the effects of cocaine pre-exposure on CRF-induced locomotor activity. AU - Erb,Suzanne, AU - Funk,Douglas, AU - Lê,Anh Dzung, Y1 - 2005/04/26/ PY - 2005/02/09/received PY - 2005/03/28/revised PY - 2005/04/04/accepted PY - 2005/6/16/pubmed PY - 2005/8/10/medline PY - 2005/6/16/entrez SP - 209 EP - 14 JF - Neuroscience letters JO - Neurosci Lett VL - 383 IS - 3 N2 - There is evidence that cocaine pre-exposure produces changes in the responsivity of central corticotropin-releasing factor (CRF) systems and that these systems mediate some of the drug-related behavioural effects of acute stressors. The present experiment was conducted to assess the effects of repeated cocaine exposure on CRF-induced neuronal activation within two regions of the extended amygdala, the central nucleus of the amygdala (CeA) and lateral bed nucleus of the stria terminalis (BNST). In addition, CRF-induced neuronal activation was compared with CRF-induced locomotor activity. Rats were injected for 7 days with cocaine (days 1 and 7 in test chambers; days 2-6 in homecages) or saline. After 10 drug-free days, locomotor responsiveness to intracerebroventricular (i.c.v.) injections of CRF and Vehicle was assessed over 2-h test periods. Twenty-four to 48 h following testing for locomotor activity, animals were injected with either CRF or Vehicle, 30 min before being sacrificed. Subsequently, the brains were processed by in situ hybridization for c-fos mRNA, a widely used marker of neuronal activation, in the CeA and BNST. In CeA, i.c.v. CRF enhanced the expression of c-fos mRNA in cocaine, but not saline, pre-exposed animals; in the same animals, i.c.v. CRF resulted in enhanced locomotor activity in cocaine, but not saline, pre-exposed animals. The results demonstrate that repeated exposure to cocaine changes the neuronal response to CRF in the CeA; furthermore, they suggest that these changes in the CeA could potentially be of functional significance in the effects of repeated cocaine exposure on CRF-induced locomotor activity. SN - 0304-3940 UR - https://www.unboundmedicine.com/medline/citation/15955413/Cocaine_pre_exposure_enhances_CRF_induced_expression_of_c_fos_mRNA_in_the_central_nucleus_of_the_amygdala:_an_effect_that_parallels_the_effects_of_cocaine_pre_exposure_on_CRF_induced_locomotor_activity_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3940(05)00398-8 DB - PRIME DP - Unbound Medicine ER -