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Effect of Aroclor 1254 on Sertoli cellular antioxidant system, androgen binding protein and lactate in adult rat in vitro.
Toxicology. 2005 Sep 01; 212(2-3):195-205.T

Abstract

Polychlorinated biphenyls (PCBs) are persistent and bioaccumulative environmental toxicants. Previous studies suggested that PCBs (Aroclor 1254) induce toxic effects including reproductive toxicity. The present study was designed to investigate the impact of Aroclor 1254 on Sertoli cellular function and antioxidant system of adult rat in vitro. Sertoli cells were isolated from adult rat testes and treated with various concentrations (10(-10) to 10(-7) M) of Aroclor 1254 for 6, 12 and 24 h. After the treatment period, cell viability was assessed and the Sertoli cellular antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), gamma-glutamyl transpeptidase (gamma-GT) and glutathione reductase (GR) and lipid peroxidation (LPO) were assayed. In addition, androgen binding protein (ABP) and lactate secretions were also quantified in Sertoli cell culture medium. Sertoli cellular viability and activity of antioxidant enzymes were significantly reduced in Aroclor 1254 (10(-10) to 10(-7) M) treatment for 6, 12 and 24 h whereas, the Sertoli cellular lipid peroxidation was significantly increased in a dose and duration dependent manner. In addition, ABP secretion diminished and lactate secretion was significantly elevated in the same manner. To conclude, the present study suggested that Aroclor 1254 disrupts Sertoli cellular metabolic functions such as ABP, lactate secretions and activity of antioxidant enzymes.

Authors+Show Affiliations

Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Taramani, Chennai 600113, Tamil Nadu, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15955608

Citation

Krishnamoorthy, G, et al. "Effect of Aroclor 1254 On Sertoli Cellular Antioxidant System, Androgen Binding Protein and Lactate in Adult Rat in Vitro." Toxicology, vol. 212, no. 2-3, 2005, pp. 195-205.
Krishnamoorthy G, Murugesan P, Muthuvel R, et al. Effect of Aroclor 1254 on Sertoli cellular antioxidant system, androgen binding protein and lactate in adult rat in vitro. Toxicology. 2005;212(2-3):195-205.
Krishnamoorthy, G., Murugesan, P., Muthuvel, R., Gunadharini, D. N., Vijayababu, M. R., Arunkumar, A., Venkataraman, P., Aruldhas, M. M., & Arunakaran, J. (2005). Effect of Aroclor 1254 on Sertoli cellular antioxidant system, androgen binding protein and lactate in adult rat in vitro. Toxicology, 212(2-3), 195-205.
Krishnamoorthy G, et al. Effect of Aroclor 1254 On Sertoli Cellular Antioxidant System, Androgen Binding Protein and Lactate in Adult Rat in Vitro. Toxicology. 2005 Sep 1;212(2-3):195-205. PubMed PMID: 15955608.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of Aroclor 1254 on Sertoli cellular antioxidant system, androgen binding protein and lactate in adult rat in vitro. AU - Krishnamoorthy,G, AU - Murugesan,P, AU - Muthuvel,R, AU - Gunadharini,D N, AU - Vijayababu,M R, AU - Arunkumar,A, AU - Venkataraman,P, AU - Aruldhas,M M, AU - Arunakaran,J, PY - 2005/03/08/received PY - 2005/04/26/revised PY - 2005/04/26/accepted PY - 2005/6/16/pubmed PY - 2005/9/17/medline PY - 2005/6/16/entrez SP - 195 EP - 205 JF - Toxicology JO - Toxicology VL - 212 IS - 2-3 N2 - Polychlorinated biphenyls (PCBs) are persistent and bioaccumulative environmental toxicants. Previous studies suggested that PCBs (Aroclor 1254) induce toxic effects including reproductive toxicity. The present study was designed to investigate the impact of Aroclor 1254 on Sertoli cellular function and antioxidant system of adult rat in vitro. Sertoli cells were isolated from adult rat testes and treated with various concentrations (10(-10) to 10(-7) M) of Aroclor 1254 for 6, 12 and 24 h. After the treatment period, cell viability was assessed and the Sertoli cellular antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), gamma-glutamyl transpeptidase (gamma-GT) and glutathione reductase (GR) and lipid peroxidation (LPO) were assayed. In addition, androgen binding protein (ABP) and lactate secretions were also quantified in Sertoli cell culture medium. Sertoli cellular viability and activity of antioxidant enzymes were significantly reduced in Aroclor 1254 (10(-10) to 10(-7) M) treatment for 6, 12 and 24 h whereas, the Sertoli cellular lipid peroxidation was significantly increased in a dose and duration dependent manner. In addition, ABP secretion diminished and lactate secretion was significantly elevated in the same manner. To conclude, the present study suggested that Aroclor 1254 disrupts Sertoli cellular metabolic functions such as ABP, lactate secretions and activity of antioxidant enzymes. SN - 0300-483X UR - https://www.unboundmedicine.com/medline/citation/15955608/Effect_of_Aroclor_1254_on_Sertoli_cellular_antioxidant_system_androgen_binding_protein_and_lactate_in_adult_rat_in_vitro_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0300-483X(05)00205-2 DB - PRIME DP - Unbound Medicine ER -