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Accumulation of CXCR3-expressing eosinophils and increased concentration of its ligands (IP10 and Mig) in bronchoalveolar lavage fluid of patients with chronic eosinophilic pneumonia.
Int Arch Allergy Immunol. 2005 Jul; 137(3):229-35.IA

Abstract

BACKGROUND

Since human peripheral eosinophils have been shown to migrate to the CXC chemokine receptor 3 (CXCR3) ligands IFN-gamma-inducible protein 10 (IP10) and monokine induced by IFN-gamma (Mig), this confirms that CXCR3 is functionally expressed on these cells. IP10 expression has been shown to be increased in the airways of asthmatics. Eosinophil accumulations are found in bronchoalveolar lavage fluid (BALF) from patients with chronic eosinophilic pneumonia (CEP). To examine the contribution of IP10 and Mig in the pathogenesis of CEP, we measured the concentration of IP10 and Mig, and evaluated the expression of CXCR3 on eosinophils in BALF taken from patients with CEP.

METHODS

The concentrations of IP10 and Mig in BALF were measured by ELISA. The proportion of CXCR3-expressing CD4+ T cells and CD16-negative eosinophils was determined by flow cytometry.

RESULTS

The BALF concentrations of IP10 and Mig were higher in patients with CEP, as well as in patients with sarcoidosis, when compared to healthy controls. The absolute number of CXCR3+ CD4+ T cells was significantly higher in the BALF of patients with sarcoidosis, but not in the patients with CEP, when compared to healthy volunteers. There were higher percentages of CXCR3-expressing eosinophils in the BALF than in the peripheral blood of patients with CEP.

CONCLUSIONS

Our findings suggest that IP10 and Mig contribute to the accumulation of CXCR3-expressing eosinophils in the lungs of patients with CEP, and modulate the eosinophilic inflammation of the lung.

Authors+Show Affiliations

Department of Cell Regulation, School of Medicine, Kagawa University, Kita-gun, Kagawa, Japan. kshigeki@med.kagawa-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15956791

Citation

Katoh, Shigeki, et al. "Accumulation of CXCR3-expressing Eosinophils and Increased Concentration of Its Ligands (IP10 and Mig) in Bronchoalveolar Lavage Fluid of Patients With Chronic Eosinophilic Pneumonia." International Archives of Allergy and Immunology, vol. 137, no. 3, 2005, pp. 229-35.
Katoh S, Fukushima K, Matsumoto N, et al. Accumulation of CXCR3-expressing eosinophils and increased concentration of its ligands (IP10 and Mig) in bronchoalveolar lavage fluid of patients with chronic eosinophilic pneumonia. Int Arch Allergy Immunol. 2005;137(3):229-35.
Katoh, S., Fukushima, K., Matsumoto, N., Ehara, N., Matsumoto, K., Yamauchi, A., & Hirashima, M. (2005). Accumulation of CXCR3-expressing eosinophils and increased concentration of its ligands (IP10 and Mig) in bronchoalveolar lavage fluid of patients with chronic eosinophilic pneumonia. International Archives of Allergy and Immunology, 137(3), 229-35.
Katoh S, et al. Accumulation of CXCR3-expressing Eosinophils and Increased Concentration of Its Ligands (IP10 and Mig) in Bronchoalveolar Lavage Fluid of Patients With Chronic Eosinophilic Pneumonia. Int Arch Allergy Immunol. 2005;137(3):229-35. PubMed PMID: 15956791.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Accumulation of CXCR3-expressing eosinophils and increased concentration of its ligands (IP10 and Mig) in bronchoalveolar lavage fluid of patients with chronic eosinophilic pneumonia. AU - Katoh,Shigeki, AU - Fukushima,Kiyoyasu, AU - Matsumoto,Nobuhiro, AU - Ehara,Naomi, AU - Matsumoto,Kiyoshi, AU - Yamauchi,Akira, AU - Hirashima,Mitsuomi, Y1 - 2005/06/13/ PY - 2004/08/20/received PY - 2005/02/21/accepted PY - 2005/6/16/pubmed PY - 2005/8/10/medline PY - 2005/6/16/entrez SP - 229 EP - 35 JF - International archives of allergy and immunology JO - Int Arch Allergy Immunol VL - 137 IS - 3 N2 - BACKGROUND: Since human peripheral eosinophils have been shown to migrate to the CXC chemokine receptor 3 (CXCR3) ligands IFN-gamma-inducible protein 10 (IP10) and monokine induced by IFN-gamma (Mig), this confirms that CXCR3 is functionally expressed on these cells. IP10 expression has been shown to be increased in the airways of asthmatics. Eosinophil accumulations are found in bronchoalveolar lavage fluid (BALF) from patients with chronic eosinophilic pneumonia (CEP). To examine the contribution of IP10 and Mig in the pathogenesis of CEP, we measured the concentration of IP10 and Mig, and evaluated the expression of CXCR3 on eosinophils in BALF taken from patients with CEP. METHODS: The concentrations of IP10 and Mig in BALF were measured by ELISA. The proportion of CXCR3-expressing CD4+ T cells and CD16-negative eosinophils was determined by flow cytometry. RESULTS: The BALF concentrations of IP10 and Mig were higher in patients with CEP, as well as in patients with sarcoidosis, when compared to healthy controls. The absolute number of CXCR3+ CD4+ T cells was significantly higher in the BALF of patients with sarcoidosis, but not in the patients with CEP, when compared to healthy volunteers. There were higher percentages of CXCR3-expressing eosinophils in the BALF than in the peripheral blood of patients with CEP. CONCLUSIONS: Our findings suggest that IP10 and Mig contribute to the accumulation of CXCR3-expressing eosinophils in the lungs of patients with CEP, and modulate the eosinophilic inflammation of the lung. SN - 1018-2438 UR - https://www.unboundmedicine.com/medline/citation/15956791/Accumulation_of_CXCR3_expressing_eosinophils_and_increased_concentration_of_its_ligands__IP10_and_Mig__in_bronchoalveolar_lavage_fluid_of_patients_with_chronic_eosinophilic_pneumonia_ L2 - https://www.karger.com?DOI=10.1159/000086335 DB - PRIME DP - Unbound Medicine ER -