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Analysis of larval antigens of Oestrus ovis for the diagnosis of oestrosis by enzyme-linked immunosorbent assay.
Med Vet Entomol. 2005 Jun; 19(2):151-7.MV

Abstract

A serodiagnostic test for the diagnosis of infestation by the sheep nasal bot fly, Oestrus ovis (Linné) was examined. The enzyme-linked immunosorbent assay (ELISA) technique was used to analyze and compare the production of immunoglobulin G (IgG) antibodies against excretory-secretory products (ESP) and crude extract (CE) antigens from all the different larval stages of O. ovis in the sera of 276 adult sheep sampled in summer (n = 135) and winter (n = 141). ESP from first stage larvae was the most sensitive, coating antigen in winter and ESP from second stage larvae during summer. The most specific values were obtained by ESP against L1 in winter and by CE against L3 in summer. These results show that the stage of larval development has a significant impact on the humoral immune response over the course of a season. A significant correlation (P < 0.001) was found between the number of O. ovis larvae and the serum antibody levels using all differents antigens, except L3 CE. In Spain, where a long favourable period exists for the evolution and development of the different stage larvae between March and November, the ELISA test using L1 ESP antigen during winter and L2 ESP antigen in summer may be used for ovine oestrosis immunodiagnosis.

Authors+Show Affiliations

Alcaide M
Department of Medicine and Animal Health, Faculty of Veterinary Sciences, University of Extremadura, Spain. malcaide@unex.es
Reina D
No affiliation info available
Frontera E
No affiliation info available
Navarrete I
No affiliation info available

MeSH

AnimalsAntigensDipteraEnzyme-Linked Immunosorbent AssayFemaleLarvaMyiasisSeasonsSheepSheep Diseases

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15958024

Citation

Alcaide, M, et al. "Analysis of Larval Antigens of Oestrus Ovis for the Diagnosis of Oestrosis By Enzyme-linked Immunosorbent Assay." Medical and Veterinary Entomology, vol. 19, no. 2, 2005, pp. 151-7.
Alcaide M, Reina D, Frontera E, et al. Analysis of larval antigens of Oestrus ovis for the diagnosis of oestrosis by enzyme-linked immunosorbent assay. Med Vet Entomol. 2005;19(2):151-7.
Alcaide, M., Reina, D., Frontera, E., & Navarrete, I. (2005). Analysis of larval antigens of Oestrus ovis for the diagnosis of oestrosis by enzyme-linked immunosorbent assay. Medical and Veterinary Entomology, 19(2), 151-7.
Alcaide M, et al. Analysis of Larval Antigens of Oestrus Ovis for the Diagnosis of Oestrosis By Enzyme-linked Immunosorbent Assay. Med Vet Entomol. 2005;19(2):151-7. PubMed PMID: 15958024.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of larval antigens of Oestrus ovis for the diagnosis of oestrosis by enzyme-linked immunosorbent assay. AU - Alcaide,M, AU - Reina,D, AU - Frontera,E, AU - Navarrete,I, PY - 2005/6/17/pubmed PY - 2005/7/12/medline PY - 2005/6/17/entrez SP - 151 EP - 7 JF - Medical and veterinary entomology JO - Med Vet Entomol VL - 19 IS - 2 N2 - A serodiagnostic test for the diagnosis of infestation by the sheep nasal bot fly, Oestrus ovis (Linné) was examined. The enzyme-linked immunosorbent assay (ELISA) technique was used to analyze and compare the production of immunoglobulin G (IgG) antibodies against excretory-secretory products (ESP) and crude extract (CE) antigens from all the different larval stages of O. ovis in the sera of 276 adult sheep sampled in summer (n = 135) and winter (n = 141). ESP from first stage larvae was the most sensitive, coating antigen in winter and ESP from second stage larvae during summer. The most specific values were obtained by ESP against L1 in winter and by CE against L3 in summer. These results show that the stage of larval development has a significant impact on the humoral immune response over the course of a season. A significant correlation (P < 0.001) was found between the number of O. ovis larvae and the serum antibody levels using all differents antigens, except L3 CE. In Spain, where a long favourable period exists for the evolution and development of the different stage larvae between March and November, the ELISA test using L1 ESP antigen during winter and L2 ESP antigen in summer may be used for ovine oestrosis immunodiagnosis. SN - 0269-283X UR - https://www.unboundmedicine.com/medline/citation/15958024/Analysis_of_larval_antigens_of_Oestrus_ovis_for_the_diagnosis_of_oestrosis_by_enzyme_linked_immunosorbent_assay_ DB - PRIME DP - Unbound Medicine ER -