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[Chondrocyte apoptosis and the expression of Bcl-2, Bax, Fas and iNos in articular cartilage in Kashin-Beck disease].
Di Yi Jun Yi Da Xue Xue Bao. 2005 Jun; 25(6):643-6.DY

Abstract

OBJECTIVE

To investigate the characteristics of chondrocyte apoptosis and distribution of Bcl-2, Bax, Fas and iNos expressions in articular cartilage in Kashin-Beck disease (KBD).

METHODS

Samples of articular cartilage were collected from 15 healthy children and 15 children with KBD diagnosed according to the Pathological Criteria of KBD Diagnosis in China. Chondrocyte apoptosis was detected by TUNEL method, and the articular chondrocytes positive for Bcl-2, Bax, Fas and iNos were stained by B-SA immunohistochemistry.

RESULTS

The percentage of apoptotic chondrocytes positively stained by TUNEL in the middle layer of articular cartilage was significantly higher in KBD children than in the control group (33.60%+/-2.71% vs 1.33%+/-0.41%, t=11.59, P<0.01). Significant difference in Bcl-2, Bax, Fas and iNos expressions was observed between the upper, middle and deep layers of the articular cartilage of KBD children (F =73.49-114.42, P<0.01), and staining for Bcl-2, Bax, Fas and iNos in KBD children was prominent in the upper layer (41.93%+/-12.26%, 45.60%+/-15.78%, 53.60%+/-16.49%, and 45.47%+/-14.02%, respectively) and the middle layer (14.93%+/-3.50%, 13.87%+/-4.32%, 23.27%+/-4.83%, and 21.67%+/-6.82%, respectively) of the articular cartilage; the percentages of chondrocytes positively stained for Bcl-2, Bax, Fas and iNos were significantly higher than those of the control group (t=11.75-18.65, P<0.01).

CONCLUSION

The percentages of apoptotic chondrocytes and chondrocytes positive for Bcl-2, Bax, Fas and iNos in the articular cartilage of children with KBD are significantly higher than those in healthy children.

Authors+Show Affiliations

Key Laboratory of Environment and Genes Related Diseases, Institute of Environment and Endemic Diseases of State Ministry of Education, Xi'an Jiaotong University, Xi'an 710061, China. sxjaner@21cn.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

15958298

Citation

Wang, Shi-jie, et al. "[Chondrocyte Apoptosis and the Expression of Bcl-2, Bax, Fas and iNos in Articular Cartilage in Kashin-Beck Disease]." Di 1 Jun Yi Da Xue Xue Bao = Academic Journal of the First Medical College of PLA, vol. 25, no. 6, 2005, pp. 643-6.
Wang SJ, Guo X, Zuo H, et al. [Chondrocyte apoptosis and the expression of Bcl-2, Bax, Fas and iNos in articular cartilage in Kashin-Beck disease]. Di Yi Jun Yi Da Xue Xue Bao. 2005;25(6):643-6.
Wang, S. J., Guo, X., Zuo, H., Zhang, Y. G., Xu, P., Ping, Z. G., Zhang, Z. T., & Geng, D. (2005). [Chondrocyte apoptosis and the expression of Bcl-2, Bax, Fas and iNos in articular cartilage in Kashin-Beck disease]. Di 1 Jun Yi Da Xue Xue Bao = Academic Journal of the First Medical College of PLA, 25(6), 643-6.
Wang SJ, et al. [Chondrocyte Apoptosis and the Expression of Bcl-2, Bax, Fas and iNos in Articular Cartilage in Kashin-Beck Disease]. Di Yi Jun Yi Da Xue Xue Bao. 2005;25(6):643-6. PubMed PMID: 15958298.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Chondrocyte apoptosis and the expression of Bcl-2, Bax, Fas and iNos in articular cartilage in Kashin-Beck disease]. AU - Wang,Shi-jie, AU - Guo,Xiong, AU - Zuo,Hong, AU - Zhang,Yin-gang, AU - Xu,Peng, AU - Ping,Zhi-guang, AU - Zhang,Zeng-tie, AU - Geng,Dong, PY - 2005/6/17/pubmed PY - 2007/6/15/medline PY - 2005/6/17/entrez SP - 643 EP - 6 JF - Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA JO - Di Yi Jun Yi Da Xue Xue Bao VL - 25 IS - 6 N2 - OBJECTIVE: To investigate the characteristics of chondrocyte apoptosis and distribution of Bcl-2, Bax, Fas and iNos expressions in articular cartilage in Kashin-Beck disease (KBD). METHODS: Samples of articular cartilage were collected from 15 healthy children and 15 children with KBD diagnosed according to the Pathological Criteria of KBD Diagnosis in China. Chondrocyte apoptosis was detected by TUNEL method, and the articular chondrocytes positive for Bcl-2, Bax, Fas and iNos were stained by B-SA immunohistochemistry. RESULTS: The percentage of apoptotic chondrocytes positively stained by TUNEL in the middle layer of articular cartilage was significantly higher in KBD children than in the control group (33.60%+/-2.71% vs 1.33%+/-0.41%, t=11.59, P<0.01). Significant difference in Bcl-2, Bax, Fas and iNos expressions was observed between the upper, middle and deep layers of the articular cartilage of KBD children (F =73.49-114.42, P<0.01), and staining for Bcl-2, Bax, Fas and iNos in KBD children was prominent in the upper layer (41.93%+/-12.26%, 45.60%+/-15.78%, 53.60%+/-16.49%, and 45.47%+/-14.02%, respectively) and the middle layer (14.93%+/-3.50%, 13.87%+/-4.32%, 23.27%+/-4.83%, and 21.67%+/-6.82%, respectively) of the articular cartilage; the percentages of chondrocytes positively stained for Bcl-2, Bax, Fas and iNos were significantly higher than those of the control group (t=11.75-18.65, P<0.01). CONCLUSION: The percentages of apoptotic chondrocytes and chondrocytes positive for Bcl-2, Bax, Fas and iNos in the articular cartilage of children with KBD are significantly higher than those in healthy children. SN - 1000-2588 UR - https://www.unboundmedicine.com/medline/citation/15958298/[Chondrocyte_apoptosis_and_the_expression_of_Bcl_2_Bax_Fas_and_iNos_in_articular_cartilage_in_Kashin_Beck_disease]_ L2 - https://medlineplus.gov/osteoarthritis.html DB - PRIME DP - Unbound Medicine ER -