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Inositol 1,4,5-trisphosphate signaling regulates mating behavior in Caenorhabditis elegans males.
Mol Biol Cell. 2005 Sep; 16(9):3978-86.MB

Abstract

Complex behavior requires the coordinated action of the nervous system and nonneuronal targets. Male mating in Caenorhabditis elegans consists of a series of defined behavioral steps that lead to the physiological outcomes required for successful impregnation. We demonstrate that signaling mediated by inositol 1,4,5-trisphosphate (IP(3)) is required at several points during mating. Disruption of IP(3) receptor (itr-1) function results in dramatic loss of male fertility, due to defects in turning behavior (during vulva location), spicule insertion and sperm transfer. To elucidate the signaling pathways responsible, we knocked down the six C. elegans genes encoding phospholipase C (PLC) family members. egl-8, which encodes PLC-beta, functions in spicule insertion and sperm transfer. itr-1 and egl-8 are widely expressed in the male reproductive system. An itr-1 gain-of-function mutation rescues infertility caused by egl-8 RNA interference, indicating that egl-8 and itr-1 function together as central components of the signaling events controlling sperm transfer.

Authors+Show Affiliations

Department of Zoology, University of Cambridge, Cambridge CB2 3EJ, United Kingdom.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15958491

Citation

Gower, Nicholas J D., et al. "Inositol 1,4,5-trisphosphate Signaling Regulates Mating Behavior in Caenorhabditis Elegans Males." Molecular Biology of the Cell, vol. 16, no. 9, 2005, pp. 3978-86.
Gower NJ, Walker DS, Baylis HA. Inositol 1,4,5-trisphosphate signaling regulates mating behavior in Caenorhabditis elegans males. Mol Biol Cell. 2005;16(9):3978-86.
Gower, N. J., Walker, D. S., & Baylis, H. A. (2005). Inositol 1,4,5-trisphosphate signaling regulates mating behavior in Caenorhabditis elegans males. Molecular Biology of the Cell, 16(9), 3978-86.
Gower NJ, Walker DS, Baylis HA. Inositol 1,4,5-trisphosphate Signaling Regulates Mating Behavior in Caenorhabditis Elegans Males. Mol Biol Cell. 2005;16(9):3978-86. PubMed PMID: 15958491.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inositol 1,4,5-trisphosphate signaling regulates mating behavior in Caenorhabditis elegans males. AU - Gower,Nicholas J D, AU - Walker,Denise S, AU - Baylis,Howard A, Y1 - 2005/06/15/ PY - 2005/6/17/pubmed PY - 2006/1/4/medline PY - 2005/6/17/entrez SP - 3978 EP - 86 JF - Molecular biology of the cell JO - Mol Biol Cell VL - 16 IS - 9 N2 - Complex behavior requires the coordinated action of the nervous system and nonneuronal targets. Male mating in Caenorhabditis elegans consists of a series of defined behavioral steps that lead to the physiological outcomes required for successful impregnation. We demonstrate that signaling mediated by inositol 1,4,5-trisphosphate (IP(3)) is required at several points during mating. Disruption of IP(3) receptor (itr-1) function results in dramatic loss of male fertility, due to defects in turning behavior (during vulva location), spicule insertion and sperm transfer. To elucidate the signaling pathways responsible, we knocked down the six C. elegans genes encoding phospholipase C (PLC) family members. egl-8, which encodes PLC-beta, functions in spicule insertion and sperm transfer. itr-1 and egl-8 are widely expressed in the male reproductive system. An itr-1 gain-of-function mutation rescues infertility caused by egl-8 RNA interference, indicating that egl-8 and itr-1 function together as central components of the signaling events controlling sperm transfer. SN - 1059-1524 UR - https://www.unboundmedicine.com/medline/citation/15958491/Inositol_145_trisphosphate_signaling_regulates_mating_behavior_in_Caenorhabditis_elegans_males_ L2 - https://www.molbiolcell.org/doi/10.1091/mbc.e05-02-0096?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -