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Genome sequence of Chrysodeixis chalcites nucleopolyhedrovirus, a baculovirus with two DNA photolyase genes.
J Gen Virol 2005; 86(Pt 7):2069-80JG

Abstract

The complete genome sequence of a single nucleocapsid nucleopolyhedrovirus recently isolated from Chrysodeixis chalcites (ChchNPV) was determined. The viral genome has a size of 149 622 bp and an overall G+C content of 39.1 mol%. The sequence contains 151 predicted open reading frames (ORFs) with a minimal size of 50 codons. The similarity of these ORFs with those of other completely sequenced baculoviruses was calculated using a newly developed database, named GECCO. Phylogenetic analysis of the whole genome confirmed the evolutionary relationship of ChchNPV with group II NPVs, as did the absence of the NPV group I-specific gp64 gene. It is the first group II NPV to encode proliferating cell nuclear antigen. Most noteworthy is the presence of two ORFs encoding a class II cyclobutane pyrimidine dimer DNA photolyase. These two ORFs share only 45 % amino acid identity and have different promoter motifs. Twenty-two additional unique baculovirus genes were identified, including a gene encoding a novel putative RING finger protein with a possible homologue in poxviruses.

Authors+Show Affiliations

Laboratory of Virology, Wageningen University, Binnenhaven 11, 6709 PD Wageningen, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15958686

Citation

van Oers, Monique M., et al. "Genome Sequence of Chrysodeixis Chalcites Nucleopolyhedrovirus, a Baculovirus With Two DNA Photolyase Genes." The Journal of General Virology, vol. 86, no. Pt 7, 2005, pp. 2069-80.
van Oers MM, Abma-Henkens MH, Herniou EA, et al. Genome sequence of Chrysodeixis chalcites nucleopolyhedrovirus, a baculovirus with two DNA photolyase genes. J Gen Virol. 2005;86(Pt 7):2069-80.
van Oers, M. M., Abma-Henkens, M. H., Herniou, E. A., de Groot, J. C., Peters, S., & Vlak, J. M. (2005). Genome sequence of Chrysodeixis chalcites nucleopolyhedrovirus, a baculovirus with two DNA photolyase genes. The Journal of General Virology, 86(Pt 7), pp. 2069-80.
van Oers MM, et al. Genome Sequence of Chrysodeixis Chalcites Nucleopolyhedrovirus, a Baculovirus With Two DNA Photolyase Genes. J Gen Virol. 2005;86(Pt 7):2069-80. PubMed PMID: 15958686.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genome sequence of Chrysodeixis chalcites nucleopolyhedrovirus, a baculovirus with two DNA photolyase genes. AU - van Oers,Monique M, AU - Abma-Henkens,Marleen H C, AU - Herniou,Elisabeth A, AU - de Groot,Joost C W, AU - Peters,Sander, AU - Vlak,Just M, PY - 2005/6/17/pubmed PY - 2005/7/29/medline PY - 2005/6/17/entrez SP - 2069 EP - 80 JF - The Journal of general virology JO - J. Gen. Virol. VL - 86 IS - Pt 7 N2 - The complete genome sequence of a single nucleocapsid nucleopolyhedrovirus recently isolated from Chrysodeixis chalcites (ChchNPV) was determined. The viral genome has a size of 149 622 bp and an overall G+C content of 39.1 mol%. The sequence contains 151 predicted open reading frames (ORFs) with a minimal size of 50 codons. The similarity of these ORFs with those of other completely sequenced baculoviruses was calculated using a newly developed database, named GECCO. Phylogenetic analysis of the whole genome confirmed the evolutionary relationship of ChchNPV with group II NPVs, as did the absence of the NPV group I-specific gp64 gene. It is the first group II NPV to encode proliferating cell nuclear antigen. Most noteworthy is the presence of two ORFs encoding a class II cyclobutane pyrimidine dimer DNA photolyase. These two ORFs share only 45 % amino acid identity and have different promoter motifs. Twenty-two additional unique baculovirus genes were identified, including a gene encoding a novel putative RING finger protein with a possible homologue in poxviruses. SN - 0022-1317 UR - https://www.unboundmedicine.com/medline/citation/15958686/Genome_sequence_of_Chrysodeixis_chalcites_nucleopolyhedrovirus_a_baculovirus_with_two_DNA_photolyase_genes_ L2 - http://jgv.microbiologyresearch.org/pubmed/content/journal/jgv/10.1099/vir.0.80964-0 DB - PRIME DP - Unbound Medicine ER -