Fed-batch fermentation for production of Kluyveromyces marxianus FII 510700 cultivated on a lactose-based medium.
J Ind Microbiol Biotechnol. 2005 Jul; 32(7):284-8.JI

Abstract

A strain of Kluyveromyces marxianus was grown in batch culture in lactose-based media at varying initial lactose concentrations (10-60 g L(-1)) at 30 degrees C, pH 5.0, dissolved oxygen concentrations greater than 20%. Increasing the concentration of mineral salts three-fold at 40 g L(-1) and 60 g L(-1) initial lactose concentration showed only a small increase in the yield of biomass, from 0.38 g g(-1) to 0.41 g g(-1), indicating that the initial batch cultures were not significantly nutrient- (mineral salts)-limited. A relatively high biomass concentration (105 g L(-1)) was obtained in fed-batch culture following extended lactose feeding. An average specific growth rate (0.27 h(-1)), biomass yield (0.38 g g(-1)) and overall productivity (2.9 g L(-1) h(-1)) were obtained for these fed-batch conditions. This fed-batch protocol provides a strategy for achieving relatively high concentrations and productivities of K. marxianus on other lactose-based substrate streams (e.g., whey) from the dairy industry.

Authors+Show Affiliations

Lukondeh T

School of Civil and Environmental Engineering, University of New South Wales, Sydney, Australia. t.lukondeh@unsw.edu.au

Ashbolt NJ

No affiliation info available

Rogers PL

No affiliation info available

MeSH

Culture MediaFermentationKluyveromycesLactoseTime Factors

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15959728

Citation

Lukondeh, Tredwell, et al. "Fed-batch Fermentation for Production of Kluyveromyces Marxianus FII 510700 Cultivated On a Lactose-based Medium." Journal of Industrial Microbiology & Biotechnology, vol. 32, no. 7, 2005, pp. 284-8.

Lukondeh T, Ashbolt NJ, Rogers PL. Fed-batch fermentation for production of Kluyveromyces marxianus FII 510700 cultivated on a lactose-based medium. J Ind Microbiol Biotechnol. 2005;32(7):284-8.

Lukondeh, T., Ashbolt, N. J., & Rogers, P. L. (2005). Fed-batch fermentation for production of Kluyveromyces marxianus FII 510700 cultivated on a lactose-based medium. Journal of Industrial Microbiology & Biotechnology, 32(7), 284-8.

Lukondeh T, Ashbolt NJ, Rogers PL. Fed-batch Fermentation for Production of Kluyveromyces Marxianus FII 510700 Cultivated On a Lactose-based Medium. J Ind Microbiol Biotechnol. 2005;32(7):284-8. PubMed PMID: 15959728.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Fed-batch fermentation for production of Kluyveromyces marxianus FII 510700 cultivated on a lactose-based medium. AU - Lukondeh,Tredwell, AU - Ashbolt,Nicholas J, AU - Rogers,Peter L, Y1 - 2005/06/16/ PY - 2004/05/19/received PY - 2005/05/04/accepted PY - 2005/6/17/pubmed PY - 2005/9/13/medline PY - 2005/6/17/entrez SP - 284 EP - 8 JF - Journal of industrial microbiology & biotechnology JO - J Ind Microbiol Biotechnol VL - 32 IS - 7 N2 - A strain of Kluyveromyces marxianus was grown in batch culture in lactose-based media at varying initial lactose concentrations (10-60 g L(-1)) at 30 degrees C, pH 5.0, dissolved oxygen concentrations greater than 20%. Increasing the concentration of mineral salts three-fold at 40 g L(-1) and 60 g L(-1) initial lactose concentration showed only a small increase in the yield of biomass, from 0.38 g g(-1) to 0.41 g g(-1), indicating that the initial batch cultures were not significantly nutrient- (mineral salts)-limited. A relatively high biomass concentration (105 g L(-1)) was obtained in fed-batch culture following extended lactose feeding. An average specific growth rate (0.27 h(-1)), biomass yield (0.38 g g(-1)) and overall productivity (2.9 g L(-1) h(-1)) were obtained for these fed-batch conditions. This fed-batch protocol provides a strategy for achieving relatively high concentrations and productivities of K. marxianus on other lactose-based substrate streams (e.g., whey) from the dairy industry. SN - 1367-5435 UR - https://www.unboundmedicine.com/medline/citation/15959728/Fed_batch_fermentation_for_production_of_Kluyveromyces_marxianus_FII_510700_cultivated_on_a_lactose_based_medium_ DB - PRIME DP - Unbound Medicine ER -

Tags

Fed-batch fermentation for production of Kluyveromyces marxianus FII 510700 cultivated on a lactose-based medium.J Ind Microbiol Biotechnol. 2005 Jul; 32(7):284-8.JI