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Early expression of KCC2 in rat hippocampal cultures augments expression of functional GABA synapses.
J Physiol 2005; 566(Pt 3):671-9JP

Abstract

The development of GABAergic synapses is associated with an excitatory to inhibitory shift of the actions of GABA because of a reduction of [Cl-]i. This is due to a delayed postnatal expression of the K+ -Cl- cotransporter KCC2, which has low levels at birth and peaks during the first few postnatal weeks. Whether the expression of the cotransporter and the excitatory to inhibitory shift have other consequences on the operation of GABA(A) receptors and synapses is not yet known. We have now expressed KCC2 in immature neurones at an early developmental stage and determined the consequences on the formation of GABA and glutamate synapses. We report that early expression of the cotransporter selectively enhances GABAergic synapses: there is a significant increase of the density of GABA(A) receptors and synapses and an increase of the frequency of GABAergic miniature postsynaptic currents. The density of glutamate synapses and frequency of AMPA miniature postsynaptic currents are not affected. We conclude that the expression of KCC2 and the reduction of [Cl-]i play a critical role in the construction of GABAergic networks that extends beyond the excitatory to inhibitory shift of the actions of GABA.

Authors+Show Affiliations

INMED/INSERM Unite 29, 163 Route de Luminy, 13009 Marseille, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15961425

Citation

Chudotvorova, Ilona, et al. "Early Expression of KCC2 in Rat Hippocampal Cultures Augments Expression of Functional GABA Synapses." The Journal of Physiology, vol. 566, no. Pt 3, 2005, pp. 671-9.
Chudotvorova I, Ivanov A, Rama S, et al. Early expression of KCC2 in rat hippocampal cultures augments expression of functional GABA synapses. J Physiol (Lond). 2005;566(Pt 3):671-9.
Chudotvorova, I., Ivanov, A., Rama, S., Hübner, C. A., Pellegrino, C., Ben-Ari, Y., & Medina, I. (2005). Early expression of KCC2 in rat hippocampal cultures augments expression of functional GABA synapses. The Journal of Physiology, 566(Pt 3), pp. 671-9.
Chudotvorova I, et al. Early Expression of KCC2 in Rat Hippocampal Cultures Augments Expression of Functional GABA Synapses. J Physiol (Lond). 2005 Aug 1;566(Pt 3):671-9. PubMed PMID: 15961425.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Early expression of KCC2 in rat hippocampal cultures augments expression of functional GABA synapses. AU - Chudotvorova,Ilona, AU - Ivanov,Anton, AU - Rama,Sylvain, AU - Hübner,Christian A, AU - Pellegrino,Christophe, AU - Ben-Ari,Yehezkel, AU - Medina,Igor, Y1 - 2005/06/16/ PY - 2005/6/18/pubmed PY - 2005/10/6/medline PY - 2005/6/18/entrez SP - 671 EP - 9 JF - The Journal of physiology JO - J. Physiol. (Lond.) VL - 566 IS - Pt 3 N2 - The development of GABAergic synapses is associated with an excitatory to inhibitory shift of the actions of GABA because of a reduction of [Cl-]i. This is due to a delayed postnatal expression of the K+ -Cl- cotransporter KCC2, which has low levels at birth and peaks during the first few postnatal weeks. Whether the expression of the cotransporter and the excitatory to inhibitory shift have other consequences on the operation of GABA(A) receptors and synapses is not yet known. We have now expressed KCC2 in immature neurones at an early developmental stage and determined the consequences on the formation of GABA and glutamate synapses. We report that early expression of the cotransporter selectively enhances GABAergic synapses: there is a significant increase of the density of GABA(A) receptors and synapses and an increase of the frequency of GABAergic miniature postsynaptic currents. The density of glutamate synapses and frequency of AMPA miniature postsynaptic currents are not affected. We conclude that the expression of KCC2 and the reduction of [Cl-]i play a critical role in the construction of GABAergic networks that extends beyond the excitatory to inhibitory shift of the actions of GABA. SN - 0022-3751 UR - https://www.unboundmedicine.com/medline/citation/15961425/Early_expression_of_KCC2_in_rat_hippocampal_cultures_augments_expression_of_functional_GABA_synapses_ L2 - https://doi.org/10.1113/jphysiol.2005.089821 DB - PRIME DP - Unbound Medicine ER -