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Polarization of naive T cells into Th1 or Th2 by distinct cytokine-driven murine dendritic cell populations: implications for immunotherapy.
J Leukoc Biol. 2005 Sep; 78(3):656-64.JL

Abstract

Dendritic cells (DCs) activate T cells and regulate their differentiation into T helper cell type 1 (Th1) and/or Th2 cells. To identify DCs with differing abilities to direct Th1/Th2 cell differentiation, we cultured mouse bone marrow progenitors in granulocyte macrophage-colony stimulating factor (GM), GM + interleukin (IL)-4, or GM + IL-15 and generated three distinct DC populations. The GM + IL-4 DCs expressed high levels of CD80/CD86 and major histocompatibility complex (MHC) class II and produced low levels of IL-12p70. GM and GM + IL-15 DCs expressed low levels of CD80/CD86 and MHC class II. The GM + IL-15 DCs produced high levels of IL-12p70 and interferon (IFN)-gamma, whereas GM DCs produced only high levels of IL-12p70. Naive T cells stimulated with GM + IL-4 DCs secreted high levels of IL-4 and IL-5 in addition to IFN-gamma. In contrast, the GM + IL-15 DCs induced higher IFN-gamma production by T cells with little or no Th2 cytokines. GM DCs did not induce T cell polarization, despite producing large amounts of IL-12p70 following activation. A similar pattern of T cell activation was observed after in vivo administration of DCs. These data suggest that IL-12p70 production alone, although necessary for Th1 differentiation, is not sufficient to induce Th1 responses. These studies have implications for the use of DC-based vaccines in immunotherapy of cancer and other clinical conditions.

Authors+Show Affiliations

Division of Plastic Surgery, University of Pittsburgh, Scaife Hall, Suite 666, 3550 Terrace St., Pittsburgh, PA 15261, USA. haririmf@upmc.eduNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15961574

Citation

Feili-Hariri, Maryam, et al. "Polarization of Naive T Cells Into Th1 or Th2 By Distinct Cytokine-driven Murine Dendritic Cell Populations: Implications for Immunotherapy." Journal of Leukocyte Biology, vol. 78, no. 3, 2005, pp. 656-64.
Feili-Hariri M, Falkner DH, Morel PA. Polarization of naive T cells into Th1 or Th2 by distinct cytokine-driven murine dendritic cell populations: implications for immunotherapy. J Leukoc Biol. 2005;78(3):656-64.
Feili-Hariri, M., Falkner, D. H., & Morel, P. A. (2005). Polarization of naive T cells into Th1 or Th2 by distinct cytokine-driven murine dendritic cell populations: implications for immunotherapy. Journal of Leukocyte Biology, 78(3), 656-64.
Feili-Hariri M, Falkner DH, Morel PA. Polarization of Naive T Cells Into Th1 or Th2 By Distinct Cytokine-driven Murine Dendritic Cell Populations: Implications for Immunotherapy. J Leukoc Biol. 2005;78(3):656-64. PubMed PMID: 15961574.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polarization of naive T cells into Th1 or Th2 by distinct cytokine-driven murine dendritic cell populations: implications for immunotherapy. AU - Feili-Hariri,Maryam, AU - Falkner,Dewayne H, AU - Morel,Penelope A, Y1 - 2005/06/16/ PY - 2005/6/18/pubmed PY - 2006/1/13/medline PY - 2005/6/18/entrez SP - 656 EP - 64 JF - Journal of leukocyte biology JO - J Leukoc Biol VL - 78 IS - 3 N2 - Dendritic cells (DCs) activate T cells and regulate their differentiation into T helper cell type 1 (Th1) and/or Th2 cells. To identify DCs with differing abilities to direct Th1/Th2 cell differentiation, we cultured mouse bone marrow progenitors in granulocyte macrophage-colony stimulating factor (GM), GM + interleukin (IL)-4, or GM + IL-15 and generated three distinct DC populations. The GM + IL-4 DCs expressed high levels of CD80/CD86 and major histocompatibility complex (MHC) class II and produced low levels of IL-12p70. GM and GM + IL-15 DCs expressed low levels of CD80/CD86 and MHC class II. The GM + IL-15 DCs produced high levels of IL-12p70 and interferon (IFN)-gamma, whereas GM DCs produced only high levels of IL-12p70. Naive T cells stimulated with GM + IL-4 DCs secreted high levels of IL-4 and IL-5 in addition to IFN-gamma. In contrast, the GM + IL-15 DCs induced higher IFN-gamma production by T cells with little or no Th2 cytokines. GM DCs did not induce T cell polarization, despite producing large amounts of IL-12p70 following activation. A similar pattern of T cell activation was observed after in vivo administration of DCs. These data suggest that IL-12p70 production alone, although necessary for Th1 differentiation, is not sufficient to induce Th1 responses. These studies have implications for the use of DC-based vaccines in immunotherapy of cancer and other clinical conditions. SN - 0741-5400 UR - https://www.unboundmedicine.com/medline/citation/15961574/Polarization_of_naive_T_cells_into_Th1_or_Th2_by_distinct_cytokine_driven_murine_dendritic_cell_populations:_implications_for_immunotherapy_ L2 - https://doi.org/10.1189/jlb.1104631 DB - PRIME DP - Unbound Medicine ER -