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Inhibitory effect of Buthus martensi Karsch extracts on interleukin-1beta-induced expression of nitric oxide (NO) synthase and production of NO in human chondrocytes and LPS-induced NO and prostaglandin E2 production in mouse peritoneal macrophages.
Toxicol In Vitro. 2005 Sep; 19(6):757-69.TV

Abstract

We examined the effect of Buthus martensi Karsch (BMK) extract on IL-1beta-induced production of nitrogen oxide (NO) in primary human osteoarthritis (OA) chondrocytes. The cells were treated with BMK (10 microg/ml) and IL-1beta (2 ng/ml) for different periods, and inducible nitric oxide synthase (iNOS) mRNA and protein expression were determined by real-time quantitative reverse transcriptase-polymerase chain reaction and Western blotting, respectively. The cytotoxicity of BMK on human OA chondrocytes was very low (IC50 > 250 microg/ml) as measured by the XTT assay method. Production of NO was determined as nitrite in culture supernatant. Human chondrocytes cotreated with BMK produced significantly less NO compared with chondrocytes stimulated with IL-1beta alone. Activation and translocation of and NF-(kappa)B DNA binding activity were determined by Western blotting and specific enzyme-linked immunosorbent assay. The inhibition of NO production correlated with the suppression of induction and expression of nuclear factor-kappaB (NF-(kappa)B) and activation protein-1 (AP-1)-dependent gene. BMK inhibited the activation and translocation of NF-(kappa)B to the nucleus, indicating that BMK inhibits the IL-1beta-induced production of NO in human chondrocytes by interfering with the activation of NF-(kappa)B through a novel mechanism. In addition, BMK reduced prostaglandin E2 (PGE2) production in mouse peritoneal macrophages stimulated with lipopolysaccharide, whereas no influence on the activity of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2) or cyclooxygenase-1 (COX-1) was observed. Our data, therefore, suggest that BMK may be a therapeutically effective inhibitor of IL-1beta-induced inflammatory effects that are dependent on NF-(kappa)B activation in human OA chondrocytes. The results indicate that BMK exerts anti-inflammatory effects related to the inhibition of neutrophil functions and of NO and PGE2 production, which could be due to a decreased expression of iNOS and COX-2 through the transcription factors NF-(kappa)B and AP-1.

Authors+Show Affiliations

Department of Biochemistry, Molecular Biology and Acupuncture, College of Oriental Medicine, Dongguk University, Kyungju, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15963682

Citation

Kim, Kap-Sung, et al. "Inhibitory Effect of Buthus Martensi Karsch Extracts On Interleukin-1beta-induced Expression of Nitric Oxide (NO) Synthase and Production of NO in Human Chondrocytes and LPS-induced NO and Prostaglandin E2 Production in Mouse Peritoneal Macrophages." Toxicology in Vitro : an International Journal Published in Association With BIBRA, vol. 19, no. 6, 2005, pp. 757-69.
Kim KS, Cho HS, Lee SD, et al. Inhibitory effect of Buthus martensi Karsch extracts on interleukin-1beta-induced expression of nitric oxide (NO) synthase and production of NO in human chondrocytes and LPS-induced NO and prostaglandin E2 production in mouse peritoneal macrophages. Toxicol In Vitro. 2005;19(6):757-69.
Kim, K. S., Cho, H. S., Lee, S. D., Kim, K. H., Cho, J. Y., Chung, K. H., Lee, Y. C., Moon, S. K., & Kim, C. H. (2005). Inhibitory effect of Buthus martensi Karsch extracts on interleukin-1beta-induced expression of nitric oxide (NO) synthase and production of NO in human chondrocytes and LPS-induced NO and prostaglandin E2 production in mouse peritoneal macrophages. Toxicology in Vitro : an International Journal Published in Association With BIBRA, 19(6), 757-69.
Kim KS, et al. Inhibitory Effect of Buthus Martensi Karsch Extracts On Interleukin-1beta-induced Expression of Nitric Oxide (NO) Synthase and Production of NO in Human Chondrocytes and LPS-induced NO and Prostaglandin E2 Production in Mouse Peritoneal Macrophages. Toxicol In Vitro. 2005;19(6):757-69. PubMed PMID: 15963682.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibitory effect of Buthus martensi Karsch extracts on interleukin-1beta-induced expression of nitric oxide (NO) synthase and production of NO in human chondrocytes and LPS-induced NO and prostaglandin E2 production in mouse peritoneal macrophages. AU - Kim,Kap-Sung, AU - Cho,Hyun-Seok, AU - Lee,Seung-Deok, AU - Kim,Kyung-Ho, AU - Cho,Jae-Yong, AU - Chung,Kang-Hyun, AU - Lee,Young-Choon, AU - Moon,Sung-Kwon, AU - Kim,Cheorl-Ho, PY - 2004/10/11/received PY - 2005/03/13/revised PY - 2005/04/25/accepted PY - 2005/6/21/pubmed PY - 2005/9/28/medline PY - 2005/6/21/entrez SP - 757 EP - 69 JF - Toxicology in vitro : an international journal published in association with BIBRA JO - Toxicol In Vitro VL - 19 IS - 6 N2 - We examined the effect of Buthus martensi Karsch (BMK) extract on IL-1beta-induced production of nitrogen oxide (NO) in primary human osteoarthritis (OA) chondrocytes. The cells were treated with BMK (10 microg/ml) and IL-1beta (2 ng/ml) for different periods, and inducible nitric oxide synthase (iNOS) mRNA and protein expression were determined by real-time quantitative reverse transcriptase-polymerase chain reaction and Western blotting, respectively. The cytotoxicity of BMK on human OA chondrocytes was very low (IC50 > 250 microg/ml) as measured by the XTT assay method. Production of NO was determined as nitrite in culture supernatant. Human chondrocytes cotreated with BMK produced significantly less NO compared with chondrocytes stimulated with IL-1beta alone. Activation and translocation of and NF-(kappa)B DNA binding activity were determined by Western blotting and specific enzyme-linked immunosorbent assay. The inhibition of NO production correlated with the suppression of induction and expression of nuclear factor-kappaB (NF-(kappa)B) and activation protein-1 (AP-1)-dependent gene. BMK inhibited the activation and translocation of NF-(kappa)B to the nucleus, indicating that BMK inhibits the IL-1beta-induced production of NO in human chondrocytes by interfering with the activation of NF-(kappa)B through a novel mechanism. In addition, BMK reduced prostaglandin E2 (PGE2) production in mouse peritoneal macrophages stimulated with lipopolysaccharide, whereas no influence on the activity of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2) or cyclooxygenase-1 (COX-1) was observed. Our data, therefore, suggest that BMK may be a therapeutically effective inhibitor of IL-1beta-induced inflammatory effects that are dependent on NF-(kappa)B activation in human OA chondrocytes. The results indicate that BMK exerts anti-inflammatory effects related to the inhibition of neutrophil functions and of NO and PGE2 production, which could be due to a decreased expression of iNOS and COX-2 through the transcription factors NF-(kappa)B and AP-1. SN - 0887-2333 UR - https://www.unboundmedicine.com/medline/citation/15963682/Inhibitory_effect_of_Buthus_martensi_Karsch_extracts_on_interleukin_1beta_induced_expression_of_nitric_oxide__NO__synthase_and_production_of_NO_in_human_chondrocytes_and_LPS_induced_NO_and_prostaglandin_E2_production_in_mouse_peritoneal_macrophages_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0887-2333(05)00069-X DB - PRIME DP - Unbound Medicine ER -