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Depressor responses to L-proline microinjected into the rat ventrolateral medulla are mediated by ionotropic excitatory amino acid receptors.
Auton Neurosci. 2005 Jun 15; 120(1-2):108-12.AN

Abstract

The essential amino acid L-proline produces a depressor response when microinjected into the caudal ventrolateral medulla (CVLM) of anesthetized rats. L-proline may activate some excitatory amino acid (EAA) receptors. The present study tested this hypothesis by investigating the effects of two ionotropic excitatory amino acid receptor antagonists on the depressor response to L-proline in the CVLM: the alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA)/kainate receptor-selective antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) and the N-methyl-D-aspartate (NMDA) receptor-selective antagonist MK801. Urethane-anesthetized rats received arterial catheters and their ventrolateral medulla surface was exposed. Injections of the antagonists CNQX and MK801 (2 mM, 68 nl in each case) into the CVLM completely blocked depressor responses to subsequent administration of AMPA (2 pmol/34 nl) and NMDA (2 pmol/34 nl), respectively. The depressor response to L-proline (3.4 nmol/34 nl) was strongly inhibited by prior injection of CNQX (2 mM, 68 nl) and significantly attenuated by prior injection of a high dose (20 mM, 68 nl), but not a low dose (2 mM, 68 nl), of MK801. The results indicate that the depressor response to L-proline in the CVLM includes mechanisms of ionotropic excitatory amino acid receptors.

Authors+Show Affiliations

Department of Neurophysiology, Division of Integrated Medical Science, Programs for Biomedical Research, Graduate School of Biomedical Sciences, Hiroshima University, Japan. yumitake@hiroshima-u.ac.jp

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15964784

Citation

Takemoto, Yumi. "Depressor Responses to L-proline Microinjected Into the Rat Ventrolateral Medulla Are Mediated By Ionotropic Excitatory Amino Acid Receptors." Autonomic Neuroscience : Basic & Clinical, vol. 120, no. 1-2, 2005, pp. 108-12.
Takemoto Y. Depressor responses to L-proline microinjected into the rat ventrolateral medulla are mediated by ionotropic excitatory amino acid receptors. Auton Neurosci. 2005;120(1-2):108-12.
Takemoto, Y. (2005). Depressor responses to L-proline microinjected into the rat ventrolateral medulla are mediated by ionotropic excitatory amino acid receptors. Autonomic Neuroscience : Basic & Clinical, 120(1-2), 108-12.
Takemoto Y. Depressor Responses to L-proline Microinjected Into the Rat Ventrolateral Medulla Are Mediated By Ionotropic Excitatory Amino Acid Receptors. Auton Neurosci. 2005 Jun 15;120(1-2):108-12. PubMed PMID: 15964784.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Depressor responses to L-proline microinjected into the rat ventrolateral medulla are mediated by ionotropic excitatory amino acid receptors. A1 - Takemoto,Yumi, PY - 2005/01/31/received PY - 2005/05/06/revised PY - 2005/05/10/accepted PY - 2005/6/21/pubmed PY - 2005/9/1/medline PY - 2005/6/21/entrez SP - 108 EP - 12 JF - Autonomic neuroscience : basic & clinical JO - Auton Neurosci VL - 120 IS - 1-2 N2 - The essential amino acid L-proline produces a depressor response when microinjected into the caudal ventrolateral medulla (CVLM) of anesthetized rats. L-proline may activate some excitatory amino acid (EAA) receptors. The present study tested this hypothesis by investigating the effects of two ionotropic excitatory amino acid receptor antagonists on the depressor response to L-proline in the CVLM: the alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA)/kainate receptor-selective antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) and the N-methyl-D-aspartate (NMDA) receptor-selective antagonist MK801. Urethane-anesthetized rats received arterial catheters and their ventrolateral medulla surface was exposed. Injections of the antagonists CNQX and MK801 (2 mM, 68 nl in each case) into the CVLM completely blocked depressor responses to subsequent administration of AMPA (2 pmol/34 nl) and NMDA (2 pmol/34 nl), respectively. The depressor response to L-proline (3.4 nmol/34 nl) was strongly inhibited by prior injection of CNQX (2 mM, 68 nl) and significantly attenuated by prior injection of a high dose (20 mM, 68 nl), but not a low dose (2 mM, 68 nl), of MK801. The results indicate that the depressor response to L-proline in the CVLM includes mechanisms of ionotropic excitatory amino acid receptors. SN - 1566-0702 UR - https://www.unboundmedicine.com/medline/citation/15964784/Depressor_responses_to_L_proline_microinjected_into_the_rat_ventrolateral_medulla_are_mediated_by_ionotropic_excitatory_amino_acid_receptors_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1566-0702(05)00100-1 DB - PRIME DP - Unbound Medicine ER -