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Detection and quantitation of a bioactive compound in Vicia narbonensis L. seeds by capillary electrophoresis-mass spectrometry: a comparative study with UV detection.
Electrophoresis. 2005 Jun; 26(12):2351-9.E

Abstract

Capillary zone electrophoresis with mass spectrometry (CE-MS) and UV detection (CE-UV) was applied to the quantitative determination of gamma-glutamyl-S-ethenyl-cysteine (GEC), a bioactive and unstable compound present in Vicia narbonensis L. seeds. This compound is responsible for, among other negative effects, palatability reduction and grain toxicity. In order to carry out the quantitative analysis of GEC, different conditions (such as composition, concentration and pH of the background electrolyte, and type and time of extraction) were studied. Also, adequate conditions for electrospray-mass spectrometry of this bioactive compound were investigated. The best extraction conditions of GEC from V. narbonensis L. seeds flour were obtained using ethanol-water (70:30 v/v) for 45 min. The use of a 20 m ammonium hydrogen carbonate at pH 7 provided adequate analytical conditions compatible with the unstable nature of GEC as well as with the requirements of CE-UV and CE-MS analysis. A comparative study was carried out between the different figures of merit of CE-UV and CE-MS for quantitative purposes. Both techniques provided similar limit of detection and can be applied with confidence within the same linear dynamic range. However, reproducibility and speed of analysis were better using CE-UV. The developed methods were readily applied to quantify GEC in seeds of 21 genotypes of V. narbonensis L. A good agreement between CE-MS and CE-UV results was observed corroborating the usefulness of both approaches for quantitative purposes.

Authors+Show Affiliations

Arias M
CIA de Albaladejito, Cuenca, Spain.
Simó C
No affiliation info available
Ortiz LT
No affiliation info available
de Ios Mozos-Pascual M
No affiliation info available
Barbas C
No affiliation info available
Cifuentes A
No affiliation info available

MeSH

DipeptidesElectrophoresis, CapillaryMass SpectrometrySeedsSensitivity and SpecificitySpectrometry, Mass, Electrospray IonizationSpectrophotometry, UltravioletTasteVicia

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15966020

Citation

Arias, Marina, et al. "Detection and Quantitation of a Bioactive Compound in Vicia Narbonensis L. Seeds By Capillary Electrophoresis-mass Spectrometry: a Comparative Study With UV Detection." Electrophoresis, vol. 26, no. 12, 2005, pp. 2351-9.
Arias M, Simó C, Ortiz LT, et al. Detection and quantitation of a bioactive compound in Vicia narbonensis L. seeds by capillary electrophoresis-mass spectrometry: a comparative study with UV detection. Electrophoresis. 2005;26(12):2351-9.
Arias, M., Simó, C., Ortiz, L. T., de Ios Mozos-Pascual, M., Barbas, C., & Cifuentes, A. (2005). Detection and quantitation of a bioactive compound in Vicia narbonensis L. seeds by capillary electrophoresis-mass spectrometry: a comparative study with UV detection. Electrophoresis, 26(12), 2351-9.
Arias M, et al. Detection and Quantitation of a Bioactive Compound in Vicia Narbonensis L. Seeds By Capillary Electrophoresis-mass Spectrometry: a Comparative Study With UV Detection. Electrophoresis. 2005;26(12):2351-9. PubMed PMID: 15966020.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection and quantitation of a bioactive compound in Vicia narbonensis L. seeds by capillary electrophoresis-mass spectrometry: a comparative study with UV detection. AU - Arias,Marina, AU - Simó,Carolina, AU - Ortiz,Luis T, AU - de Ios Mozos-Pascual,Marcelino, AU - Barbas,Coral, AU - Cifuentes,Alejandro, PY - 2005/6/21/pubmed PY - 2005/9/20/medline PY - 2005/6/21/entrez SP - 2351 EP - 9 JF - Electrophoresis JO - Electrophoresis VL - 26 IS - 12 N2 - Capillary zone electrophoresis with mass spectrometry (CE-MS) and UV detection (CE-UV) was applied to the quantitative determination of gamma-glutamyl-S-ethenyl-cysteine (GEC), a bioactive and unstable compound present in Vicia narbonensis L. seeds. This compound is responsible for, among other negative effects, palatability reduction and grain toxicity. In order to carry out the quantitative analysis of GEC, different conditions (such as composition, concentration and pH of the background electrolyte, and type and time of extraction) were studied. Also, adequate conditions for electrospray-mass spectrometry of this bioactive compound were investigated. The best extraction conditions of GEC from V. narbonensis L. seeds flour were obtained using ethanol-water (70:30 v/v) for 45 min. The use of a 20 m ammonium hydrogen carbonate at pH 7 provided adequate analytical conditions compatible with the unstable nature of GEC as well as with the requirements of CE-UV and CE-MS analysis. A comparative study was carried out between the different figures of merit of CE-UV and CE-MS for quantitative purposes. Both techniques provided similar limit of detection and can be applied with confidence within the same linear dynamic range. However, reproducibility and speed of analysis were better using CE-UV. The developed methods were readily applied to quantify GEC in seeds of 21 genotypes of V. narbonensis L. A good agreement between CE-MS and CE-UV results was observed corroborating the usefulness of both approaches for quantitative purposes. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/15966020/Detection_and_quantitation_of_a_bioactive_compound_in_Vicia_narbonensis_L__seeds_by_capillary_electrophoresis_mass_spectrometry:_a_comparative_study_with_UV_detection_ DB - PRIME DP - Unbound Medicine ER -