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[Over-expression in Escherichia coli and purification of nucleocaspid and membrane protein of SARS coronavirus].
Sheng Wu Gong Cheng Xue Bao. 2003 Jul; 19(4):392-6.SW

Abstract

Genes encoding nucleocaspid (N) and membrane (M) protein of SARS coronavirus were obtained by RT-PCR and were cloned into expression vector pET22b and pBV222. DNA sequencing showed that the genes cloned from a patient in Beijing were identical to the gene sequences from reported Toronto strain. The genes were over-expressed in E. coli either as inclusion body or as soluble form. The recombinant proteins were purified by ion-exchange, or ion-exchange followed by metal chelate affinity chromatography. The recombinant N protein was demonstrated highly antigenic and could be employed as antigen to detect SARS antibodies in ELISA system for SARS diagnosis.

Authors+Show Affiliations

Yi YP
Beijing Institute of Biotechnology, Beijing 100850, China.
Li CF
No affiliation info available
Shi YL
No affiliation info available
Li LH
No affiliation info available
Li P
No affiliation info available
Huang W
No affiliation info available
Wang SQ
No affiliation info available
Ma QJ
No affiliation info available
Cao C
No affiliation info available

MeSH

Chromatography, AffinityChromatography, Ion ExchangeEnzyme-Linked Immunosorbent AssayEscherichia coliNucleocapsid ProteinsReverse Transcriptase Polymerase Chain ReactionSARS VirusViral Structural Proteins

Pub Type(s)

Journal Article

Language

chi

PubMed ID

15969052

Citation

Yi, Yan-Ping, et al. "[Over-expression in Escherichia Coli and Purification of Nucleocaspid and Membrane Protein of SARS Coronavirus]." Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology, vol. 19, no. 4, 2003, pp. 392-6.
Yi YP, Li CF, Shi YL, et al. [Over-expression in Escherichia coli and purification of nucleocaspid and membrane protein of SARS coronavirus]. Sheng Wu Gong Cheng Xue Bao. 2003;19(4):392-6.
Yi, Y. P., Li, C. F., Shi, Y. L., Li, L. H., Li, P., Huang, W., Wang, S. Q., Ma, Q. J., & Cao, C. (2003). [Over-expression in Escherichia coli and purification of nucleocaspid and membrane protein of SARS coronavirus]. Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology, 19(4), 392-6.
Yi YP, et al. [Over-expression in Escherichia Coli and Purification of Nucleocaspid and Membrane Protein of SARS Coronavirus]. Sheng Wu Gong Cheng Xue Bao. 2003;19(4):392-6. PubMed PMID: 15969052.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Over-expression in Escherichia coli and purification of nucleocaspid and membrane protein of SARS coronavirus]. AU - Yi,Yan-Ping, AU - Li,Chu-Fang, AU - Shi,Yu-Ling, AU - Li,Lin-Hai, AU - Li,Ping, AU - Huang,Wei, AU - Wang,Sheng-Qi, AU - Ma,Qing-Jun, AU - Cao,Cheng, PY - 2005/6/23/pubmed PY - 2010/4/30/medline PY - 2005/6/23/entrez SP - 392 EP - 6 JF - Sheng wu gong cheng xue bao = Chinese journal of biotechnology JO - Sheng Wu Gong Cheng Xue Bao VL - 19 IS - 4 N2 - Genes encoding nucleocaspid (N) and membrane (M) protein of SARS coronavirus were obtained by RT-PCR and were cloned into expression vector pET22b and pBV222. DNA sequencing showed that the genes cloned from a patient in Beijing were identical to the gene sequences from reported Toronto strain. The genes were over-expressed in E. coli either as inclusion body or as soluble form. The recombinant proteins were purified by ion-exchange, or ion-exchange followed by metal chelate affinity chromatography. The recombinant N protein was demonstrated highly antigenic and could be employed as antigen to detect SARS antibodies in ELISA system for SARS diagnosis. SN - 1000-3061 UR - https://www.unboundmedicine.com/medline/citation/15969052/[Over_expression_in_Escherichia_coli_and_purification_of_nucleocaspid_and_membrane_protein_of_SARS_coronavirus]_ DB - PRIME DP - Unbound Medicine ER -