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[Over-expression in Escherichia coli and purification of nucleocaspid and membrane protein of SARS coronavirus].
Sheng Wu Gong Cheng Xue Bao. 2003 Jul; 19(4):392-6.SW

Abstract

Genes encoding nucleocaspid (N) and membrane (M) protein of SARS coronavirus were obtained by RT-PCR and were cloned into expression vector pET22b and pBV222. DNA sequencing showed that the genes cloned from a patient in Beijing were identical to the gene sequences from reported Toronto strain. The genes were over-expressed in E. coli either as inclusion body or as soluble form. The recombinant proteins were purified by ion-exchange, or ion-exchange followed by metal chelate affinity chromatography. The recombinant N protein was demonstrated highly antigenic and could be employed as antigen to detect SARS antibodies in ELISA system for SARS diagnosis.

Authors+Show Affiliations

Beijing Institute of Biotechnology, Beijing 100850, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

chi

PubMed ID

15969052

Citation

Yi, Yan-Ping, et al. "[Over-expression in Escherichia Coli and Purification of Nucleocaspid and Membrane Protein of SARS Coronavirus]." Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology, vol. 19, no. 4, 2003, pp. 392-6.
Yi YP, Li CF, Shi YL, et al. [Over-expression in Escherichia coli and purification of nucleocaspid and membrane protein of SARS coronavirus]. Sheng Wu Gong Cheng Xue Bao. 2003;19(4):392-6.
Yi, Y. P., Li, C. F., Shi, Y. L., Li, L. H., Li, P., Huang, W., Wang, S. Q., Ma, Q. J., & Cao, C. (2003). [Over-expression in Escherichia coli and purification of nucleocaspid and membrane protein of SARS coronavirus]. Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology, 19(4), 392-6.
Yi YP, et al. [Over-expression in Escherichia Coli and Purification of Nucleocaspid and Membrane Protein of SARS Coronavirus]. Sheng Wu Gong Cheng Xue Bao. 2003;19(4):392-6. PubMed PMID: 15969052.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Over-expression in Escherichia coli and purification of nucleocaspid and membrane protein of SARS coronavirus]. AU - Yi,Yan-Ping, AU - Li,Chu-Fang, AU - Shi,Yu-Ling, AU - Li,Lin-Hai, AU - Li,Ping, AU - Huang,Wei, AU - Wang,Sheng-Qi, AU - Ma,Qing-Jun, AU - Cao,Cheng, PY - 2005/6/23/pubmed PY - 2010/4/30/medline PY - 2005/6/23/entrez SP - 392 EP - 6 JF - Sheng wu gong cheng xue bao = Chinese journal of biotechnology JO - Sheng Wu Gong Cheng Xue Bao VL - 19 IS - 4 N2 - Genes encoding nucleocaspid (N) and membrane (M) protein of SARS coronavirus were obtained by RT-PCR and were cloned into expression vector pET22b and pBV222. DNA sequencing showed that the genes cloned from a patient in Beijing were identical to the gene sequences from reported Toronto strain. The genes were over-expressed in E. coli either as inclusion body or as soluble form. The recombinant proteins were purified by ion-exchange, or ion-exchange followed by metal chelate affinity chromatography. The recombinant N protein was demonstrated highly antigenic and could be employed as antigen to detect SARS antibodies in ELISA system for SARS diagnosis. SN - 1000-3061 UR - https://www.unboundmedicine.com/medline/citation/15969052/[Over_expression_in_Escherichia_coli_and_purification_of_nucleocaspid_and_membrane_protein_of_SARS_coronavirus]_ DB - PRIME DP - Unbound Medicine ER -