Tags

Type your tag names separated by a space and hit enter

Verification of clinical samples, positive in AMPLICOR Neisseria gonorrhoeae polymerase chain reaction, by 16S rRNA and gyrA compared with culture.
Int J STD AIDS. 2005 Jun; 16(6):415-9.IJ

Abstract

We compared 956 samples for AMPLICOR Neisseria gonorrhoeae polymerase chain reaction (PCR) (Roche) with species verification using the 16S rRNA gene to verification using gyrA gene. Control was the culture method. The gyrA verification uses pyrosequencing of the quinolone resistance-determining region of gyrA. Of 52 samples with optical density >/=0.2 in PCR, 27 were negative in culture, two samples from pharynx were false negative in culture and four samples from pharynx were false positives in verification with 16S rRNA. Twenty-five samples showed growth of gonococci, 18 of the corresponding PCR samples were verified by both methods; three urine samples were positive only in gyrA ; and one pharynx specimen was positive only in 16S rRNA. Three samples were lost. We conclude that AMPLICOR N. gonorrhoeae PCR with verification in gyrA gene can be considered as a diagnostic tool in populations with low prevalence of gonorrhoea and that pharynx specimens should not be analysed by PCR.

Authors+Show Affiliations

Department of Laboratory Medicine, Division of Clinical Bacteriology, F82 Karolinska University Hospital Huddinge, SE-141 86 Stockholm, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

15969775

Citation

Airell, Asa, et al. "Verification of Clinical Samples, Positive in AMPLICOR Neisseria Gonorrhoeae Polymerase Chain Reaction, By 16S rRNA and gyrA Compared With Culture." International Journal of STD & AIDS, vol. 16, no. 6, 2005, pp. 415-9.
Airell A, Lindbäck E, Ataker F, et al. Verification of clinical samples, positive in AMPLICOR Neisseria gonorrhoeae polymerase chain reaction, by 16S rRNA and gyrA compared with culture. Int J STD AIDS. 2005;16(6):415-9.
Airell, A., Lindbäck, E., Ataker, F., Pörnull, K. J., & Wretlind, B. (2005). Verification of clinical samples, positive in AMPLICOR Neisseria gonorrhoeae polymerase chain reaction, by 16S rRNA and gyrA compared with culture. International Journal of STD & AIDS, 16(6), 415-9.
Airell A, et al. Verification of Clinical Samples, Positive in AMPLICOR Neisseria Gonorrhoeae Polymerase Chain Reaction, By 16S rRNA and gyrA Compared With Culture. Int J STD AIDS. 2005;16(6):415-9. PubMed PMID: 15969775.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Verification of clinical samples, positive in AMPLICOR Neisseria gonorrhoeae polymerase chain reaction, by 16S rRNA and gyrA compared with culture. AU - Airell,Asa, AU - Lindbäck,Emma, AU - Ataker,Ferda, AU - Pörnull,Kirsti Jalakas, AU - Wretlind,Bengt, PY - 2005/6/23/pubmed PY - 2005/8/23/medline PY - 2005/6/23/entrez SP - 415 EP - 9 JF - International journal of STD & AIDS JO - Int J STD AIDS VL - 16 IS - 6 N2 - We compared 956 samples for AMPLICOR Neisseria gonorrhoeae polymerase chain reaction (PCR) (Roche) with species verification using the 16S rRNA gene to verification using gyrA gene. Control was the culture method. The gyrA verification uses pyrosequencing of the quinolone resistance-determining region of gyrA. Of 52 samples with optical density >/=0.2 in PCR, 27 were negative in culture, two samples from pharynx were false negative in culture and four samples from pharynx were false positives in verification with 16S rRNA. Twenty-five samples showed growth of gonococci, 18 of the corresponding PCR samples were verified by both methods; three urine samples were positive only in gyrA ; and one pharynx specimen was positive only in 16S rRNA. Three samples were lost. We conclude that AMPLICOR N. gonorrhoeae PCR with verification in gyrA gene can be considered as a diagnostic tool in populations with low prevalence of gonorrhoea and that pharynx specimens should not be analysed by PCR. SN - 0956-4624 UR - https://www.unboundmedicine.com/medline/citation/15969775/Verification_of_clinical_samples_positive_in_AMPLICOR_Neisseria_gonorrhoeae_polymerase_chain_reaction_by_16S_rRNA_and_gyrA_compared_with_culture_ L2 - http://journals.sagepub.com/doi/full/10.1258/0956462054094024?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -