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Antioxidant activity of Crataegus aronia aqueous extract used in traditional Arab medicine in Israel.
J Ethnopharmacol. 2005 Oct 03; 101(1-3):153-61.JE

Abstract

BACKGROUND

The medicinal use of extracts prepared from plant parts of the genus Crataegus dates back to ancient times. Furthermore, it has been proposed that its antioxidant constituents account for its beneficial therapeutic effects. A decoction of leaves and unripe fruits from Crataegus aronia syn. azarolus (L) (Rosaceae), the indigenous Israeli hawthorn, is used to treat cardiovascular diseases, cancer, diabetes and sexual weakness in Arab traditional medicine.

PURPOSE

Because laboratory data on the bioactivity of extracts prepared from the indigenous Israeli hawthorn is lacking, we evaluated the antioxidant and cytotoxic potentials of an extract prepared from leaves and unripe fruits in a variety of cell and cell-free in vitro assays.

METHODS

The antioxidant assays measured: (a) its ability to inhibit (i) oxidation of beta-carotene, (ii) 2,2'-azobis(2-amidino-propan) dihydrochloride (AAPH)-induced plasma oxidation and (iii) iron-induced lipid peroxidation in rat liver homogenates; (b) its ability to scavenge the superoxide (O2-) radical; (c) its effects on the enzyme xanthine oxidase (XO) activity; (d) its effect on the redox state of glutathione (GSH) in cultured Hep G2 cells. In addition, we also evaluated the effects of the extract on cell membrane integrity and mitochondrial respiration in cultured Hep G2 cells.

RESULTS AND CONCLUSIONS

Water-soluble extracts inhibited (1) oxidation of beta-carotene, (2) AAPH-induced plasma oxidation and (3) Fe(2+)-induced lipid peroxidation in rat liver homogenates. In addition, the extract (4) is an efficient scavenger of the O2- (5) increases intracellular GSH levels and (6) is not cytotoxic. Accordingly, we propose that the therapeutic benefit of Crataegus aronia can be, at least in part, attributed to its effective inhibition of oxidative processes, efficient scavenging of O2- and possible increasing GSH biosynthesis.

Authors+Show Affiliations

Department of Pharmacology, Bruce and Ruth Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, P.O. Box 9649, Haifa 31096, Israel.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15970411

Citation

Ljubuncic, Predrag, et al. "Antioxidant Activity of Crataegus Aronia Aqueous Extract Used in Traditional Arab Medicine in Israel." Journal of Ethnopharmacology, vol. 101, no. 1-3, 2005, pp. 153-61.
Ljubuncic P, Portnaya I, Cogan U, et al. Antioxidant activity of Crataegus aronia aqueous extract used in traditional Arab medicine in Israel. J Ethnopharmacol. 2005;101(1-3):153-61.
Ljubuncic, P., Portnaya, I., Cogan, U., Azaizeh, H., & Bomzon, A. (2005). Antioxidant activity of Crataegus aronia aqueous extract used in traditional Arab medicine in Israel. Journal of Ethnopharmacology, 101(1-3), 153-61.
Ljubuncic P, et al. Antioxidant Activity of Crataegus Aronia Aqueous Extract Used in Traditional Arab Medicine in Israel. J Ethnopharmacol. 2005 Oct 3;101(1-3):153-61. PubMed PMID: 15970411.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant activity of Crataegus aronia aqueous extract used in traditional Arab medicine in Israel. AU - Ljubuncic,Predrag, AU - Portnaya,Irina, AU - Cogan,Uri, AU - Azaizeh,Hassan, AU - Bomzon,Arieh, PY - 2005/01/18/received PY - 2005/04/07/revised PY - 2005/04/14/accepted PY - 2005/6/23/pubmed PY - 2005/12/29/medline PY - 2005/6/23/entrez SP - 153 EP - 61 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 101 IS - 1-3 N2 - BACKGROUND: The medicinal use of extracts prepared from plant parts of the genus Crataegus dates back to ancient times. Furthermore, it has been proposed that its antioxidant constituents account for its beneficial therapeutic effects. A decoction of leaves and unripe fruits from Crataegus aronia syn. azarolus (L) (Rosaceae), the indigenous Israeli hawthorn, is used to treat cardiovascular diseases, cancer, diabetes and sexual weakness in Arab traditional medicine. PURPOSE: Because laboratory data on the bioactivity of extracts prepared from the indigenous Israeli hawthorn is lacking, we evaluated the antioxidant and cytotoxic potentials of an extract prepared from leaves and unripe fruits in a variety of cell and cell-free in vitro assays. METHODS: The antioxidant assays measured: (a) its ability to inhibit (i) oxidation of beta-carotene, (ii) 2,2'-azobis(2-amidino-propan) dihydrochloride (AAPH)-induced plasma oxidation and (iii) iron-induced lipid peroxidation in rat liver homogenates; (b) its ability to scavenge the superoxide (O2-) radical; (c) its effects on the enzyme xanthine oxidase (XO) activity; (d) its effect on the redox state of glutathione (GSH) in cultured Hep G2 cells. In addition, we also evaluated the effects of the extract on cell membrane integrity and mitochondrial respiration in cultured Hep G2 cells. RESULTS AND CONCLUSIONS: Water-soluble extracts inhibited (1) oxidation of beta-carotene, (2) AAPH-induced plasma oxidation and (3) Fe(2+)-induced lipid peroxidation in rat liver homogenates. In addition, the extract (4) is an efficient scavenger of the O2- (5) increases intracellular GSH levels and (6) is not cytotoxic. Accordingly, we propose that the therapeutic benefit of Crataegus aronia can be, at least in part, attributed to its effective inhibition of oxidative processes, efficient scavenging of O2- and possible increasing GSH biosynthesis. SN - 0378-8741 UR - https://www.unboundmedicine.com/medline/citation/15970411/Antioxidant_activity_of_Crataegus_aronia_aqueous_extract_used_in_traditional_Arab_medicine_in_Israel_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(05)00320-X DB - PRIME DP - Unbound Medicine ER -