Tags

Type your tag names separated by a space and hit enter

Antigenicity and immunocrossreactivity of orange tree pollen and orange fruit allergenic extracts.

Abstract

BACKGROUND

It is important to study the crossreactivity between orange tree pollen (OTPE) and orange fruit (OFE) due to the high incidence of pollen/food-related allergies worldwide. The aim of the present study was to determine the antigenic relationship between OTPE and OFE.

METHODS

OTPE and OFErabbit antisera as well as sera from patients allergic to OTPE and OFE were comparatively applied in IgG- and/or IgE-specific ELISA inhibition, crossover or inhibition immunoblotting assays using OTPE and OFE allergenic extracts as solid phase. Periodate and proteinase K treatments were used for carbohydrate and protein depletion, respectively.

RESULTS

The antigenicity of OTPE and the presence of common structures between OTPE and OFE extracts were demonstrated by rabbit IgG-specific ELISA inhibition and crossover immunoblotting assays. A 30-kDa protein, common target of the IgE response on OTPE, OFE and mandarin extract, but absent in lemon extract, was identified by ELISA and immunoblot inhibition assays in patients suffering from primary sensitization to OTPE in the context of occupational exposure. Moreover, biochemical treatments showed that antigenic epitopes on the 30-kDa protein contain polypeptidic but no carbohydrate moieties.

CONCLUSIONS

The antigenicity of OTPE, the presence of common antigenic determinants between pollen and citrus fruits and an IgE-specific crossreactive protein band of 30 kDa sharing carbohydrate-free epitopes were described. After isolation and purification, this common antigen might be useful for allergen immunotherapy in pollen/fruit-related allergic patients.

Links

  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    Centro de Alergia, Hospital de Clínicas, Buenos Aires, Argentina.

    , , , ,

    Source

    MeSH

    Adult
    Allergens
    Citrus sinensis
    Cross Reactions
    Epitopes
    Female
    Food Hypersensitivity
    Fruit
    Humans
    Immunoglobulin E
    Male
    Middle Aged
    Plant Extracts
    Pollen
    Trees

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    15970633

    Citation

    Irañeta, Silvia G., et al. "Antigenicity and Immunocrossreactivity of Orange Tree Pollen and Orange Fruit Allergenic Extracts." International Archives of Allergy and Immunology, vol. 137, no. 4, 2005, pp. 265-72.
    Irañeta SG, Seoane MA, Laucella SA, et al. Antigenicity and immunocrossreactivity of orange tree pollen and orange fruit allergenic extracts. Int Arch Allergy Immunol. 2005;137(4):265-72.
    Irañeta, S. G., Seoane, M. A., Laucella, S. A., Apicella, C., Alonso, A., & Duschak, V. G. (2005). Antigenicity and immunocrossreactivity of orange tree pollen and orange fruit allergenic extracts. International Archives of Allergy and Immunology, 137(4), pp. 265-72.
    Irañeta SG, et al. Antigenicity and Immunocrossreactivity of Orange Tree Pollen and Orange Fruit Allergenic Extracts. Int Arch Allergy Immunol. 2005;137(4):265-72. PubMed PMID: 15970633.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Antigenicity and immunocrossreactivity of orange tree pollen and orange fruit allergenic extracts. AU - Irañeta,Silvia G, AU - Seoane,Marta A, AU - Laucella,Susana A, AU - Apicella,Carolina, AU - Alonso,Angel, AU - Duschak,Vilma G, Y1 - 2005/06/17/ PY - 2004/10/11/received PY - 2005/03/02/accepted PY - 2005/6/23/pubmed PY - 2005/9/28/medline PY - 2005/6/23/entrez SP - 265 EP - 72 JF - International archives of allergy and immunology JO - Int. Arch. Allergy Immunol. VL - 137 IS - 4 N2 - BACKGROUND: It is important to study the crossreactivity between orange tree pollen (OTPE) and orange fruit (OFE) due to the high incidence of pollen/food-related allergies worldwide. The aim of the present study was to determine the antigenic relationship between OTPE and OFE. METHODS: OTPE and OFErabbit antisera as well as sera from patients allergic to OTPE and OFE were comparatively applied in IgG- and/or IgE-specific ELISA inhibition, crossover or inhibition immunoblotting assays using OTPE and OFE allergenic extracts as solid phase. Periodate and proteinase K treatments were used for carbohydrate and protein depletion, respectively. RESULTS: The antigenicity of OTPE and the presence of common structures between OTPE and OFE extracts were demonstrated by rabbit IgG-specific ELISA inhibition and crossover immunoblotting assays. A 30-kDa protein, common target of the IgE response on OTPE, OFE and mandarin extract, but absent in lemon extract, was identified by ELISA and immunoblot inhibition assays in patients suffering from primary sensitization to OTPE in the context of occupational exposure. Moreover, biochemical treatments showed that antigenic epitopes on the 30-kDa protein contain polypeptidic but no carbohydrate moieties. CONCLUSIONS: The antigenicity of OTPE, the presence of common antigenic determinants between pollen and citrus fruits and an IgE-specific crossreactive protein band of 30 kDa sharing carbohydrate-free epitopes were described. After isolation and purification, this common antigen might be useful for allergen immunotherapy in pollen/fruit-related allergic patients. SN - 1018-2438 UR - https://www.unboundmedicine.com/medline/citation/15970633/Antigenicity_and_immunocrossreactivity_of_orange_tree_pollen_and_orange_fruit_allergenic_extracts_ L2 - https://www.karger.com?DOI=10.1159/000086419 DB - PRIME DP - Unbound Medicine ER -