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Purification and determination of plant hormones auxin and abscisic acid using solid phase extraction and two-dimensional high performance liquid chromatography.
J Chromatogr A. 2005 May 20; 1075(1-2):159-66.JC

Abstract

A method for separation and purification of plant hormones auxin and abscisic acid based on mixed mode reversed-phase anion-exchange solid phase extraction and two-dimensional HPLC was developed. Two-dimensional HPLC in "heart cutting" mode was very efficient in the purification of these two hormones. Its purification power is high enough to allow reliable on-line quantification of both hormones even with non-selective detectors.

Authors+Show Affiliations

Dobrev PI
Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 135, 16502 Praha 6, Czech Republic. dobrev@ueb.cas.cz
Havlícek L
No affiliation info available
Vagner M
No affiliation info available
Malbeck J
No affiliation info available
Kamínek M
No affiliation info available

MeSH

Abscisic AcidChromatography, High Pressure LiquidIndoleacetic AcidsPlant Growth RegulatorsReproducibility of Results

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15974129

Citation

Dobrev, P I., et al. "Purification and Determination of Plant Hormones Auxin and Abscisic Acid Using Solid Phase Extraction and Two-dimensional High Performance Liquid Chromatography." Journal of Chromatography. A, vol. 1075, no. 1-2, 2005, pp. 159-66.
Dobrev PI, Havlícek L, Vagner M, et al. Purification and determination of plant hormones auxin and abscisic acid using solid phase extraction and two-dimensional high performance liquid chromatography. J Chromatogr A. 2005;1075(1-2):159-66.
Dobrev, P. I., Havlícek, L., Vagner, M., Malbeck, J., & Kamínek, M. (2005). Purification and determination of plant hormones auxin and abscisic acid using solid phase extraction and two-dimensional high performance liquid chromatography. Journal of Chromatography. A, 1075(1-2), 159-66.
Dobrev PI, et al. Purification and Determination of Plant Hormones Auxin and Abscisic Acid Using Solid Phase Extraction and Two-dimensional High Performance Liquid Chromatography. J Chromatogr A. 2005 May 20;1075(1-2):159-66. PubMed PMID: 15974129.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Purification and determination of plant hormones auxin and abscisic acid using solid phase extraction and two-dimensional high performance liquid chromatography. AU - Dobrev,P I, AU - Havlícek,L, AU - Vagner,M, AU - Malbeck,J, AU - Kamínek,M, PY - 2005/6/25/pubmed PY - 2005/9/30/medline PY - 2005/6/25/entrez SP - 159 EP - 66 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1075 IS - 1-2 N2 - A method for separation and purification of plant hormones auxin and abscisic acid based on mixed mode reversed-phase anion-exchange solid phase extraction and two-dimensional HPLC was developed. Two-dimensional HPLC in "heart cutting" mode was very efficient in the purification of these two hormones. Its purification power is high enough to allow reliable on-line quantification of both hormones even with non-selective detectors. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/15974129/Purification_and_determination_of_plant_hormones_auxin_and_abscisic_acid_using_solid_phase_extraction_and_two_dimensional_high_performance_liquid_chromatography_ DB - PRIME DP - Unbound Medicine ER -