Tags

Type your tag names separated by a space and hit enter

Salacia oblonga root improves postprandial hyperlipidemia and hepatic steatosis in Zucker diabetic fatty rats: activation of PPAR-alpha.
Toxicol Appl Pharmacol. 2006 Feb 01; 210(3):225-35.TA

Abstract

Salacia oblonga (SO) root is an Ayurvedic medicine with anti-diabetic and anti-obese properties. Peroxisome proliferator-activated receptor (PPAR)-alpha, a nuclear receptor, plays an important role in maintaining the homeostasis of lipid metabolism. Here, we demonstrate that chronic oral administration of the water extract from the root of SO to Zucker diabetic fatty (ZDF) rats, a genetic model of type 2 diabetes and obesity, lowered plasma triglyceride and total cholesterol (TC) levels, increased plasma high-density lipoprotein levels and reduced the liver contents of triglyceride, non-esterified fatty acids (NEFA) and the ratio of fatty droplets to total tissue. By contrast, the extract had no effect on plasma triglyceride and TC levels in fasted ZDF rats. After olive oil administration to ZDF the extract also inhibited the increase in plasma triglyceride levels. These results suggest that SO extract improves postprandial hyperlipidemia and hepatic steatosis in ZDF rats. Additionally, SO treatment enhanced hepatic expression of PPAR-alpha mRNA and protein, and carnitine palmitoyltransferase-1 and acyl-CoA oxidase mRNAs in ZDF rats. In vitro, SO extract and its main component mangiferin activated PPAR-alpha luciferase activity in human embryonic kidney 293 cells and lipoprotein lipase mRNA expression and enzyme activity in THP-1 differentiated macrophages; these effects were completely suppressed by a selective PPAR-alpha antagonist MK-886. The findings from both in vivo and in vitro suggest that SO extract functions as a PPAR-alpha activator, providing a potential mechanism for improvement of postprandial hyperlipidemia and hepatic steatosis in diabetes and obesity.

Authors+Show Affiliations

Herbal Medicines Research and Education Centre, Faculty of Pharmacy, The University of Sydney, NSW 2006, Australia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15975614

Citation

Huang, Tom Hsun-Wei, et al. "Salacia Oblonga Root Improves Postprandial Hyperlipidemia and Hepatic Steatosis in Zucker Diabetic Fatty Rats: Activation of PPAR-alpha." Toxicology and Applied Pharmacology, vol. 210, no. 3, 2006, pp. 225-35.
Huang TH, Peng G, Li GQ, et al. Salacia oblonga root improves postprandial hyperlipidemia and hepatic steatosis in Zucker diabetic fatty rats: activation of PPAR-alpha. Toxicol Appl Pharmacol. 2006;210(3):225-35.
Huang, T. H., Peng, G., Li, G. Q., Yamahara, J., Roufogalis, B. D., & Li, Y. (2006). Salacia oblonga root improves postprandial hyperlipidemia and hepatic steatosis in Zucker diabetic fatty rats: activation of PPAR-alpha. Toxicology and Applied Pharmacology, 210(3), 225-35.
Huang TH, et al. Salacia Oblonga Root Improves Postprandial Hyperlipidemia and Hepatic Steatosis in Zucker Diabetic Fatty Rats: Activation of PPAR-alpha. Toxicol Appl Pharmacol. 2006 Feb 1;210(3):225-35. PubMed PMID: 15975614.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Salacia oblonga root improves postprandial hyperlipidemia and hepatic steatosis in Zucker diabetic fatty rats: activation of PPAR-alpha. AU - Huang,Tom Hsun-Wei, AU - Peng,Gang, AU - Li,George Qian, AU - Yamahara,Johji, AU - Roufogalis,Basil D, AU - Li,Yuhao, Y1 - 2005/06/21/ PY - 2005/01/20/received PY - 2005/05/04/revised PY - 2005/05/09/accepted PY - 2005/6/25/pubmed PY - 2006/3/17/medline PY - 2005/6/25/entrez SP - 225 EP - 35 JF - Toxicology and applied pharmacology JO - Toxicol. Appl. Pharmacol. VL - 210 IS - 3 N2 - Salacia oblonga (SO) root is an Ayurvedic medicine with anti-diabetic and anti-obese properties. Peroxisome proliferator-activated receptor (PPAR)-alpha, a nuclear receptor, plays an important role in maintaining the homeostasis of lipid metabolism. Here, we demonstrate that chronic oral administration of the water extract from the root of SO to Zucker diabetic fatty (ZDF) rats, a genetic model of type 2 diabetes and obesity, lowered plasma triglyceride and total cholesterol (TC) levels, increased plasma high-density lipoprotein levels and reduced the liver contents of triglyceride, non-esterified fatty acids (NEFA) and the ratio of fatty droplets to total tissue. By contrast, the extract had no effect on plasma triglyceride and TC levels in fasted ZDF rats. After olive oil administration to ZDF the extract also inhibited the increase in plasma triglyceride levels. These results suggest that SO extract improves postprandial hyperlipidemia and hepatic steatosis in ZDF rats. Additionally, SO treatment enhanced hepatic expression of PPAR-alpha mRNA and protein, and carnitine palmitoyltransferase-1 and acyl-CoA oxidase mRNAs in ZDF rats. In vitro, SO extract and its main component mangiferin activated PPAR-alpha luciferase activity in human embryonic kidney 293 cells and lipoprotein lipase mRNA expression and enzyme activity in THP-1 differentiated macrophages; these effects were completely suppressed by a selective PPAR-alpha antagonist MK-886. The findings from both in vivo and in vitro suggest that SO extract functions as a PPAR-alpha activator, providing a potential mechanism for improvement of postprandial hyperlipidemia and hepatic steatosis in diabetes and obesity. SN - 0041-008X UR - https://www.unboundmedicine.com/medline/citation/15975614/Salacia_oblonga_root_improves_postprandial_hyperlipidemia_and_hepatic_steatosis_in_Zucker_diabetic_fatty_rats:_activation_of_PPAR_alpha_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-008X(05)00219-X DB - PRIME DP - Unbound Medicine ER -