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[Effects of nifedipine on myosin heavy chain (MHC) isoforms transition in unloaded soleus].
Space Med Med Eng (Beijing). 2005 Apr; 18(2):89-93.SM

Abstract

OBJECTIVE

To observe the effects of nifedipine, a blocker of L-type Ca2+ channel, on soleus weight and expression of myosin heavy chain (MHC) isoforms in control and tail-suspended rats.

METHOD

Animals were treated with nifedipine at a dose of 10 mg/kg per day in drinking water for 1 or 2 weeks. The expression of MHC isoform protein was observed by SDS-polyacrylamide gel electrophoresis at 4 degrees C. The expression of MHC mRNA was detected by RT-PCR.

RESULT

The relative weight (muscle weight/body weight) of soleus muscle was decreased by 39.5% and 51.7% in 1 and 2 weeks of tail-suspended group respectively, but no changes in extensor digitorum longus (EDL) weight was found. The relative weight of soleus and EDL in 1 or 2 weeks of nifedipine treated control group showed no changes as compared with the untreated control group. The relative weight of soleus in 1 or 2 weeks of nifedipine treated tail-suspension group decreased by 36.6% or 52.0%, respectively, as compared with control, but there was no difference between tail-suspension group with or without nifedipine treatment. The expression of MHC I, IIa mRNA and protein could be detected in control soleus with or without nifedipine treatment. The expression MHC I, IIa, IIx and IIb mRNA was detected in 1 or 2 weeks of unloaded soleus with or without nifedipine treatment. Nifedipine inhibited the expression of II type MHC mRNA in unloaded soleus and expression of MHC IIa protein in control and unloaded soleus.

CONCLUSION

Nifedipine can not resist atrophy of unloaded soleus, but inhibit the transition of MHC from slow to fast isoforms at the transcription level.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

15977384

Citation

Feng, Han-zhong, and Zhi-bin Yu. "[Effects of Nifedipine On Myosin Heavy Chain (MHC) Isoforms Transition in Unloaded Soleus]." Hang Tian Yi Xue Yu Yi Xue Gong Cheng = Space Medicine & Medical Engineering, vol. 18, no. 2, 2005, pp. 89-93.
Feng HZ, Yu ZB. [Effects of nifedipine on myosin heavy chain (MHC) isoforms transition in unloaded soleus]. Space Med Med Eng (Beijing). 2005;18(2):89-93.
Feng, H. Z., & Yu, Z. B. (2005). [Effects of nifedipine on myosin heavy chain (MHC) isoforms transition in unloaded soleus]. Hang Tian Yi Xue Yu Yi Xue Gong Cheng = Space Medicine & Medical Engineering, 18(2), 89-93.
Feng HZ, Yu ZB. [Effects of Nifedipine On Myosin Heavy Chain (MHC) Isoforms Transition in Unloaded Soleus]. Space Med Med Eng (Beijing). 2005;18(2):89-93. PubMed PMID: 15977384.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of nifedipine on myosin heavy chain (MHC) isoforms transition in unloaded soleus]. AU - Feng,Han-zhong, AU - Yu,Zhi-bin, PY - 2005/6/28/pubmed PY - 2005/8/9/medline PY - 2005/6/28/entrez SP - 89 EP - 93 JF - Hang tian yi xue yu yi xue gong cheng = Space medicine & medical engineering JO - Space Med Med Eng (Beijing) VL - 18 IS - 2 N2 - OBJECTIVE: To observe the effects of nifedipine, a blocker of L-type Ca2+ channel, on soleus weight and expression of myosin heavy chain (MHC) isoforms in control and tail-suspended rats. METHOD: Animals were treated with nifedipine at a dose of 10 mg/kg per day in drinking water for 1 or 2 weeks. The expression of MHC isoform protein was observed by SDS-polyacrylamide gel electrophoresis at 4 degrees C. The expression of MHC mRNA was detected by RT-PCR. RESULT: The relative weight (muscle weight/body weight) of soleus muscle was decreased by 39.5% and 51.7% in 1 and 2 weeks of tail-suspended group respectively, but no changes in extensor digitorum longus (EDL) weight was found. The relative weight of soleus and EDL in 1 or 2 weeks of nifedipine treated control group showed no changes as compared with the untreated control group. The relative weight of soleus in 1 or 2 weeks of nifedipine treated tail-suspension group decreased by 36.6% or 52.0%, respectively, as compared with control, but there was no difference between tail-suspension group with or without nifedipine treatment. The expression of MHC I, IIa mRNA and protein could be detected in control soleus with or without nifedipine treatment. The expression MHC I, IIa, IIx and IIb mRNA was detected in 1 or 2 weeks of unloaded soleus with or without nifedipine treatment. Nifedipine inhibited the expression of II type MHC mRNA in unloaded soleus and expression of MHC IIa protein in control and unloaded soleus. CONCLUSION: Nifedipine can not resist atrophy of unloaded soleus, but inhibit the transition of MHC from slow to fast isoforms at the transcription level. SN - 1002-0837 UR - https://www.unboundmedicine.com/medline/citation/15977384/[Effects_of_nifedipine_on_myosin_heavy_chain__MHC__isoforms_transition_in_unloaded_soleus]_ L2 - https://antibodies.cancer.gov/detail/CPTC-HLA-C-1 DB - PRIME DP - Unbound Medicine ER -