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fldA is an essential gene required in the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis.
FEBS Lett. 2005 Jul 04; 579(17):3802-6.FL

Abstract

Although flavodoxin I is indispensable for Escherichia coli growth, the exact pathway(s) where flavodoxin I is essential has not been identified. We performed transposon mutagenesis of the flavodoxin I gene, fldA, in an E. coli strain that expressed mevalonate pathway enzymes and that had a point mutation in the lytB gene of the MEP pathway resulting in the accumulation of (E)-4-hydroxy-3-methylbutyl-2-enyl pyrophosphate (HMBPP). Disruption of fldA abrogated mevalonate-independent growth and dramatically decreased HMBPP levels. The fldA- mutant grew with mevalonate indicating that the essential role of flavodoxin I under aerobic conditions is in the MEP pathway. Growth was restored by fldA complementation. Since GcpE (which synthesizes HMBPP) and LytB are iron-sulfur enzymes that require a reducing system for their activity, we propose that flavodoxin is essential for GcpE and possibly LytB activity. Thus, the essential role for flavodoxin I in E. coli is in the MEP pathway for isoprenoid biosynthesis.

Authors+Show Affiliations

Puan KJ
Division of Rheumatology, Department of Internal Medicine and the Interdisciplinary Group in Immunology, University of Iowa, EMRB 340F, Iowa City, IA 52242, USA.
Wang H
No affiliation info available
Dairi T
No affiliation info available
Kuzuyama T
No affiliation info available
Morita CT
No affiliation info available

MeSH

Amino Acid SubstitutionAspartic AcidErythritolEscherichia coliEscherichia coli ProteinsFlavodoxinGenes, EssentialGenetic Complementation TestGlycineMutagenesisSugar PhosphatesTerpenes

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15978585

Citation

Puan, Kia-Joo, et al. "FldA Is an Essential Gene Required in the 2-C-methyl-D-erythritol 4-phosphate Pathway for Isoprenoid Biosynthesis." FEBS Letters, vol. 579, no. 17, 2005, pp. 3802-6.
Puan KJ, Wang H, Dairi T, et al. FldA is an essential gene required in the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis. FEBS Lett. 2005;579(17):3802-6.
Puan, K. J., Wang, H., Dairi, T., Kuzuyama, T., & Morita, C. T. (2005). FldA is an essential gene required in the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis. FEBS Letters, 579(17), 3802-6.
Puan KJ, et al. FldA Is an Essential Gene Required in the 2-C-methyl-D-erythritol 4-phosphate Pathway for Isoprenoid Biosynthesis. FEBS Lett. 2005 Jul 4;579(17):3802-6. PubMed PMID: 15978585.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - fldA is an essential gene required in the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis. AU - Puan,Kia-Joo, AU - Wang,Hong, AU - Dairi,Tohru, AU - Kuzuyama,Tomohisa, AU - Morita,Craig T, PY - 2005/04/02/received PY - 2005/05/24/revised PY - 2005/05/25/accepted PY - 2005/6/28/pubmed PY - 2005/9/2/medline PY - 2005/6/28/entrez SP - 3802 EP - 6 JF - FEBS letters JO - FEBS Lett VL - 579 IS - 17 N2 - Although flavodoxin I is indispensable for Escherichia coli growth, the exact pathway(s) where flavodoxin I is essential has not been identified. We performed transposon mutagenesis of the flavodoxin I gene, fldA, in an E. coli strain that expressed mevalonate pathway enzymes and that had a point mutation in the lytB gene of the MEP pathway resulting in the accumulation of (E)-4-hydroxy-3-methylbutyl-2-enyl pyrophosphate (HMBPP). Disruption of fldA abrogated mevalonate-independent growth and dramatically decreased HMBPP levels. The fldA- mutant grew with mevalonate indicating that the essential role of flavodoxin I under aerobic conditions is in the MEP pathway. Growth was restored by fldA complementation. Since GcpE (which synthesizes HMBPP) and LytB are iron-sulfur enzymes that require a reducing system for their activity, we propose that flavodoxin is essential for GcpE and possibly LytB activity. Thus, the essential role for flavodoxin I in E. coli is in the MEP pathway for isoprenoid biosynthesis. SN - 0014-5793 UR - https://www.unboundmedicine.com/medline/citation/15978585/fldA_is_an_essential_gene_required_in_the_2_C_methyl_D_erythritol_4_phosphate_pathway_for_isoprenoid_biosynthesis_ DB - PRIME DP - Unbound Medicine ER -