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The saponin monomer of dwarf lilyturf tuber, DT-13, reduces L-type calcium currents during hypoxia in adult rat ventricular myocytes.
Life Sci. 2005 Oct 28; 77(24):3021-30.LS

Abstract

The saponin monomer 13 of dwarf lilyturf tuber (DT-13), one of the saponin monomers of dwarf lilyturf tuber, has been found to have potent cardioprotective effects. In order to investigate the effects of DT-13 on L-type calcium currents (I(Ca,L)), exploring the mechanisms of DT-13's cardioprotective effects in the condition of pathophysiology, we directly measured the I(Ca,L) during hypoxia in the adult rat cardiac myocytes exposed to DT-13 using standard whole-cell patch-clamp recording technique. Our previous results showed that DT-13 exerted decreasing effects on the I(Ca,L) of the single adult rat cardiac myocytes. In the condition of hypoxia, the current density was inhibited by about 29% after exposure of the cells to DT-13 (0.1 micromol L(-1)) for 10 min, from 6.96+/-1.05 pA/pF to 4.38+/-0.35 pA/pF (n=5, P<0.05). This I(Ca,L)-inhibiting action of DT-13 was concentration-dependent and showed no frequency-dependence. DT-13 up-shifted the current-voltage (I-V) curve. Steady-state activation of I(Ca,L) was not affected markedly, and the half activation potential (V(0.5)) in the presence of DT-13 (0.1 micromol L(-1)) was also not significantly different. DT-13 at 0.1 micromol L(-1) markedly accelerated the voltage-dependent steady-state inactivation of calcium current and shifted the steady-state inactivation curve of I(Ca,L) to the left. In combination with previous reports, these results suggest that there might be a close relationship between the cardioprotective effects of DT-13 and L-type calcium channels in the condition of hypoxia.

Authors+Show Affiliations

Tao J
Department of Pharmacology, Nanjing Medical University, Hanzhong Road 140, Nanjing 210029, PR China.
Wang H
No affiliation info available
Zhou H
No affiliation info available
Li S
No affiliation info available

MeSH

AnimalsCalcium Channel BlockersCalcium Channels, L-TypeCell HypoxiaDose-Response Relationship, DrugFemaleHeart VentriclesMaleMedicine, Chinese TraditionalMembrane PotentialsMyocytes, CardiacOphiopogonPatch-Clamp TechniquesPlant PreparationsRatsRats, Sprague-DawleySaponinsVentricular Function

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15978637

Citation

Tao, Jin, et al. "The Saponin Monomer of Dwarf Lilyturf Tuber, DT-13, Reduces L-type Calcium Currents During Hypoxia in Adult Rat Ventricular Myocytes." Life Sciences, vol. 77, no. 24, 2005, pp. 3021-30.
Tao J, Wang H, Zhou H, et al. The saponin monomer of dwarf lilyturf tuber, DT-13, reduces L-type calcium currents during hypoxia in adult rat ventricular myocytes. Life Sci. 2005;77(24):3021-30.
Tao, J., Wang, H., Zhou, H., & Li, S. (2005). The saponin monomer of dwarf lilyturf tuber, DT-13, reduces L-type calcium currents during hypoxia in adult rat ventricular myocytes. Life Sciences, 77(24), 3021-30.
Tao J, et al. The Saponin Monomer of Dwarf Lilyturf Tuber, DT-13, Reduces L-type Calcium Currents During Hypoxia in Adult Rat Ventricular Myocytes. Life Sci. 2005 Oct 28;77(24):3021-30. PubMed PMID: 15978637.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The saponin monomer of dwarf lilyturf tuber, DT-13, reduces L-type calcium currents during hypoxia in adult rat ventricular myocytes. AU - Tao,Jin, AU - Wang,Hongyi, AU - Zhou,Hong, AU - Li,Shengnan, Y1 - 2005/06/23/ PY - 2004/07/12/received PY - 2005/01/29/accepted PY - 2005/6/28/pubmed PY - 2005/11/16/medline PY - 2005/6/28/entrez SP - 3021 EP - 30 JF - Life sciences JO - Life Sci VL - 77 IS - 24 N2 - The saponin monomer 13 of dwarf lilyturf tuber (DT-13), one of the saponin monomers of dwarf lilyturf tuber, has been found to have potent cardioprotective effects. In order to investigate the effects of DT-13 on L-type calcium currents (I(Ca,L)), exploring the mechanisms of DT-13's cardioprotective effects in the condition of pathophysiology, we directly measured the I(Ca,L) during hypoxia in the adult rat cardiac myocytes exposed to DT-13 using standard whole-cell patch-clamp recording technique. Our previous results showed that DT-13 exerted decreasing effects on the I(Ca,L) of the single adult rat cardiac myocytes. In the condition of hypoxia, the current density was inhibited by about 29% after exposure of the cells to DT-13 (0.1 micromol L(-1)) for 10 min, from 6.96+/-1.05 pA/pF to 4.38+/-0.35 pA/pF (n=5, P<0.05). This I(Ca,L)-inhibiting action of DT-13 was concentration-dependent and showed no frequency-dependence. DT-13 up-shifted the current-voltage (I-V) curve. Steady-state activation of I(Ca,L) was not affected markedly, and the half activation potential (V(0.5)) in the presence of DT-13 (0.1 micromol L(-1)) was also not significantly different. DT-13 at 0.1 micromol L(-1) markedly accelerated the voltage-dependent steady-state inactivation of calcium current and shifted the steady-state inactivation curve of I(Ca,L) to the left. In combination with previous reports, these results suggest that there might be a close relationship between the cardioprotective effects of DT-13 and L-type calcium channels in the condition of hypoxia. SN - 0024-3205 UR - https://www.unboundmedicine.com/medline/citation/15978637/The_saponin_monomer_of_dwarf_lilyturf_tuber_DT_13_reduces_L_type_calcium_currents_during_hypoxia_in_adult_rat_ventricular_myocytes_ DB - PRIME DP - Unbound Medicine ER -