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Association of the osteoprotegerin gene polymorphisms with bone mineral density in postmenopausal women.
Maturitas. 2005 Jul 16; 51(3):270-9.M

Abstract

OBJECTIVES

Osteoprotegerin (OPG) is a recently discovered member of the tumour necrosis factor receptor superfamily. It plays a crucial role in the control of bone resorption and its gene could therefore be a good candidate gene for osteoporosis. The aim of our work was to find polymorphisms in the OPG gene and to investigate their possible contribution to the genetic susceptibility to osteoporosis by testing for their association with bone mineral density (BMD).

METHODS

The whole OPG gene coding region was screened for the presence of polymorphisms in a group of 60 osteoporotic women by single-strand conformation polymorphism analysis (SSCP) approach. Association of the discovered polymorphisms with bone mineral density was investigated in 136 Slovenian postmenopausal women.

RESULTS

We detected eight OPG gene polymorphisms that were confirmed by direct DNA sequencing, deletion 4752_4753delCT and nucleotide substitutions 1181G>C, 1217C>T, 1284G>A, 4501C>T, 6893A>G, 6950A>C and 8738T>A. Nucleotide substitutions 1284G>A and 8738T>A have not been previously described. Polymorphisms 4752_4753delCT, 6893A>G and 6950A>C were in complete linkage and the same was true for 1217C>T and 4501C>T. The association with BMD was found only for polymorphism 1181G>C. Subjects with genotype 1181GG had significantly lower lumbar spine BMD than subjects displaying 1181GC.

CONCLUSIONS

By our approach we detected eight polymorphisms in the OPG gene. According to our analysis polymorphism 1181G>C is associated with BMD and could therefore be considered as an element of genetic susceptibility to osteoporosis.

Authors+Show Affiliations

Department of Clinical Biochemistry, Faculty of Pharmacy, Askerceva 7, SI-1000 Ljubljana, Slovenia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15978970

Citation

Arko, Barbara, et al. "Association of the Osteoprotegerin Gene Polymorphisms With Bone Mineral Density in Postmenopausal Women." Maturitas, vol. 51, no. 3, 2005, pp. 270-9.
Arko B, Prezelj J, Kocijancic A, et al. Association of the osteoprotegerin gene polymorphisms with bone mineral density in postmenopausal women. Maturitas. 2005;51(3):270-9.
Arko, B., Prezelj, J., Kocijancic, A., Komel, R., & Marc, J. (2005). Association of the osteoprotegerin gene polymorphisms with bone mineral density in postmenopausal women. Maturitas, 51(3), 270-9.
Arko B, et al. Association of the Osteoprotegerin Gene Polymorphisms With Bone Mineral Density in Postmenopausal Women. Maturitas. 2005 Jul 16;51(3):270-9. PubMed PMID: 15978970.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Association of the osteoprotegerin gene polymorphisms with bone mineral density in postmenopausal women. AU - Arko,Barbara, AU - Prezelj,Janez, AU - Kocijancic,Andreja, AU - Komel,Radovan, AU - Marc,Janja, PY - 2004/03/29/received PY - 2004/08/11/revised PY - 2004/08/12/accepted PY - 2005/6/28/pubmed PY - 2006/1/6/medline PY - 2005/6/28/entrez SP - 270 EP - 9 JF - Maturitas JO - Maturitas VL - 51 IS - 3 N2 - OBJECTIVES: Osteoprotegerin (OPG) is a recently discovered member of the tumour necrosis factor receptor superfamily. It plays a crucial role in the control of bone resorption and its gene could therefore be a good candidate gene for osteoporosis. The aim of our work was to find polymorphisms in the OPG gene and to investigate their possible contribution to the genetic susceptibility to osteoporosis by testing for their association with bone mineral density (BMD). METHODS: The whole OPG gene coding region was screened for the presence of polymorphisms in a group of 60 osteoporotic women by single-strand conformation polymorphism analysis (SSCP) approach. Association of the discovered polymorphisms with bone mineral density was investigated in 136 Slovenian postmenopausal women. RESULTS: We detected eight OPG gene polymorphisms that were confirmed by direct DNA sequencing, deletion 4752_4753delCT and nucleotide substitutions 1181G>C, 1217C>T, 1284G>A, 4501C>T, 6893A>G, 6950A>C and 8738T>A. Nucleotide substitutions 1284G>A and 8738T>A have not been previously described. Polymorphisms 4752_4753delCT, 6893A>G and 6950A>C were in complete linkage and the same was true for 1217C>T and 4501C>T. The association with BMD was found only for polymorphism 1181G>C. Subjects with genotype 1181GG had significantly lower lumbar spine BMD than subjects displaying 1181GC. CONCLUSIONS: By our approach we detected eight polymorphisms in the OPG gene. According to our analysis polymorphism 1181G>C is associated with BMD and could therefore be considered as an element of genetic susceptibility to osteoporosis. SN - 0378-5122 UR - https://www.unboundmedicine.com/medline/citation/15978970/Association_of_the_osteoprotegerin_gene_polymorphisms_with_bone_mineral_density_in_postmenopausal_women_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-5122(04)00274-9 DB - PRIME DP - Unbound Medicine ER -