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Differential expression of two cytochrome P450 genes in compatible and incompatible Hessian fly/wheat interactions.
Insect Biochem Mol Biol. 2005 Sep; 35(9):981-9.IB

Abstract

We have recovered two Hessian fly cytochrome P450 cDNAs from an ongoing midgut EST project. CYP6AZ1 and CYP6BA1 represent two new subfamilies within the CYP6 family. The deduced amino acid sequences for CYP6AZ1 and CYP6BA1 show conserved structural and functional domains of insect P450s. Expression analysis with reverse transcription-polymerase chain reaction (RT-PCR) indicated that CYP6AZ1 is midgut specific and induced during active larval feeding, whereas CYP6BA1 was expressed in all tissues and developmental stages examined. Further expression analysis of CYP6AZ1 with RT-PCR in compatible and incompatible Hessian fly/wheat interactions suggested that CYP6AZ1 may be required for larval feeding in compatible interactions. These results should lead to a better understanding of the Hessian fly/wheat interaction with emphasis on the larval midgut as a critical interface with its host plant.

Authors+Show Affiliations

Department of Entomology, Purdue University, West Lafayette, IN, USA. omitt@purdue.eduNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

15978999

Citation

Mittapalli, Omprakash, et al. "Differential Expression of Two Cytochrome P450 Genes in Compatible and Incompatible Hessian Fly/wheat Interactions." Insect Biochemistry and Molecular Biology, vol. 35, no. 9, 2005, pp. 981-9.
Mittapalli O, Neal JJ, Shukle RH. Differential expression of two cytochrome P450 genes in compatible and incompatible Hessian fly/wheat interactions. Insect Biochem Mol Biol. 2005;35(9):981-9.
Mittapalli, O., Neal, J. J., & Shukle, R. H. (2005). Differential expression of two cytochrome P450 genes in compatible and incompatible Hessian fly/wheat interactions. Insect Biochemistry and Molecular Biology, 35(9), 981-9.
Mittapalli O, Neal JJ, Shukle RH. Differential Expression of Two Cytochrome P450 Genes in Compatible and Incompatible Hessian Fly/wheat Interactions. Insect Biochem Mol Biol. 2005;35(9):981-9. PubMed PMID: 15978999.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential expression of two cytochrome P450 genes in compatible and incompatible Hessian fly/wheat interactions. AU - Mittapalli,Omprakash, AU - Neal,Jonathan J, AU - Shukle,Richard H, Y1 - 2005/04/26/ PY - 2005/01/18/received PY - 2005/03/31/revised PY - 2005/03/31/accepted PY - 2005/6/28/pubmed PY - 2005/8/25/medline PY - 2005/6/28/entrez SP - 981 EP - 9 JF - Insect biochemistry and molecular biology JO - Insect Biochem Mol Biol VL - 35 IS - 9 N2 - We have recovered two Hessian fly cytochrome P450 cDNAs from an ongoing midgut EST project. CYP6AZ1 and CYP6BA1 represent two new subfamilies within the CYP6 family. The deduced amino acid sequences for CYP6AZ1 and CYP6BA1 show conserved structural and functional domains of insect P450s. Expression analysis with reverse transcription-polymerase chain reaction (RT-PCR) indicated that CYP6AZ1 is midgut specific and induced during active larval feeding, whereas CYP6BA1 was expressed in all tissues and developmental stages examined. Further expression analysis of CYP6AZ1 with RT-PCR in compatible and incompatible Hessian fly/wheat interactions suggested that CYP6AZ1 may be required for larval feeding in compatible interactions. These results should lead to a better understanding of the Hessian fly/wheat interaction with emphasis on the larval midgut as a critical interface with its host plant. SN - 0965-1748 UR - https://www.unboundmedicine.com/medline/citation/15978999/Differential_expression_of_two_cytochrome_P450_genes_in_compatible_and_incompatible_Hessian_fly/wheat_interactions_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0965-1748(05)00100-1 DB - PRIME DP - Unbound Medicine ER -