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Biomonitoring of ciguatoxin exposure in mice using blood collection cards.
Toxicon. 2005 Sep 01; 46(3):243-51.T

Abstract

Ciguatera is a human food poisoning caused by consumption of tropical and subtropical fish that have, through their diet, accumulated ciguatoxins in their tissues. This study used laboratory mice to investigate the potential to apply blood collection cards to biomonitor ciguatoxin exposure. Quantitation by the neuroblastoma cytotoxicity assay of Caribbean ciguatoxin (C-CTX-1) spiked into mice blood was made with good precision and recovery. The blood collected from mice exposed to a sublethal dose of Caribbean ciguatoxic extract (0.59 ng/g C-CTX-1 equivalents) was analyzed and found to contain detectable toxin levels at least 12 h post-exposure. Calculated concentration varied from 0.25 ng/ml at 30 min post-exposure to 0.12 ng/ml at 12 h. A dose response mice exposure revealed a linear dose-dependent increase of ciguatoxin activity in mice blood, with more polar ciguatoxin congeners contributing to 89% of the total toxicity. Finally, the toxin measurement in mice blood exposed to toxic extracts from the Indian Ocean or from the Pacific Ocean showed that the blood collection card method could be extended to each of the three known ciguatoxin families (C-CTX, I-CTX and P-CTX). The low matrix effect of extracted dried-blood samples (used at 1:10 or 1:20 dilution) and the high sensitivity of the neuroblastoma assay (limit of detection 0.006 ng/ml C-CTX-1), determined that the blood collection card method is suitable to monitor ciguatoxin at sublethal doses in mice and opens the potential to be a useful procedure for fish screening, environmental risk assessment or clinical diagnosis of ciguatera fish poisoning in humans or marine mammals.

Authors+Show Affiliations

Marine Biotoxins Program, Center for Coastal Environmental Health and Biomolecular Research, NOAA-National Ocean Service, Coastal Research Branch, 219 Fort Johnson Road, Charleston, SC 29412, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15979117

Citation

Bottein Dechraoui, M-Yasmine, et al. "Biomonitoring of Ciguatoxin Exposure in Mice Using Blood Collection Cards." Toxicon : Official Journal of the International Society On Toxinology, vol. 46, no. 3, 2005, pp. 243-51.
Bottein Dechraoui MY, Wang Z, Turquet J, et al. Biomonitoring of ciguatoxin exposure in mice using blood collection cards. Toxicon. 2005;46(3):243-51.
Bottein Dechraoui, M. Y., Wang, Z., Turquet, J., Chinain, M., Darius, T., Cruchet, P., Radwan, F. F., Dickey, R. W., & Ramsdell, J. S. (2005). Biomonitoring of ciguatoxin exposure in mice using blood collection cards. Toxicon : Official Journal of the International Society On Toxinology, 46(3), 243-51.
Bottein Dechraoui MY, et al. Biomonitoring of Ciguatoxin Exposure in Mice Using Blood Collection Cards. Toxicon. 2005 Sep 1;46(3):243-51. PubMed PMID: 15979117.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biomonitoring of ciguatoxin exposure in mice using blood collection cards. AU - Bottein Dechraoui,M-Yasmine, AU - Wang,Zhihong, AU - Turquet,Jean, AU - Chinain,Mireille, AU - Darius,Taiana, AU - Cruchet,Philippe, AU - Radwan,Faisal F Y, AU - Dickey,Robert W, AU - Ramsdell,John S, PY - 2004/12/03/received PY - 2005/03/10/revised PY - 2005/03/11/accepted PY - 2005/6/28/pubmed PY - 2005/10/20/medline PY - 2005/6/28/entrez SP - 243 EP - 51 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 46 IS - 3 N2 - Ciguatera is a human food poisoning caused by consumption of tropical and subtropical fish that have, through their diet, accumulated ciguatoxins in their tissues. This study used laboratory mice to investigate the potential to apply blood collection cards to biomonitor ciguatoxin exposure. Quantitation by the neuroblastoma cytotoxicity assay of Caribbean ciguatoxin (C-CTX-1) spiked into mice blood was made with good precision and recovery. The blood collected from mice exposed to a sublethal dose of Caribbean ciguatoxic extract (0.59 ng/g C-CTX-1 equivalents) was analyzed and found to contain detectable toxin levels at least 12 h post-exposure. Calculated concentration varied from 0.25 ng/ml at 30 min post-exposure to 0.12 ng/ml at 12 h. A dose response mice exposure revealed a linear dose-dependent increase of ciguatoxin activity in mice blood, with more polar ciguatoxin congeners contributing to 89% of the total toxicity. Finally, the toxin measurement in mice blood exposed to toxic extracts from the Indian Ocean or from the Pacific Ocean showed that the blood collection card method could be extended to each of the three known ciguatoxin families (C-CTX, I-CTX and P-CTX). The low matrix effect of extracted dried-blood samples (used at 1:10 or 1:20 dilution) and the high sensitivity of the neuroblastoma assay (limit of detection 0.006 ng/ml C-CTX-1), determined that the blood collection card method is suitable to monitor ciguatoxin at sublethal doses in mice and opens the potential to be a useful procedure for fish screening, environmental risk assessment or clinical diagnosis of ciguatera fish poisoning in humans or marine mammals. SN - 0041-0101 UR - https://www.unboundmedicine.com/medline/citation/15979117/Biomonitoring_of_ciguatoxin_exposure_in_mice_using_blood_collection_cards_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-0101(05)00115-7 DB - PRIME DP - Unbound Medicine ER -