Tags

Type your tag names separated by a space and hit enter

Control of Alicyclobacillus acidoterrestris in fruit juices by enterocin AS-48.
Int J Food Microbiol. 2005 Oct 25; 104(3):289-97.IJ

Abstract

Alicyclobacillus acidoterrestris is a spoilage-causing bacterium in fruit juices. Control of this bacterium by enterocin AS-48 from Enterococcus faecalis A-48-32 is described. Enterocin AS-48 was active against one A. acidocaldarius and three strains of A. acidoterrestris tested. In natural orange and apple juices incubated at 37 degrees C, vegetative cells of A. acidoterrestris DSMZ 2,498 were inactivated by enterocin AS-48 (2.5 microg/ml) and no growth was observed in 14 days. In commercial fruit juices added of AS-48 (2.5 microg/ml) and inoculated with vegetative cells or with endospores of strain DSMZ 2,498, no viable cells were detected during 90 days of incubation at temperatures of 37 degrees C, 15 degrees C or 4 degrees C, except for apple, peach and grapefruit juices inoculated with vegetative cells and incubated at 37 degrees C which were protected efficiently for up to 60 days. Remarkably, in all commercial fruit juices tested, no viable cells were detected as early as 15 min after incubation with the bacteriocin. Endospores incubated for a very short time (1 min) with increasing bacteriocin concentrations were inactivated by 2.5 microg/ml AS-48. Electron microscopy examination of vegetative cells and endospores treated with enterocin AS-48 revealed substantial cell damage and bacterial lysis as well as disorganization of endospore structure.

Authors+Show Affiliations

Area de Microbiología, Departamento de Ciencias de la Salud, Facultad de Ciencias Experimentales, Universidad de Jaén, Campus Las Lagunillas s/n. 23071-Jaén, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15979752

Citation

Grande, Ma J., et al. "Control of Alicyclobacillus Acidoterrestris in Fruit Juices By Enterocin AS-48." International Journal of Food Microbiology, vol. 104, no. 3, 2005, pp. 289-97.
Grande MJ, Lucas R, Abriouel H, et al. Control of Alicyclobacillus acidoterrestris in fruit juices by enterocin AS-48. Int J Food Microbiol. 2005;104(3):289-97.
Grande, M. J., Lucas, R., Abriouel, H., Omar, N. B., Maqueda, M., Martínez-Bueno, M., Martínez-Cañamero, M., Valdivia, E., & Gálvez, A. (2005). Control of Alicyclobacillus acidoterrestris in fruit juices by enterocin AS-48. International Journal of Food Microbiology, 104(3), 289-97.
Grande MJ, et al. Control of Alicyclobacillus Acidoterrestris in Fruit Juices By Enterocin AS-48. Int J Food Microbiol. 2005 Oct 25;104(3):289-97. PubMed PMID: 15979752.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Control of Alicyclobacillus acidoterrestris in fruit juices by enterocin AS-48. AU - Grande,Ma J, AU - Lucas,R, AU - Abriouel,H, AU - Omar,N Ben, AU - Maqueda,M, AU - Martínez-Bueno,M, AU - Martínez-Cañamero,M, AU - Valdivia,E, AU - Gálvez,A, Y1 - 2005/06/24/ PY - 2004/04/30/received PY - 2005/01/21/revised PY - 2005/03/12/accepted PY - 2005/6/28/pubmed PY - 2005/12/16/medline PY - 2005/6/28/entrez SP - 289 EP - 97 JF - International journal of food microbiology JO - Int J Food Microbiol VL - 104 IS - 3 N2 - Alicyclobacillus acidoterrestris is a spoilage-causing bacterium in fruit juices. Control of this bacterium by enterocin AS-48 from Enterococcus faecalis A-48-32 is described. Enterocin AS-48 was active against one A. acidocaldarius and three strains of A. acidoterrestris tested. In natural orange and apple juices incubated at 37 degrees C, vegetative cells of A. acidoterrestris DSMZ 2,498 were inactivated by enterocin AS-48 (2.5 microg/ml) and no growth was observed in 14 days. In commercial fruit juices added of AS-48 (2.5 microg/ml) and inoculated with vegetative cells or with endospores of strain DSMZ 2,498, no viable cells were detected during 90 days of incubation at temperatures of 37 degrees C, 15 degrees C or 4 degrees C, except for apple, peach and grapefruit juices inoculated with vegetative cells and incubated at 37 degrees C which were protected efficiently for up to 60 days. Remarkably, in all commercial fruit juices tested, no viable cells were detected as early as 15 min after incubation with the bacteriocin. Endospores incubated for a very short time (1 min) with increasing bacteriocin concentrations were inactivated by 2.5 microg/ml AS-48. Electron microscopy examination of vegetative cells and endospores treated with enterocin AS-48 revealed substantial cell damage and bacterial lysis as well as disorganization of endospore structure. SN - 0168-1605 UR - https://www.unboundmedicine.com/medline/citation/15979752/Control_of_Alicyclobacillus_acidoterrestris_in_fruit_juices_by_enterocin_AS_48_ DB - PRIME DP - Unbound Medicine ER -