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Neuronal gap junctions in the mouse main olfactory bulb: morphological analyses on transgenic mice.
Neuroscience 2005; 134(3):757-69N

Abstract

In the present study we analyzed the structural features of extraglomerular gap junction-forming processes in mouse olfactory bulb electron microscopically. This work complements a previous study in which we analyzed the structural features of neuronal gap junction-forming processes within the glomerulus itself. Furthermore we examined connexin 36 expressing cells in the mouse olfactory bulb by analyzing transgenic mice in which the connexin 36 coding sequence was replaced with histological reporters. In extraglomerular regions, the mitral/tufted cell somata, dendrites and axon hillocks made gap junctions and mixed synapses with interneuronal processes. These gap junctions and synapses were associated with various types of interneuronal processes, including a particular type of sheet-like or calyx-like process contacting the somata or large dendrites of mitral/tufted cells. In the olfactory bulbs of the transgenic mice, connexin 36 was expressed in mitral cells, tufted cells, presumed granule cells and periglomerular cells. Multiple immunofluorescent labelings further revealed that presumed interneurons expressing connexin 36 in the periglomerular region rarely expressed calbindin, calretinin or tyrosine hydroxylase and are likely to comprise a chemically uncharacterized class of neurons. Similarly, interneurons expressing connexin 36 in the granule cell layer were rarely positive for calretinin, which was expressed in numerous presumed granule cells in the mouse main olfactory bulb. In summary, these findings revealed that mitral/tufted cells make gap junctions with diverse types of neurons; in the glomeruli gap junction-forming interneuronal processes originated from some types of periglomerular cells but others from a hitherto uncharacterized neuron type(s), and in the extraglomerular region gap-junction forming processes originate mainly from a subset of cells within the granule cell layer.

Authors+Show Affiliations

Department of Anatomy and Neurobiology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan. kosaka@a3rd.med.kyushu-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15979807

Citation

Kosaka, T, et al. "Neuronal Gap Junctions in the Mouse Main Olfactory Bulb: Morphological Analyses On Transgenic Mice." Neuroscience, vol. 134, no. 3, 2005, pp. 757-69.
Kosaka T, Deans MR, Paul DL, et al. Neuronal gap junctions in the mouse main olfactory bulb: morphological analyses on transgenic mice. Neuroscience. 2005;134(3):757-69.
Kosaka, T., Deans, M. R., Paul, D. L., & Kosaka, K. (2005). Neuronal gap junctions in the mouse main olfactory bulb: morphological analyses on transgenic mice. Neuroscience, 134(3), pp. 757-69.
Kosaka T, et al. Neuronal Gap Junctions in the Mouse Main Olfactory Bulb: Morphological Analyses On Transgenic Mice. Neuroscience. 2005;134(3):757-69. PubMed PMID: 15979807.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuronal gap junctions in the mouse main olfactory bulb: morphological analyses on transgenic mice. AU - Kosaka,T, AU - Deans,M R, AU - Paul,D L, AU - Kosaka,K, PY - 2005/03/08/received PY - 2005/04/25/revised PY - 2005/04/27/accepted PY - 2005/6/28/pubmed PY - 2005/11/15/medline PY - 2005/6/28/entrez SP - 757 EP - 69 JF - Neuroscience JO - Neuroscience VL - 134 IS - 3 N2 - In the present study we analyzed the structural features of extraglomerular gap junction-forming processes in mouse olfactory bulb electron microscopically. This work complements a previous study in which we analyzed the structural features of neuronal gap junction-forming processes within the glomerulus itself. Furthermore we examined connexin 36 expressing cells in the mouse olfactory bulb by analyzing transgenic mice in which the connexin 36 coding sequence was replaced with histological reporters. In extraglomerular regions, the mitral/tufted cell somata, dendrites and axon hillocks made gap junctions and mixed synapses with interneuronal processes. These gap junctions and synapses were associated with various types of interneuronal processes, including a particular type of sheet-like or calyx-like process contacting the somata or large dendrites of mitral/tufted cells. In the olfactory bulbs of the transgenic mice, connexin 36 was expressed in mitral cells, tufted cells, presumed granule cells and periglomerular cells. Multiple immunofluorescent labelings further revealed that presumed interneurons expressing connexin 36 in the periglomerular region rarely expressed calbindin, calretinin or tyrosine hydroxylase and are likely to comprise a chemically uncharacterized class of neurons. Similarly, interneurons expressing connexin 36 in the granule cell layer were rarely positive for calretinin, which was expressed in numerous presumed granule cells in the mouse main olfactory bulb. In summary, these findings revealed that mitral/tufted cells make gap junctions with diverse types of neurons; in the glomeruli gap junction-forming interneuronal processes originated from some types of periglomerular cells but others from a hitherto uncharacterized neuron type(s), and in the extraglomerular region gap-junction forming processes originate mainly from a subset of cells within the granule cell layer. SN - 0306-4522 UR - https://www.unboundmedicine.com/medline/citation/15979807/Neuronal_gap_junctions_in_the_mouse_main_olfactory_bulb:_morphological_analyses_on_transgenic_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0306-4522(05)00468-9 DB - PRIME DP - Unbound Medicine ER -