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Cell-mediated immune responses to influenza vaccination in healthy volunteers and allogeneic stem cell transplant recipients.
Bone Marrow Transplant 2005; 36(5):411-5BM

Abstract

Influenza is one of the most common respiratory diseases in humans. The response to vaccination is frequently poor in immunosuppressed individuals. The aim of the present study was to develop an enzyme-linked immunospot (ELISPOT) assay for measuring of the specific T-cell response to influenza vaccination. In all, 18 healthy subjects and six stem cell transplantation (SCT) patients tested before and 4 weeks after influenza vaccination were included in the present study. Peripheral blood lymphocytes were stimulated with four influenza peptides; three based on sequences from the hemagglutinin and one from the M1 protein. The ELISPOT assay and the measurement of intracellular IFN-gamma production were used to determine the cell-mediated responses after stimulation with the peptides. Influenza vaccination elicited strong cell-mediated immune responses in the healthy controls to all four peptides with 3.2-6.9-fold increases in the number of IFN-gamma producing spots/10(6) cells. By intracellular staining, it was suggested that CD4+ cells mediated the responses to the hemagglutinin peptides. In contrast, there was no increase in the number of IFN-gamma producing cells response after vaccination in the six SCT patients. In conclusion, our results suggest that the ELISPOT assay might be used as a complement to serology for monitoring of future influenza vaccine studies in SCT patients.

Authors+Show Affiliations

Department of Medicine, Division of Hematology, Karolinska Institutet, Stockholm, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15980884

Citation

Avetisyan, G, et al. "Cell-mediated Immune Responses to Influenza Vaccination in Healthy Volunteers and Allogeneic Stem Cell Transplant Recipients." Bone Marrow Transplantation, vol. 36, no. 5, 2005, pp. 411-5.
Avetisyan G, Ragnavölgyi E, Toth GT, et al. Cell-mediated immune responses to influenza vaccination in healthy volunteers and allogeneic stem cell transplant recipients. Bone Marrow Transplant. 2005;36(5):411-5.
Avetisyan, G., Ragnavölgyi, E., Toth, G. T., Hassan, M., & Ljungman, P. (2005). Cell-mediated immune responses to influenza vaccination in healthy volunteers and allogeneic stem cell transplant recipients. Bone Marrow Transplantation, 36(5), pp. 411-5.
Avetisyan G, et al. Cell-mediated Immune Responses to Influenza Vaccination in Healthy Volunteers and Allogeneic Stem Cell Transplant Recipients. Bone Marrow Transplant. 2005;36(5):411-5. PubMed PMID: 15980884.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cell-mediated immune responses to influenza vaccination in healthy volunteers and allogeneic stem cell transplant recipients. AU - Avetisyan,G, AU - Ragnavölgyi,E, AU - Toth,G T, AU - Hassan,M, AU - Ljungman,P, PY - 2005/6/28/pubmed PY - 2005/12/13/medline PY - 2005/6/28/entrez SP - 411 EP - 5 JF - Bone marrow transplantation JO - Bone Marrow Transplant. VL - 36 IS - 5 N2 - Influenza is one of the most common respiratory diseases in humans. The response to vaccination is frequently poor in immunosuppressed individuals. The aim of the present study was to develop an enzyme-linked immunospot (ELISPOT) assay for measuring of the specific T-cell response to influenza vaccination. In all, 18 healthy subjects and six stem cell transplantation (SCT) patients tested before and 4 weeks after influenza vaccination were included in the present study. Peripheral blood lymphocytes were stimulated with four influenza peptides; three based on sequences from the hemagglutinin and one from the M1 protein. The ELISPOT assay and the measurement of intracellular IFN-gamma production were used to determine the cell-mediated responses after stimulation with the peptides. Influenza vaccination elicited strong cell-mediated immune responses in the healthy controls to all four peptides with 3.2-6.9-fold increases in the number of IFN-gamma producing spots/10(6) cells. By intracellular staining, it was suggested that CD4+ cells mediated the responses to the hemagglutinin peptides. In contrast, there was no increase in the number of IFN-gamma producing cells response after vaccination in the six SCT patients. In conclusion, our results suggest that the ELISPOT assay might be used as a complement to serology for monitoring of future influenza vaccine studies in SCT patients. SN - 0268-3369 UR - https://www.unboundmedicine.com/medline/citation/15980884/Cell_mediated_immune_responses_to_influenza_vaccination_in_healthy_volunteers_and_allogeneic_stem_cell_transplant_recipients_ L2 - http://dx.doi.org/10.1038/sj.bmt.1705064 DB - PRIME DP - Unbound Medicine ER -