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Mechanisms of omega-3 fatty acid-induced growth inhibition in MDA-MB-231 human breast cancer cells.
Breast Cancer Res Treat 2005; 92(2):187-95BC

Abstract

The omega-3 fatty acids, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), inhibit the growth of human breast cancer cells in animal models and cell lines, but the mechanism by which this occurs is not well understood. In order to explore possible mechanisms for the modulation of breast cancer cell growth by omega-3 fatty acids, we examined the effects of EPA and DHA on the human breast cancer cell line MDA-MB-231. Omega-3 fatty acids (a combination of EPA and DHA) inhibited the growth of MDA-MB-231 cells by 30-40% (p<0.05) in both the presence and absence of linoleic acid, an essential omega-6 fatty acid. When provided individually, DHA was more potent than EPA in inhibiting the growth of MDA-MB-231 cells (p<0.05). EPA and DHA treatment decreased tumor cell proliferation (p<0.05), as estimated by decreased [methyl-(3)H]-thymidine uptake and expression of proliferation-associated proteins (proliferating cell nuclear antigen, PCNA, and proliferation-related kinase, PRK). In addition, EPA and DHA induced apoptosis, as indicated by a loss of mitochondrial membrane potential, increased caspase activity and increased DNA fragmentation (p<0.05). Cells incubated with omega-3 fatty acids demonstrated decreased Akt phosphorylation, as well as NFkappaB DNA binding activity (p<0.05). The results of this study indicate that omega-3 fatty acids decrease cell proliferation and induce apoptotic cell death in human breast cancer cells, possibly by decreasing signal transduction through the Akt/NFkappaB cell survival pathway.

Authors+Show Affiliations

Department of Agricultural,Food and Nutritional Science, University of Alberta, 3-18e Agriculture/Forestry Centre, Alberta, Edmonton, Canada.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Studies
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15986129

Citation

Schley, Patricia D., et al. "Mechanisms of Omega-3 Fatty Acid-induced Growth Inhibition in MDA-MB-231 Human Breast Cancer Cells." Breast Cancer Research and Treatment, vol. 92, no. 2, 2005, pp. 187-95.
Schley PD, Jijon HB, Robinson LE, et al. Mechanisms of omega-3 fatty acid-induced growth inhibition in MDA-MB-231 human breast cancer cells. Breast Cancer Res Treat. 2005;92(2):187-95.
Schley, P. D., Jijon, H. B., Robinson, L. E., & Field, C. J. (2005). Mechanisms of omega-3 fatty acid-induced growth inhibition in MDA-MB-231 human breast cancer cells. Breast Cancer Research and Treatment, 92(2), pp. 187-95.
Schley PD, et al. Mechanisms of Omega-3 Fatty Acid-induced Growth Inhibition in MDA-MB-231 Human Breast Cancer Cells. Breast Cancer Res Treat. 2005;92(2):187-95. PubMed PMID: 15986129.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mechanisms of omega-3 fatty acid-induced growth inhibition in MDA-MB-231 human breast cancer cells. AU - Schley,Patricia D, AU - Jijon,Humberto B, AU - Robinson,Lindsay E, AU - Field,Catherine J, PY - 2005/6/30/pubmed PY - 2005/10/26/medline PY - 2005/6/30/entrez SP - 187 EP - 95 JF - Breast cancer research and treatment JO - Breast Cancer Res. Treat. VL - 92 IS - 2 N2 - The omega-3 fatty acids, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), inhibit the growth of human breast cancer cells in animal models and cell lines, but the mechanism by which this occurs is not well understood. In order to explore possible mechanisms for the modulation of breast cancer cell growth by omega-3 fatty acids, we examined the effects of EPA and DHA on the human breast cancer cell line MDA-MB-231. Omega-3 fatty acids (a combination of EPA and DHA) inhibited the growth of MDA-MB-231 cells by 30-40% (p<0.05) in both the presence and absence of linoleic acid, an essential omega-6 fatty acid. When provided individually, DHA was more potent than EPA in inhibiting the growth of MDA-MB-231 cells (p<0.05). EPA and DHA treatment decreased tumor cell proliferation (p<0.05), as estimated by decreased [methyl-(3)H]-thymidine uptake and expression of proliferation-associated proteins (proliferating cell nuclear antigen, PCNA, and proliferation-related kinase, PRK). In addition, EPA and DHA induced apoptosis, as indicated by a loss of mitochondrial membrane potential, increased caspase activity and increased DNA fragmentation (p<0.05). Cells incubated with omega-3 fatty acids demonstrated decreased Akt phosphorylation, as well as NFkappaB DNA binding activity (p<0.05). The results of this study indicate that omega-3 fatty acids decrease cell proliferation and induce apoptotic cell death in human breast cancer cells, possibly by decreasing signal transduction through the Akt/NFkappaB cell survival pathway. SN - 0167-6806 UR - https://www.unboundmedicine.com/medline/citation/15986129/Mechanisms_of_omega_3_fatty_acid_induced_growth_inhibition_in_MDA_MB_231_human_breast_cancer_cells_ L2 - https://doi.org/10.1007/s10549-005-2415-z DB - PRIME DP - Unbound Medicine ER -