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Rapid modulation of inhibitory synaptic currents in cerebellar Purkinje cells by BDNF.
Synapse 2005; 57(4):183-90S

Abstract

The present study examined the acute effects of exogenous BDNF on inhibitory synaptic currents in Purkinje cells in cerebellar cultures. Miniature inhibitory postsynaptic currents (mIPSCs) were recorded in cultures (20-30 days in vitro), using discontinuous single electrode voltage clamp (dSEVC) technique. The effects of BDNF were studied in untreated control cultures and in cultures in which the endogenous levels of BDNF were decreased by chronic block of neural activity with tetrodotoxin (TTX). Chronic activity deprivation did not alter the amplitude of mIPSCs in Purkinje cells, and acute application of BDNF (50 ng/ml) to Purkinje cells in TTX-treated cultures significantly potentiated the amplitude and frequency of mIPSCs. By contrast, acute application of BDNF (50 ng/ml) produced no significant changes on mIPSC activity in control neurons. At higher concentrations of BDNF (100 ng/ml), comparable effects on mIPSC activity were also observed in control neurons. Preincubation of cerebellar cultures with K252a, an inhibitor of tyrosine kinases, effectively blocked the effects of BDNF on mIPSCs. These results indicate that functional inhibitory synapses develop in the absence of neural activity, and that activation of TrkB receptors by BDNF modulates inhibitory neurotransmission in Purkinje cells at both pre- and postsynaptic sites.

Authors+Show Affiliations

Neurology Research, Veterans Affairs Medical Center, Portland, Oregon 97239, USA. drakebar@ohsu.edu

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

15986361

Citation

Drake-Baumann, Rosemarie. "Rapid Modulation of Inhibitory Synaptic Currents in Cerebellar Purkinje Cells By BDNF." Synapse (New York, N.Y.), vol. 57, no. 4, 2005, pp. 183-90.
Drake-Baumann R. Rapid modulation of inhibitory synaptic currents in cerebellar Purkinje cells by BDNF. Synapse. 2005;57(4):183-90.
Drake-Baumann, R. (2005). Rapid modulation of inhibitory synaptic currents in cerebellar Purkinje cells by BDNF. Synapse (New York, N.Y.), 57(4), pp. 183-90.
Drake-Baumann R. Rapid Modulation of Inhibitory Synaptic Currents in Cerebellar Purkinje Cells By BDNF. Synapse. 2005 Sep 15;57(4):183-90. PubMed PMID: 15986361.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid modulation of inhibitory synaptic currents in cerebellar Purkinje cells by BDNF. A1 - Drake-Baumann,Rosemarie, PY - 2005/6/30/pubmed PY - 2005/10/28/medline PY - 2005/6/30/entrez SP - 183 EP - 90 JF - Synapse (New York, N.Y.) JO - Synapse VL - 57 IS - 4 N2 - The present study examined the acute effects of exogenous BDNF on inhibitory synaptic currents in Purkinje cells in cerebellar cultures. Miniature inhibitory postsynaptic currents (mIPSCs) were recorded in cultures (20-30 days in vitro), using discontinuous single electrode voltage clamp (dSEVC) technique. The effects of BDNF were studied in untreated control cultures and in cultures in which the endogenous levels of BDNF were decreased by chronic block of neural activity with tetrodotoxin (TTX). Chronic activity deprivation did not alter the amplitude of mIPSCs in Purkinje cells, and acute application of BDNF (50 ng/ml) to Purkinje cells in TTX-treated cultures significantly potentiated the amplitude and frequency of mIPSCs. By contrast, acute application of BDNF (50 ng/ml) produced no significant changes on mIPSC activity in control neurons. At higher concentrations of BDNF (100 ng/ml), comparable effects on mIPSC activity were also observed in control neurons. Preincubation of cerebellar cultures with K252a, an inhibitor of tyrosine kinases, effectively blocked the effects of BDNF on mIPSCs. These results indicate that functional inhibitory synapses develop in the absence of neural activity, and that activation of TrkB receptors by BDNF modulates inhibitory neurotransmission in Purkinje cells at both pre- and postsynaptic sites. SN - 0887-4476 UR - https://www.unboundmedicine.com/medline/citation/15986361/Rapid_modulation_of_inhibitory_synaptic_currents_in_cerebellar_Purkinje_cells_by_BDNF_ L2 - https://doi.org/10.1002/syn.20170 DB - PRIME DP - Unbound Medicine ER -