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Generation of a Bmp2 conditional null allele.
Genesis. 2005 Jul; 42(3):203-6.G

Abstract

Bone morphogenetic proteins (Bmp's) are known to play many important roles in embryogenesis. In addition, recent data from human genetic studies has revealed that Bmp's also have important functions in maintenance of the adult phenotype and aging. The original Bmp2 germline null allele resulted in lethality at embryonic day 7.0-10.5 due to malformation of the amnion/chorion and cardiac malformations. Because the early embryonic lethality of the Bmp2 germline null allele hinders further investigation into Bmp2 function at later stages, we generated a Bmp2 conditional null allele. Using gene targeting in mouse embryonic stem (ES) cells, we introduced LoxP sites upstream and downstream of Bmp2 exon 3 that encodes the mature peptide. Our results indicate that the Bmp2 conditional null allele is a true conditional null that encodes wildtype activity and reverts to a null allele after cre recombinase-induced recombination.

Authors+Show Affiliations

Alkek Institute of Biosciences and Technology, Texas A&M System Health Science Center, Houston, TX 77030, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15986484

Citation

Ma, Lijiang, and James F. Martin. "Generation of a Bmp2 Conditional Null Allele." Genesis (New York, N.Y. : 2000), vol. 42, no. 3, 2005, pp. 203-6.
Ma L, Martin JF. Generation of a Bmp2 conditional null allele. Genesis. 2005;42(3):203-6.
Ma, L., & Martin, J. F. (2005). Generation of a Bmp2 conditional null allele. Genesis (New York, N.Y. : 2000), 42(3), 203-6.
Ma L, Martin JF. Generation of a Bmp2 Conditional Null Allele. Genesis. 2005;42(3):203-6. PubMed PMID: 15986484.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Generation of a Bmp2 conditional null allele. AU - Ma,Lijiang, AU - Martin,James F, PY - 2005/6/30/pubmed PY - 2005/10/7/medline PY - 2005/6/30/entrez SP - 203 EP - 6 JF - Genesis (New York, N.Y. : 2000) JO - Genesis VL - 42 IS - 3 N2 - Bone morphogenetic proteins (Bmp's) are known to play many important roles in embryogenesis. In addition, recent data from human genetic studies has revealed that Bmp's also have important functions in maintenance of the adult phenotype and aging. The original Bmp2 germline null allele resulted in lethality at embryonic day 7.0-10.5 due to malformation of the amnion/chorion and cardiac malformations. Because the early embryonic lethality of the Bmp2 germline null allele hinders further investigation into Bmp2 function at later stages, we generated a Bmp2 conditional null allele. Using gene targeting in mouse embryonic stem (ES) cells, we introduced LoxP sites upstream and downstream of Bmp2 exon 3 that encodes the mature peptide. Our results indicate that the Bmp2 conditional null allele is a true conditional null that encodes wildtype activity and reverts to a null allele after cre recombinase-induced recombination. SN - 1526-954X UR - https://www.unboundmedicine.com/medline/citation/15986484/Generation_of_a_Bmp2_conditional_null_allele_ L2 - https://doi.org/10.1002/gene.20132 DB - PRIME DP - Unbound Medicine ER -