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On-line liquid chromatography electrospray ionization mass spectrometry for the characterization of kappa- and iota-carrageenans. Application to the hybrid iota-/nu-carrageenans.
Anal Chem. 2005 Jul 01; 77(13):4125-36.AC

Abstract

An on-line liquid chromatography electrospray ionization mass spectrometry (MS) method was developed for the characterization of polymers of kappa- (extracted from Kappaphycus alvarezii), iota-, and hybrid iota-/nu-carrageenans (both extracted from Eucheuma denticulatum) enzymatically digested with specific carrageenase enzymes. Applying either CID MS/MS or in-source fragmentation mechanisms, the results demonstrated that none of the polymers of kappa- or iota-carrageenans existed with their ideal repeating units. On the polymer of kappa-carrageenan, the nonideal structures identified consisted of iota-neocarrabiose sulfate units. On the polymer of iota-carrageenan, the nonideal structures identified consisted of the following: (i) kappa-neocarrabiose sulfate units, (ii) iota-neocarrabiose sulfate units with an additional sulfate group, and (iii) iota-neocarrabiose sulfate units with an additional sulfate and a pyruvate acetal group. For both kappa- and iota-carrageenans, the nonideal structures were randomly distributed on the polymers. The method was then applied for the characterization of a hybrid polymer of iota-/nu-carrageenans, enzymatically digested with iota-carrageenase. The results did not reveal an ideal oligosaccharide of nu-carrageenan, suggesting that the iota-carrageenase enzyme could cleave only reduced "densities" of nu-carrageenan repeating units. In addition, information about the sequence of hybrid iota-/nu-carrageenans from E. denticulatum is deduced.

Authors+Show Affiliations

UMR CNRS 6005, Institut de Chimie Organique et Analytique (ICOA), Université d'Orléans, BP 6759, 45067 Orléans Cedex 2, France.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15987118

Citation

Antonopoulos, Aristotelis, et al. "On-line Liquid Chromatography Electrospray Ionization Mass Spectrometry for the Characterization of Kappa- and Iota-carrageenans. Application to the Hybrid Iota-/nu-carrageenans." Analytical Chemistry, vol. 77, no. 13, 2005, pp. 4125-36.
Antonopoulos A, Favetta P, Helbert W, et al. On-line liquid chromatography electrospray ionization mass spectrometry for the characterization of kappa- and iota-carrageenans. Application to the hybrid iota-/nu-carrageenans. Anal Chem. 2005;77(13):4125-36.
Antonopoulos, A., Favetta, P., Helbert, W., & Lafosse, M. (2005). On-line liquid chromatography electrospray ionization mass spectrometry for the characterization of kappa- and iota-carrageenans. Application to the hybrid iota-/nu-carrageenans. Analytical Chemistry, 77(13), 4125-36.
Antonopoulos A, et al. On-line Liquid Chromatography Electrospray Ionization Mass Spectrometry for the Characterization of Kappa- and Iota-carrageenans. Application to the Hybrid Iota-/nu-carrageenans. Anal Chem. 2005 Jul 1;77(13):4125-36. PubMed PMID: 15987118.
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TY - JOUR T1 - On-line liquid chromatography electrospray ionization mass spectrometry for the characterization of kappa- and iota-carrageenans. Application to the hybrid iota-/nu-carrageenans. AU - Antonopoulos,Aristotelis, AU - Favetta,Patrick, AU - Helbert,William, AU - Lafosse,Michel, PY - 2005/7/1/pubmed PY - 2007/3/17/medline PY - 2005/7/1/entrez SP - 4125 EP - 36 JF - Analytical chemistry JO - Anal Chem VL - 77 IS - 13 N2 - An on-line liquid chromatography electrospray ionization mass spectrometry (MS) method was developed for the characterization of polymers of kappa- (extracted from Kappaphycus alvarezii), iota-, and hybrid iota-/nu-carrageenans (both extracted from Eucheuma denticulatum) enzymatically digested with specific carrageenase enzymes. Applying either CID MS/MS or in-source fragmentation mechanisms, the results demonstrated that none of the polymers of kappa- or iota-carrageenans existed with their ideal repeating units. On the polymer of kappa-carrageenan, the nonideal structures identified consisted of iota-neocarrabiose sulfate units. On the polymer of iota-carrageenan, the nonideal structures identified consisted of the following: (i) kappa-neocarrabiose sulfate units, (ii) iota-neocarrabiose sulfate units with an additional sulfate group, and (iii) iota-neocarrabiose sulfate units with an additional sulfate and a pyruvate acetal group. For both kappa- and iota-carrageenans, the nonideal structures were randomly distributed on the polymers. The method was then applied for the characterization of a hybrid polymer of iota-/nu-carrageenans, enzymatically digested with iota-carrageenase. The results did not reveal an ideal oligosaccharide of nu-carrageenan, suggesting that the iota-carrageenase enzyme could cleave only reduced "densities" of nu-carrageenan repeating units. In addition, information about the sequence of hybrid iota-/nu-carrageenans from E. denticulatum is deduced. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/15987118/On_line_liquid_chromatography_electrospray_ionization_mass_spectrometry_for_the_characterization_of_kappa__and_iota_carrageenans__Application_to_the_hybrid_iota_/nu_carrageenans_ L2 - https://doi.org/10.1021/ac050091o DB - PRIME DP - Unbound Medicine ER -