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Structural characterization of 2-aminobenzamide-derivatized oligosaccharides using a matrix-assisted laser desorption/ionization two-stage time-of-flight tandem mass spectrometer.
Rapid Commun Mass Spectrom. 2005; 19(14):2075-84.RC

Abstract

Oligosaccharides were derivatized by reductive amination using 2-aminobenzamide (2-AB) and analyzed by matrix-assisted laser desorption/ionization two-stage time-of-flight (MALDI-TOF/TOF) tandem mass spectrometry (MS/MS) in the positive ion mode. The major signals were obtained under these conditions from the [M+Na]+ ions for all 2-AB-derivatized oligosaccharides. A systematic study was conducted on a series of 2-AB-derivatized oligosaccharides to allow rationalization of the fragmentation processes. The MALDI-TOF/TOF-MS/MS spectra of the [M+Na]+ ions of 2-AB-derivatized oligosaccharides were dominated by glycosidic cleavages. These fragments originating both from the reducing and the non-reducing ends of the oligosaccharide yield information on sequence and branching. Moreover, the MALDI-TOF/TOF-MS/MS spectra were also characterized by abundant cross-ring fragments which are very informative on the linkages of the monosaccharide residues constituting these oligosaccharides. MALDI-TOF/TOF-MS/MS analysis of 2-AB-derivatized oligosaccharides, by providing structural information at the low-picomole level, appears to be a powerful tool for carbohydrate structural analysis.

Authors+Show Affiliations

Unité Mixte de Recherche CNRS/USTL 8576, "Glycobiologie Structurale et Fonctionnelle", IFR 118, Université des Sciences et Technologies de Lille 1, 59655 Villeneuve d'Ascq Cedex, France. willy.morelle@univ-lille1.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15988715

Citation

Morelle, Willy, et al. "Structural Characterization of 2-aminobenzamide-derivatized Oligosaccharides Using a Matrix-assisted Laser Desorption/ionization Two-stage Time-of-flight Tandem Mass Spectrometer." Rapid Communications in Mass Spectrometry : RCM, vol. 19, no. 14, 2005, pp. 2075-84.
Morelle W, Slomianny MC, Diemer H, et al. Structural characterization of 2-aminobenzamide-derivatized oligosaccharides using a matrix-assisted laser desorption/ionization two-stage time-of-flight tandem mass spectrometer. Rapid Commun Mass Spectrom. 2005;19(14):2075-84.
Morelle, W., Slomianny, M. C., Diemer, H., Schaeffer, C., van Dorsselaer, A., & Michalski, J. C. (2005). Structural characterization of 2-aminobenzamide-derivatized oligosaccharides using a matrix-assisted laser desorption/ionization two-stage time-of-flight tandem mass spectrometer. Rapid Communications in Mass Spectrometry : RCM, 19(14), 2075-84.
Morelle W, et al. Structural Characterization of 2-aminobenzamide-derivatized Oligosaccharides Using a Matrix-assisted Laser Desorption/ionization Two-stage Time-of-flight Tandem Mass Spectrometer. Rapid Commun Mass Spectrom. 2005;19(14):2075-84. PubMed PMID: 15988715.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Structural characterization of 2-aminobenzamide-derivatized oligosaccharides using a matrix-assisted laser desorption/ionization two-stage time-of-flight tandem mass spectrometer. AU - Morelle,Willy, AU - Slomianny,Marie-Christine, AU - Diemer,Hélène, AU - Schaeffer,Christine, AU - van Dorsselaer,Alain, AU - Michalski,Jean-Claude, PY - 2005/7/1/pubmed PY - 2005/9/1/medline PY - 2005/7/1/entrez SP - 2075 EP - 84 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 19 IS - 14 N2 - Oligosaccharides were derivatized by reductive amination using 2-aminobenzamide (2-AB) and analyzed by matrix-assisted laser desorption/ionization two-stage time-of-flight (MALDI-TOF/TOF) tandem mass spectrometry (MS/MS) in the positive ion mode. The major signals were obtained under these conditions from the [M+Na]+ ions for all 2-AB-derivatized oligosaccharides. A systematic study was conducted on a series of 2-AB-derivatized oligosaccharides to allow rationalization of the fragmentation processes. The MALDI-TOF/TOF-MS/MS spectra of the [M+Na]+ ions of 2-AB-derivatized oligosaccharides were dominated by glycosidic cleavages. These fragments originating both from the reducing and the non-reducing ends of the oligosaccharide yield information on sequence and branching. Moreover, the MALDI-TOF/TOF-MS/MS spectra were also characterized by abundant cross-ring fragments which are very informative on the linkages of the monosaccharide residues constituting these oligosaccharides. MALDI-TOF/TOF-MS/MS analysis of 2-AB-derivatized oligosaccharides, by providing structural information at the low-picomole level, appears to be a powerful tool for carbohydrate structural analysis. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/15988715/Structural_characterization_of_2_aminobenzamide_derivatized_oligosaccharides_using_a_matrix_assisted_laser_desorption/ionization_two_stage_time_of_flight_tandem_mass_spectrometer_ L2 - https://doi.org/10.1002/rcm.2033 DB - PRIME DP - Unbound Medicine ER -