Tags

Type your tag names separated by a space and hit enter

[Construction of a large human scFv library against SARS virus].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2005 Jul; 21(4):449-51, 455.XB

Abstract

AIM

To construct a large human scFv library against SARS virus by using in vivo recombination.

METHODS

Total RNA was isolated from the lymphocytes of 6 patients recovered from SARS. mRNA was isolated and reverse transcribed into cDNA. The V(H) and V(L) fragments were amplified from the cDNA and then assembled into scFv genes. The scFv genes were amplified and ligated into phagemid pDAN5. The primary library was constructed by transforming the recombinant phagemid into E.coli TG1. The secondary library was generated by in vivo recombination in E.coli BS1365 following the infection of BS1365 by primary library phages.

RESULTS

A primary library of 3x10(9) and a second library of 3x10(11) were constructed.

CONCLUSION

A large human scFv library against SARS virus with good diversity was constructed, which may be used for screening antibodies to SARS virus antigens.

Authors+Show Affiliations

Department of Immunology, Peking Union Medical University, Beijing 100005, China. zhaoaizhi@163.ccomNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

chi

PubMed ID

15989788

Citation

Zhao, Ai-zhi, et al. "[Construction of a Large Human scFv Library Against SARS Virus]." Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology, vol. 21, no. 4, 2005, pp. 449-51, 455.
Zhao AZ, Peng JQ, Cao H, et al. [Construction of a large human scFv library against SARS virus]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2005;21(4):449-51, 455.
Zhao, A. Z., Peng, J. Q., Cao, H., Kang, Y., Cui, L. X., Wu, X. B., & He, W. (2005). [Construction of a large human scFv library against SARS virus]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology, 21(4), 449-51, 455.
Zhao AZ, et al. [Construction of a Large Human scFv Library Against SARS Virus]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2005;21(4):449-51, 455. PubMed PMID: 15989788.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Construction of a large human scFv library against SARS virus]. AU - Zhao,Ai-zhi, AU - Peng,Jian-qiang, AU - Cao,Hui, AU - Kang,Yu, AU - Cui,Lian-xian, AU - Wu,Xiao-bing, AU - He,Wei, PY - 2005/7/2/pubmed PY - 2009/5/1/medline PY - 2005/7/2/entrez SP - 449-51, 455 JF - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology JO - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi VL - 21 IS - 4 N2 - AIM: To construct a large human scFv library against SARS virus by using in vivo recombination. METHODS: Total RNA was isolated from the lymphocytes of 6 patients recovered from SARS. mRNA was isolated and reverse transcribed into cDNA. The V(H) and V(L) fragments were amplified from the cDNA and then assembled into scFv genes. The scFv genes were amplified and ligated into phagemid pDAN5. The primary library was constructed by transforming the recombinant phagemid into E.coli TG1. The secondary library was generated by in vivo recombination in E.coli BS1365 following the infection of BS1365 by primary library phages. RESULTS: A primary library of 3x10(9) and a second library of 3x10(11) were constructed. CONCLUSION: A large human scFv library against SARS virus with good diversity was constructed, which may be used for screening antibodies to SARS virus antigens. SN - 1007-8738 UR - https://www.unboundmedicine.com/medline/citation/15989788/[Construction_of_a_large_human_scFv_library_against_SARS_virus]_ L2 - http://www.diseaseinfosearch.org/result/6415 DB - PRIME DP - Unbound Medicine ER -