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Re-evaluation of patients involved in a trichinellosis outbreak caused by Trichinella britovi 15 years after infection.
Vet Parasitol. 2005 Sep 05; 132(1-2):119-23.VP

Abstract

This study re-evaluates 13 out of 48 subjects involved in a trichinellosis outbreak that occurred in Central Italy (Umbria Region) in 1988 resulting from the consumption of raw boar meat harboring Trichinella britovi. During the outbreak, 28 of 48 serologically positive subjects were asymptomatic, whereas 20 subjects presented one or more clinical signs including but not limited to fever, myalgia, periorbital oedema and conjunctivitis. Several patients were hospitalized with severe clinical signs requiring treatment with mebendazole and corticosteroids. Upon re-evaluation of 13 patients, none presented clinical signs; however, three still had increased CPK or LDH serum levels with some signs of electromyographic changes. In this study, enzyme immunoassays (EIA) were used to test the 13 positive sera for reactivity with T. britovi antigens using both excretory/secretory (E/S) antigens and a synthetic antigen composed of beta-tyvelose conjugated to bovine serum albumin. Western blots (WB) were also carried out using a commercial kit. Studies using EIA with E/S antigen identified five positive sera; however, using beta-tyvelose as antigen, only one positive sample was identified. Nearly all sera reacted positively with one or more Trichinella antigens when analyzed by WB, in particular to the 45 k Da beta-tyvelose containing glycoprotein. Results indicate that T. britovi, though less pathogenic than other Trichinella species, is clearly capable of inducing sustainable sequelae.

Authors+Show Affiliations

Department of Veterinary Biopathological Sciences, Università di Perugia Via San Costanzo 4, 06100 Perugia, Italy.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15990231

Citation

Piergili-Fioretti, D, et al. "Re-evaluation of Patients Involved in a Trichinellosis Outbreak Caused By Trichinella Britovi 15 Years After Infection." Veterinary Parasitology, vol. 132, no. 1-2, 2005, pp. 119-23.
Piergili-Fioretti D, Castagna B, Frongillo RF, et al. Re-evaluation of patients involved in a trichinellosis outbreak caused by Trichinella britovi 15 years after infection. Vet Parasitol. 2005;132(1-2):119-23.
Piergili-Fioretti, D., Castagna, B., Frongillo, R. F., & Bruschi, F. (2005). Re-evaluation of patients involved in a trichinellosis outbreak caused by Trichinella britovi 15 years after infection. Veterinary Parasitology, 132(1-2), 119-23.
Piergili-Fioretti D, et al. Re-evaluation of Patients Involved in a Trichinellosis Outbreak Caused By Trichinella Britovi 15 Years After Infection. Vet Parasitol. 2005 Sep 5;132(1-2):119-23. PubMed PMID: 15990231.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Re-evaluation of patients involved in a trichinellosis outbreak caused by Trichinella britovi 15 years after infection. AU - Piergili-Fioretti,D, AU - Castagna,B, AU - Frongillo,R F, AU - Bruschi,F, PY - 2005/7/2/pubmed PY - 2005/11/16/medline PY - 2005/7/2/entrez SP - 119 EP - 23 JF - Veterinary parasitology JO - Vet Parasitol VL - 132 IS - 1-2 N2 - This study re-evaluates 13 out of 48 subjects involved in a trichinellosis outbreak that occurred in Central Italy (Umbria Region) in 1988 resulting from the consumption of raw boar meat harboring Trichinella britovi. During the outbreak, 28 of 48 serologically positive subjects were asymptomatic, whereas 20 subjects presented one or more clinical signs including but not limited to fever, myalgia, periorbital oedema and conjunctivitis. Several patients were hospitalized with severe clinical signs requiring treatment with mebendazole and corticosteroids. Upon re-evaluation of 13 patients, none presented clinical signs; however, three still had increased CPK or LDH serum levels with some signs of electromyographic changes. In this study, enzyme immunoassays (EIA) were used to test the 13 positive sera for reactivity with T. britovi antigens using both excretory/secretory (E/S) antigens and a synthetic antigen composed of beta-tyvelose conjugated to bovine serum albumin. Western blots (WB) were also carried out using a commercial kit. Studies using EIA with E/S antigen identified five positive sera; however, using beta-tyvelose as antigen, only one positive sample was identified. Nearly all sera reacted positively with one or more Trichinella antigens when analyzed by WB, in particular to the 45 k Da beta-tyvelose containing glycoprotein. Results indicate that T. britovi, though less pathogenic than other Trichinella species, is clearly capable of inducing sustainable sequelae. SN - 0304-4017 UR - https://www.unboundmedicine.com/medline/citation/15990231/Re_evaluation_of_patients_involved_in_a_trichinellosis_outbreak_caused_by_Trichinella_britovi_15_years_after_infection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-4017(05)00270-0 DB - PRIME DP - Unbound Medicine ER -