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Bet v 1142-156 is the dominant T-cell epitope of the major birch pollen allergen and important for cross-reactivity with Bet v 1-related food allergens.
J Allergy Clin Immunol. 2005 Jul; 116(1):213-9.JA

Abstract

BACKGROUND

Individuals with birch pollen allergy frequently experience hypersensitivity reactions to certain foods, primarily because of IgE antibodies specific for the major birch pollen allergen Bet v 1 that cross-react with homologous food allergens.

OBJECTIVE

We sought to characterize the major T-cell epitopes of Bet v 1 and to investigate their involvement in the cellular cross-reactivity with homologous food allergens.

METHODS

T-cell epitope mapping of Bet v 1 was performed by testing Bet v 1-specific T-cell lines derived from 57 individuals with birch pollen allergy, with overlapping peptides representing the entire allergen. T-cell lines and T-cell clones were stimulated with Bet v 1-related major allergens from apple (Mal d 1), cherry (Pru av 1), hazelnut (Cor a 1), celery (Api g 1), carrot (Dau c 1), and soybean (Gly m 4) and with peptides deduced from the C-terminal amino acid sequences of these molecules. Results Bet v 1 142-156 , positioned in the highly conserved C-terminal region of Bet v 1, was identified as the major T-cell epitope recognized by 61% of individuals. Most T lymphocytes specific for Bet v 1 142-156 were activated by one or more homologous food proteins or the respective peptides, as indicated by proliferation and cytokine production.

CONCLUSION

The major T-cell epitope of Bet v 1, Bet v 1 142-156 , plays an important role in the cellular cross-reactivity between this respiratory allergen and related food allergens. Thus T lymphocytes specific for Bet v 1 142-156 might be activated by various Bet v 1-related food allergens in vivo, even out of the pollen season.

Authors+Show Affiliations

Department of Pathophysiology, Center of Physiology and Pathophysiology, Medical University of Vienna, Austria.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15990797

Citation

Jahn-Schmid, Beatrice, et al. "Bet V 1142-156 Is the Dominant T-cell Epitope of the Major Birch Pollen Allergen and Important for Cross-reactivity With Bet V 1-related Food Allergens." The Journal of Allergy and Clinical Immunology, vol. 116, no. 1, 2005, pp. 213-9.
Jahn-Schmid B, Radakovics A, Lüttkopf D, et al. Bet v 1142-156 is the dominant T-cell epitope of the major birch pollen allergen and important for cross-reactivity with Bet v 1-related food allergens. J Allergy Clin Immunol. 2005;116(1):213-9.
Jahn-Schmid, B., Radakovics, A., Lüttkopf, D., Scheurer, S., Vieths, S., Ebner, C., & Bohle, B. (2005). Bet v 1142-156 is the dominant T-cell epitope of the major birch pollen allergen and important for cross-reactivity with Bet v 1-related food allergens. The Journal of Allergy and Clinical Immunology, 116(1), 213-9.
Jahn-Schmid B, et al. Bet V 1142-156 Is the Dominant T-cell Epitope of the Major Birch Pollen Allergen and Important for Cross-reactivity With Bet V 1-related Food Allergens. J Allergy Clin Immunol. 2005;116(1):213-9. PubMed PMID: 15990797.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bet v 1142-156 is the dominant T-cell epitope of the major birch pollen allergen and important for cross-reactivity with Bet v 1-related food allergens. AU - Jahn-Schmid,Beatrice, AU - Radakovics,Astrid, AU - Lüttkopf,Dirk, AU - Scheurer,Stephan, AU - Vieths,Stefan, AU - Ebner,Christof, AU - Bohle,Barbara, PY - 2005/7/2/pubmed PY - 2005/8/10/medline PY - 2005/7/2/entrez SP - 213 EP - 9 JF - The Journal of allergy and clinical immunology JO - J Allergy Clin Immunol VL - 116 IS - 1 N2 - BACKGROUND: Individuals with birch pollen allergy frequently experience hypersensitivity reactions to certain foods, primarily because of IgE antibodies specific for the major birch pollen allergen Bet v 1 that cross-react with homologous food allergens. OBJECTIVE: We sought to characterize the major T-cell epitopes of Bet v 1 and to investigate their involvement in the cellular cross-reactivity with homologous food allergens. METHODS: T-cell epitope mapping of Bet v 1 was performed by testing Bet v 1-specific T-cell lines derived from 57 individuals with birch pollen allergy, with overlapping peptides representing the entire allergen. T-cell lines and T-cell clones were stimulated with Bet v 1-related major allergens from apple (Mal d 1), cherry (Pru av 1), hazelnut (Cor a 1), celery (Api g 1), carrot (Dau c 1), and soybean (Gly m 4) and with peptides deduced from the C-terminal amino acid sequences of these molecules. Results Bet v 1 142-156 , positioned in the highly conserved C-terminal region of Bet v 1, was identified as the major T-cell epitope recognized by 61% of individuals. Most T lymphocytes specific for Bet v 1 142-156 were activated by one or more homologous food proteins or the respective peptides, as indicated by proliferation and cytokine production. CONCLUSION: The major T-cell epitope of Bet v 1, Bet v 1 142-156 , plays an important role in the cellular cross-reactivity between this respiratory allergen and related food allergens. Thus T lymphocytes specific for Bet v 1 142-156 might be activated by various Bet v 1-related food allergens in vivo, even out of the pollen season. SN - 0091-6749 UR - https://www.unboundmedicine.com/medline/citation/15990797/Bet_v_1142_156_is_the_dominant_T_cell_epitope_of_the_major_birch_pollen_allergen_and_important_for_cross_reactivity_with_Bet_v_1_related_food_allergens_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0091674905007621 DB - PRIME DP - Unbound Medicine ER -