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Induction of CX3CL1 expression in astrocytes and CX3CR1 in microglia in the spinal cord of a rat model of neuropathic pain.
J Pain. 2005 Jul; 6(7):434-8.JP

Abstract

Previous studies have shown that chemokines might play a role in the pathology of chronic pain. The purpose of this study was to provide an immunohistochemical description of the distribution of CX3CL1 (fractalkine) and its receptor CX3CR1 in the rat spinal cord in a model of inflammatory pain induced by unilateral intraplantar complete Freund's adjuvant (CFA) and in a model of neuropathic pain induced by L5 spinal nerve ligation (modified Chung model or mSNL). In naïve rats, CX3CL1 is found in the cytoplasm of neurons as shown by colocalization of CX3XL1 and NeuN. Similar distribution of CX3CL1 was observed after CFA, whereas after mSNL, CX3CL1 was not only observed in neurons but also found in astrocytes, as shown by colocalization of CX3CL1 and GFAP. Weak immunoreactivity for the CX3CL1 receptor, CX3CR1, was found in microglia in the spinal cord of either naïve rats or rats with inflammation. However, after spinal nerve injury, CX3CR1-LI was upregulated in microglia throughout the dorsal horn.

PERSPECTIVE

This study shows that spinal nerve injury, but not peripheral inflammation, induces the expression of a chemokine, CX3CL1 (fractalkine), in astrocytes and upregulates CX3CR1 in microglia in the spinal cord. This selective regulation of CX3CL1 and its receptor, CX3CR1, suggests that these chemokines may represent new targets for the treatment of neuropathic pain.

Authors+Show Affiliations

Department of Pharmacology, Merck Research Laboratories, Rahway, New Jersey 07065, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15993821

Citation

Lindia, Jill A., et al. "Induction of CX3CL1 Expression in Astrocytes and CX3CR1 in Microglia in the Spinal Cord of a Rat Model of Neuropathic Pain." The Journal of Pain, vol. 6, no. 7, 2005, pp. 434-8.
Lindia JA, McGowan E, Jochnowitz N, et al. Induction of CX3CL1 expression in astrocytes and CX3CR1 in microglia in the spinal cord of a rat model of neuropathic pain. J Pain. 2005;6(7):434-8.
Lindia, J. A., McGowan, E., Jochnowitz, N., & Abbadie, C. (2005). Induction of CX3CL1 expression in astrocytes and CX3CR1 in microglia in the spinal cord of a rat model of neuropathic pain. The Journal of Pain, 6(7), 434-8.
Lindia JA, et al. Induction of CX3CL1 Expression in Astrocytes and CX3CR1 in Microglia in the Spinal Cord of a Rat Model of Neuropathic Pain. J Pain. 2005;6(7):434-8. PubMed PMID: 15993821.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Induction of CX3CL1 expression in astrocytes and CX3CR1 in microglia in the spinal cord of a rat model of neuropathic pain. AU - Lindia,Jill A, AU - McGowan,Erin, AU - Jochnowitz,Nina, AU - Abbadie,Catherine, PY - 2005/01/04/received PY - 2005/01/25/revised PY - 2005/02/03/accepted PY - 2005/7/5/pubmed PY - 2005/9/9/medline PY - 2005/7/5/entrez SP - 434 EP - 8 JF - The journal of pain JO - J Pain VL - 6 IS - 7 N2 - UNLABELLED: Previous studies have shown that chemokines might play a role in the pathology of chronic pain. The purpose of this study was to provide an immunohistochemical description of the distribution of CX3CL1 (fractalkine) and its receptor CX3CR1 in the rat spinal cord in a model of inflammatory pain induced by unilateral intraplantar complete Freund's adjuvant (CFA) and in a model of neuropathic pain induced by L5 spinal nerve ligation (modified Chung model or mSNL). In naïve rats, CX3CL1 is found in the cytoplasm of neurons as shown by colocalization of CX3XL1 and NeuN. Similar distribution of CX3CL1 was observed after CFA, whereas after mSNL, CX3CL1 was not only observed in neurons but also found in astrocytes, as shown by colocalization of CX3CL1 and GFAP. Weak immunoreactivity for the CX3CL1 receptor, CX3CR1, was found in microglia in the spinal cord of either naïve rats or rats with inflammation. However, after spinal nerve injury, CX3CR1-LI was upregulated in microglia throughout the dorsal horn. PERSPECTIVE: This study shows that spinal nerve injury, but not peripheral inflammation, induces the expression of a chemokine, CX3CL1 (fractalkine), in astrocytes and upregulates CX3CR1 in microglia in the spinal cord. This selective regulation of CX3CL1 and its receptor, CX3CR1, suggests that these chemokines may represent new targets for the treatment of neuropathic pain. SN - 1526-5900 UR - https://www.unboundmedicine.com/medline/citation/15993821/Induction_of_CX3CL1_expression_in_astrocytes_and_CX3CR1_in_microglia_in_the_spinal_cord_of_a_rat_model_of_neuropathic_pain_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1526-5900(05)00418-9 DB - PRIME DP - Unbound Medicine ER -