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The effect of ethanol on the physical properties of neuronal membranes.
Mol Cells. 2005 Jun 30; 19(3):356-64.MC

Abstract

Intramolecular excimer formation of 1,3-di(1-pyrenyl) propane(Py-3-Py) and fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene (DPH) were used to evaluate the effect of ethanol on the rate and range of lateral and rotational mobilities of bulk bilayer structures of synaptosomal plasma membrane vesicles (SPMVs) from the bovine cerebral cortex. Ethanol increased the excimer to monomer fluorescence intensity ratio (I'/I) of Py-3-Py in the SPMVs. Selective quenching of both DPH and Py-3-Py by trinitrophenyl groups was used to examine the range of transbilayer asymmetric rotational mobility and the rate and range of transbilayer asymmetric lateral mobility of SPMVs. Ethanol increased the rotational and lateral mobility of the outer monolayer more than of the inner one. Thus ethanol has a selective fluidizing effect within the transbilayer domains of the SPMVs. Radiationless energy transfer from the tryptophans of membrane proteins to Py-3-Py was used to examine both the effect of ethanol on annular lipid fluidity and protein distribution in the SPMVs. Ethanol increased annular lipid fluidity and also caused membrane proteins to cluster. These effects on neuronal membranes may be responsible for some, though not all, of the general anesthetic actions of ethanol.

Authors+Show Affiliations

Department of Dental Pharmacology and Biophysics, College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University, Busan, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15995352

Citation

Bae, Moon-Kyoung, et al. "The Effect of Ethanol On the Physical Properties of Neuronal Membranes." Molecules and Cells, vol. 19, no. 3, 2005, pp. 356-64.
Bae MK, Jeong DK, Park NS, et al. The effect of ethanol on the physical properties of neuronal membranes. Mol Cells. 2005;19(3):356-64.
Bae, M. K., Jeong, D. K., Park, N. S., Lee, C. H., Cho, B. H., Jang, H. O., & Yun, I. (2005). The effect of ethanol on the physical properties of neuronal membranes. Molecules and Cells, 19(3), 356-64.
Bae MK, et al. The Effect of Ethanol On the Physical Properties of Neuronal Membranes. Mol Cells. 2005 Jun 30;19(3):356-64. PubMed PMID: 15995352.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of ethanol on the physical properties of neuronal membranes. AU - Bae,Moon-Kyoung, AU - Jeong,Dong-Keun, AU - Park,No-Soo, AU - Lee,Cheol-Ho, AU - Cho,Bong-Hye, AU - Jang,Hye-Ock, AU - Yun,Il, PY - 2005/7/5/pubmed PY - 2005/10/28/medline PY - 2005/7/5/entrez SP - 356 EP - 64 JF - Molecules and cells JO - Mol Cells VL - 19 IS - 3 N2 - Intramolecular excimer formation of 1,3-di(1-pyrenyl) propane(Py-3-Py) and fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene (DPH) were used to evaluate the effect of ethanol on the rate and range of lateral and rotational mobilities of bulk bilayer structures of synaptosomal plasma membrane vesicles (SPMVs) from the bovine cerebral cortex. Ethanol increased the excimer to monomer fluorescence intensity ratio (I'/I) of Py-3-Py in the SPMVs. Selective quenching of both DPH and Py-3-Py by trinitrophenyl groups was used to examine the range of transbilayer asymmetric rotational mobility and the rate and range of transbilayer asymmetric lateral mobility of SPMVs. Ethanol increased the rotational and lateral mobility of the outer monolayer more than of the inner one. Thus ethanol has a selective fluidizing effect within the transbilayer domains of the SPMVs. Radiationless energy transfer from the tryptophans of membrane proteins to Py-3-Py was used to examine both the effect of ethanol on annular lipid fluidity and protein distribution in the SPMVs. Ethanol increased annular lipid fluidity and also caused membrane proteins to cluster. These effects on neuronal membranes may be responsible for some, though not all, of the general anesthetic actions of ethanol. SN - 1016-8478 UR - https://www.unboundmedicine.com/medline/citation/15995352/The_effect_of_ethanol_on_the_physical_properties_of_neuronal_membranes_ L2 - http://www.molcells.org/journal/view.html?year=2005&volume=19&number=3&spage=356 DB - PRIME DP - Unbound Medicine ER -