Rapid determination of aniline metabolites of chlorpropham in potatoes by micellar electrokinetic chromatography using negative-charged mixed micelles and laser-induced fluorescence detection.
Electrophoresis. 2005 Aug; 26(15):2991-8.E

Abstract

A rapid, reliable method has been developed for the multi-residue analysis of aniline metabolites of chlorpropham in potato samples. The method involves the precolumn derivatization of aniline metabolites with 5-(4,6-dichloro-s-triazin-2-ylamino) fluorescein (DTAF) and their subsequent separation and determination by micellar electrokinetic capillary chromatography with laser-induced fluorescence detection (MEKC-LIF). The optimum procedure includes a derivatization step of the aniline metabolites (3-chloroaniline, 3-chloro-4-hydroxyaniline and 3-chloro-4-methoxyaniline) at 40 degrees C for 40 min and a 5-fold dilution prior to MEKC analysis, which is conducted within about 7 min using negative-charged mixed micelles (SDS/Triton X-100) in the running buffer. Under these conditions, the DTAF-anilines were readily detected at 0.3-3.1 microg/L level with a precision of 4.8-6.4%. These results indicate that negative-charged mixed surfactant MEKC-LIF is useful as a selective, rapid, and sensitive tool for the determination of these anilines and surpasses other electrophoretic alternatives based on the use of fluorescein-isothiocyanate (FITC) as label reagent. Finally, the potato matrix showed no significant effects on the derivatization and determination of these analytes, since the analytical figures of merit for the real samples were similar to those obtained in aqueous solutions, and the average recovery at fortification levels of 10-250 microg/kg was over 97%.

Authors+Show Affiliations

Orejuela E

Department of Analytical Chemistry, Marie-Curie Building (Annex), Rabanales Campus, University of Cordoba, Spain.

Silva M

No affiliation info available

MeSH

AminophenolsAniline CompoundsChlorprophamChromatography, Micellar Electrokinetic CapillaryFluoresceinsFluorescenceFluorescent DyesFood AnalysisLasersMicellesOctoxynolPesticidesSodium Dodecyl SulfateSolanum tuberosum

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15995980

Citation

Orejuela, Eva, and Manuel Silva. "Rapid Determination of Aniline Metabolites of Chlorpropham in Potatoes By Micellar Electrokinetic Chromatography Using Negative-charged Mixed Micelles and Laser-induced Fluorescence Detection." Electrophoresis, vol. 26, no. 15, 2005, pp. 2991-8.

Orejuela E, Silva M. Rapid determination of aniline metabolites of chlorpropham in potatoes by micellar electrokinetic chromatography using negative-charged mixed micelles and laser-induced fluorescence detection. Electrophoresis. 2005;26(15):2991-8.

Orejuela, E., & Silva, M. (2005). Rapid determination of aniline metabolites of chlorpropham in potatoes by micellar electrokinetic chromatography using negative-charged mixed micelles and laser-induced fluorescence detection. Electrophoresis, 26(15), 2991-8.

Orejuela E, Silva M. Rapid Determination of Aniline Metabolites of Chlorpropham in Potatoes By Micellar Electrokinetic Chromatography Using Negative-charged Mixed Micelles and Laser-induced Fluorescence Detection. Electrophoresis. 2005;26(15):2991-8. PubMed PMID: 15995980.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Rapid determination of aniline metabolites of chlorpropham in potatoes by micellar electrokinetic chromatography using negative-charged mixed micelles and laser-induced fluorescence detection. AU - Orejuela,Eva, AU - Silva,Manuel, PY - 2005/7/5/pubmed PY - 2006/3/3/medline PY - 2005/7/5/entrez SP - 2991 EP - 8 JF - Electrophoresis JO - Electrophoresis VL - 26 IS - 15 N2 - A rapid, reliable method has been developed for the multi-residue analysis of aniline metabolites of chlorpropham in potato samples. The method involves the precolumn derivatization of aniline metabolites with 5-(4,6-dichloro-s-triazin-2-ylamino) fluorescein (DTAF) and their subsequent separation and determination by micellar electrokinetic capillary chromatography with laser-induced fluorescence detection (MEKC-LIF). The optimum procedure includes a derivatization step of the aniline metabolites (3-chloroaniline, 3-chloro-4-hydroxyaniline and 3-chloro-4-methoxyaniline) at 40 degrees C for 40 min and a 5-fold dilution prior to MEKC analysis, which is conducted within about 7 min using negative-charged mixed micelles (SDS/Triton X-100) in the running buffer. Under these conditions, the DTAF-anilines were readily detected at 0.3-3.1 microg/L level with a precision of 4.8-6.4%. These results indicate that negative-charged mixed surfactant MEKC-LIF is useful as a selective, rapid, and sensitive tool for the determination of these anilines and surpasses other electrophoretic alternatives based on the use of fluorescein-isothiocyanate (FITC) as label reagent. Finally, the potato matrix showed no significant effects on the derivatization and determination of these analytes, since the analytical figures of merit for the real samples were similar to those obtained in aqueous solutions, and the average recovery at fortification levels of 10-250 microg/kg was over 97%. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/15995980/Rapid_determination_of_aniline_metabolites_of_chlorpropham_in_potatoes_by_micellar_electrokinetic_chromatography_using_negative_charged_mixed_micelles_and_laser_induced_fluorescence_detection_ DB - PRIME DP - Unbound Medicine ER -

Tags

Rapid determination of aniline metabolites of chlorpropham in potatoes by micellar electrokinetic chromatography using negative-charged mixed micelles and laser-induced fluorescence detection.Electrophoresis. 2005 Aug; 26(15):2991-8.E